EC Number   |
|---|
    2.3.1.30 | - |
| 2.3.1.30 | cells centrifuged, resuspended in 0.01 M Tris-HCl, pH 7.5, sonicated, centrifuged, supernatant subjected to streptomycin sulfate (10%) and ammonium sulfate (70%) precipitation, precipitated protein dissolved in same buffer, desalted on a G-25 column |
| 2.3.1.30 | frozen plant material is homogenized in extraction buffer (50 mM HEPES/KOH, pH 7.4) and centrifuged, supernatant desalted and used for enzyme assays |
| 2.3.1.30 | partially |
| 2.3.1.30 | partially, 3 isozymes |
| 2.3.1.30 | partially, recombinant isozymes SAT-p, SAT-m, and SAT-c from E. coli |
| 2.3.1.30 | protein extraction from frozen, homogenized embryos |
| 2.3.1.30 | recombinant |
| 2.3.1.30 | recombinant C-terminally His-tagged from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
| 2.3.1.30 | recombinant cells are centrifuged, pellet washed with PBS, pH 7.4, resuspended in lysis buffer (50 mM Tris-HCl, pH 8.0), incubated, sonicated, centrifuged, Ni2+ -NTA agarose column, washed with buffer (50 mM Tris-HCl, pH 8.0), eluted imidazole, SDS-PAGE, dialyzed with 50 mM Tris-HCl, pH 8.0, storage with 20% glycerol at -80°C |
