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EC Number
Purification (Commentary)
2.5.1.21
-
2.5.1.21
expressed in E. coli
2.5.1.21
gel purification and Ni-NTA column purification
2.5.1.21
HiTrap nickel-chelating column chromatography and MonoQ HR5/5 anion-exchange column chromatography
2.5.1.21
overexpressed in E. coli
2.5.1.21
partial
2.5.1.21
partially purified recombinant enzyme in microsomes is further purified by ultracentrifugation
2.5.1.21
recombinant C-terminally His6-tagged and truncated enzyme from Escherichia coli by cobalt affinity chromatography
2.5.1.21
recombinant enzyme from Escherichia coli strain BL21(DE3)
2.5.1.21
recombinant His-tagged protein found exclusively in inclusion bodies. Solubilization in presence of glycerol
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