EC Number   |
|---|
   3.1.1.25 | - |
| 3.1.1.25 | ammonium sulfate precipitation, DEAE-Trisacryl M column chromatography, Sephacryl S300HR gel filtration, and Cibacron Blue 3GA gel filtration |
| 3.1.1.25 | anion-exchange chromatography and concanavalin-A chromatography |
| 3.1.1.25 | by different types of chromatographies |
| 3.1.1.25 | by pseudo-affinity chromatography and gel filtration |
| 3.1.1.25 | Ni-NTA column chromatography |
| 3.1.1.25 | process of ultrafiltration, acetone precipitation, anion-exchange, hydrophobic and hydroxyapatite chromatography |
| 3.1.1.25 | Q-Sepharose column chromatography |
| 3.1.1.25 | the presence of 2.5 mM Ca2+ and 0.1% (w/v) Triton X-100 (as detergent) in the buffers throughout the purification procedure is essential for maintaining the activity of the enzyme. In the absence of calcium and Triton X-100, the enzyme activity is quickly lost |
| 3.1.1.25 | wild-type-like rePON1-G2E6 and its various mutants are expressed as fusion proteins with thioredoxin and His6 tags |
