EC Number |
---|
1.7.1.6 | - |
1.7.1.6 | 39.3fold by ammonium sulfate fractionation and anion exchange chromatography |
1.7.1.6 | a Cibacron blue agarose 3G column is used |
1.7.1.6 | by anion-exchange chromatography and by gel filtration |
1.7.1.6 | cell extracts of Enterococcus faecalis are prepared, AzoA is purified by hydrophobic, anion exchange and affinity chromatography |
1.7.1.6 | crude cell extracts are applied on a DEAE cellulose column, subsequently to an ammonium sulfate precipitation the enzyme solution is applied on a Butyl Toyopearl 650M column, finally a Sephacryl S200 column is used |
1.7.1.6 | crude cell extracts are applied on a DEAE cellulose column, subsequently to an ammonium sulfate precipitation the enzyme solution is applied on a Phenyl Toyopearl 650M column, finally a Sephacryl S200 column is used |
1.7.1.6 | crude protein extracts from Bacillus velezensis are prepared |
1.7.1.6 | HiPrep 16/10 Q XL anion-exchange column chromatography and HiLoad 26/60 Superdex 200 gel fltration |
1.7.1.6 | His-tagged proteins are purified by gravity-flow chromatography using nickel-nitrilotriacetic acid-agarose |