EC Number   |
|---|
   3.1.3.56 | - |
| 3.1.3.56 | His-tag fusion protein expressed in Escherichia coli |
| 3.1.3.56 | membrane associated enzyme |
| 3.1.3.56 | partial purification of membrane bound and soluble enzyme |
| 3.1.3.56 | partial purification of membrane bound enzyme |
| 3.1.3.56 | partial purification of type I enzyme |
| 3.1.3.56 | purification by a dual tag strategy: cDNA encoding human Type I Ins(1,4,5)P3 5-phosphatase is subcloned into a modified pMAL expression vector. This plasmid produces a recombinant protein in fusion with affinity tags located at its N-terminus, consisting in a maltose binding protein (MPB) and an octa-histidine stretch. The construction is transformed into Escherichia coli BL21 (DE3) expression strain. Yield: 18 mg of pure and stable enzyme starting from a 2 L |
| 3.1.3.56 | recombinant enzyme using His-tag |
| 3.1.3.56 | recombinant enzymes from Escherichia coli using His-tag |
| 3.1.3.56 | recombinant His-tagged catalytic domain from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the tag is cleaved off |
