EC Number |
---|
1.4.3.13 | - |
1.4.3.13 | 46.9fold from aorta by DEAE-cellulose ion exchange chromatography and gel filtration |
1.4.3.13 | affinity, gel-filtration, ion-exchange |
1.4.3.13 | affinity, ion-exchange |
1.4.3.13 | from aorta, to homogeneity, solubilization from connective tissue by 4-6 M urea |
1.4.3.13 | highly purified enzyme peptides |
1.4.3.13 | immobilized metal chelate column chromatography |
1.4.3.13 | lysyl oxidase is a highly insoluble enzyme requiring high concentrations of urea to solubilize. Development and optimization of a method to obtain lysyl oxidase in high yields directly from an Escherichia coli culture without the need for refolding of inclusion bodies using nutrient rich media, overview |
1.4.3.13 | Ni-NTA column chromatography |
1.4.3.13 | nickel affinity column chromatography and ultrafiltration |