EC Number   |
|---|
    2.3.1.180 | - |
| 2.3.1.180 | 3500fold to homogeneity from leaves in an eight-step procedure including ACP affinity chromatography |
| 2.3.1.180 | immobilized metal ion affinity chromatography |
| 2.3.1.180 | immobilized metal ion affinity chromatography (Ni2+) |
| 2.3.1.180 | native enzyme 147fold from leaves by ammonium sulfate fractionation, ion exchange chromatography, hydrophobic interaction chromatography on a butyl resin, and affinity chromatography, recombinant GST-fusion enzyme from Escherichia coli by glutathione affinity chromatography, GST is cleaved off by thrombin |
| 2.3.1.180 | Ni-NTA column chromatography |
| 2.3.1.180 | Q-Sepharose, MonoQ, and hydroxyapatite column chromatographies (native enzyme) or Ni-NTA agarose column chromatography (selenomethionine-substituted enzyme) |
| 2.3.1.180 | recombinant enzyme from strain JT2038 by ammonium sulfate fractionation, gel filtration, and affinity chromatography |
| 2.3.1.180 | recombinant enzyme from Streptomyces lividans by ammonium sulfate fractionation, gel filtration, ion exchange and hydrophobic interaction chromatography |
| 2.3.1.180 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography |
