EC Number   |
|---|
    1.4.1.4 | - |
| 1.4.1.4 | ammonium sulfate fractionation, Procion Red HE-3B Sepharose CL-6B column chromatography and Q-Sepharose column chromatography |
| 1.4.1.4 | by Cibacron red LS-B dye-affinity purification with a step KCl gradient elution, 17fold purified |
| 1.4.1.4 | by Cibacron red LS-B dye-affinity purification with a step KCl gradient elution, more than 7fold purified to homogeneity |
| 1.4.1.4 | by gel filtration |
| 1.4.1.4 | co-purification with glutathione disulfide reductase |
| 1.4.1.4 | native GDHI' 142fold from cells grown on isophthalate and 510fold from cells grown on 2YT |
| 1.4.1.4 | native isozymes GDHI and GDHII from strain PP4 grown on isophthalate and glucose, respectively. Enzyme yields are better in the presence of 1 mM 2-oxoglutarate, 1 mM EDTA, and 4.35% glycerol. GDHI and GDHII are purified 228fold and 428fold, respectively |
| 1.4.1.4 | of the alpha-subunit, beta-subunit and a truncated subunit missing 40 amino acids at their N-terminus expressed in Escherichia coli |
| 1.4.1.4 | partially |
