EC Number   |
|---|
   3.5.1.52 | - |
| 3.5.1.52 | 14500fold |
| 3.5.1.52 | 460fold |
| 3.5.1.52 | glutathione-Sepharose column chromatography |
| 3.5.1.52 | native enzyme by Fe2+ affinity and phosphopeptide affinity chromatography to over 95% purity |
| 3.5.1.52 | Ni-nitrilotriacetate column chromatography |
| 3.5.1.52 | recombinant full-length enzyme, enzyme core domain, and enzyme XPCB domain from Escherichia coli strain BL21(DE3),the XPCB domain by chitin affinity chromatographyand gel filtration |
| 3.5.1.52 | recombinant GST-tagged proteasome components HR23B, S4, and AMFR from Escherichia coli by glutathione affinity batch method, recombinant His6-tagged wild-type enzyme and truncated variants from Escherichia coli by nickel affinity chromatography |
| 3.5.1.52 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinit chromatography |
| 3.5.1.52 | recombinant His-tagged enzyme PNGase Yl from Pichia pastoris strain GS115 by ultracentrifugatio, dialysis, and nickel affinity chromatography |
