EC Number |
---|
3.2.1.21 | - |
3.2.1.21 | 117fold to homogeneity from fresh leaves by chilled acetone and ammonium sulfate precipitation, ion exchange chromatography and gel filtration |
3.2.1.21 | 18fold by ammonium sulfate fractionation, ion exchange and adsorption chromatography, and gel filtration to homogeneity |
3.2.1.21 | a single step concanvalin A affinity chromatography for native PRGH1 improves the yield and reduced the purification time |
3.2.1.21 | acetone precipitation, DEAE-cellulose |
3.2.1.21 | affinity column chromatography |
3.2.1.21 | after growth on 2% microcrystalline cellulose |
3.2.1.21 | after growth on cellulose |
3.2.1.21 | ammonium sulfate precipitation and Sepharose-4B-L-tyrosine-1-naphthylamine column chromatography |
3.2.1.21 | ammonium sulfate precipitation, butyl Sepharose column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration |