EC Number   |
|---|
   3.4.24.27 | - |
| 3.4.24.27 | by affinity chromatography |
| 3.4.24.27 | by Gly-D-Phe affinity chromatography, coupling to the resin by epichlorohydrin, 1,4-butandiol diglycidyl ether, or 1,6-hexanediol diglycidyl ether, method optimization and evaluation, overview |
| 3.4.24.27 | commercial enzyme powder further purified to remove salts |
| 3.4.24.27 | commercial preparation, used without further purification |
| 3.4.24.27 | evaluation of Gly-D-Phe, Gly-L-Leu, and D-Phe as affinity ligands for thermolysin, each of the ligands is immobilized to a resin. The optimum pH for adsorption of thermolysin is pH 5.0 to pH 6.0 for each of the ligands, affinity chromatography method development, adsorption isotherms, overview |
| 3.4.24.27 | expression as single polypeptide pre-proenzyme in Escherichia coli, secretion into medium as mature enzyme |
| 3.4.24.27 | incubation of impure enzyme at 37°C causes proteolysis of impurities, whereas the protease remains active and uncleaved |
| 3.4.24.27 | mobile phase effects in the high-performance affinity purification |
| 3.4.24.27 | native enzyme to homogeneity from the culture supernatant by ammonium sulfate fractionation, anion exchange chromatography, gel filtration |
