EC Number   |
|---|
   3.4.21.68 | - |
| 3.4.21.68 | glycosylation variant of human tPA |
| 3.4.21.68 | mutant N117Q/N184Q |
| 3.4.21.68 | one-step purification using affinity chromatography with a special monoclonal antibody |
| 3.4.21.68 | recombinant active full-length and active truncated 650 bp enzymes from Nicotiana tabacum by lysine affinity chromatography and gel filtration |
| 3.4.21.68 | recombinant chimeric tissue plasminogen activator mutant from Nicotiana tabacum hairy roots by lysine affinity chromatography. In addition, specific aptamers are designed using SELEX method. Multi wall carbon nanotubes are functionalized with selected aptamers, Chi-tPA1-5, packed in a column, and used for purification. The selected aptamer Chi-tPA1, having KD values of 0.320 nM and IC50 of 28.9 nM, possesses good affinity to tPA, and the chimeric mutant tPA is properly purified by aptamer-chromatography. Development of aptamer-based affinity purification method, overview |
| 3.4.21.68 | recombinant enzyme |
| 3.4.21.68 | recombinant His-tagged kringle 2 plus serine protease domains, K2S, of human tissue plasminogen activator from Escherichia coli by nickel affinity chromatography after cleavage with factor Xa |
| 3.4.21.68 | recombinant His-tagged soluble full-length enzyme tPA from Escherichia coli by nickel affinity chromatography, the His-tag is cleaved off by PreScission Protease (PSP), leaving only two additional residues (Gly and Pro) at the N-terminus, followed by gel filtration |
| 3.4.21.68 | recombinant His-tagged truncated enzyme from Leishmania tarentolae by nickel affinity chromatography |
