EC Number   |
|---|
    1.14.19.1 | - |
| 1.14.19.1 | ammonium sulfate precipitation, gel filtration, ultra filtration, ion-exchange |
| 1.14.19.1 | anionic and nonionic detergents, solubilization and ion-exchange |
| 1.14.19.1 | different fractions by sonication and centrifugation, ion-exchange |
| 1.14.19.1 | ion-exchange |
| 1.14.19.1 | near complete transfer of the in vitro expressed enzyme integral membrane protein into the liposomes, followed by single step purification via centrifugation in a density gradient yielded proteoliposomes with the desaturase in high purity as judged by capillary electrophoresis, method optimization of cell-free translation to the assembly and purification of integral membrane protein complexes, overview |
| 1.14.19.1 | partially |
| 1.14.19.1 | preparation of 11 fractions, ion-exchange, gel filtration |
| 1.14.19.1 | recombinant isozyme SCD1 from Saccharomyces cerevisiae monounsaturated fatty acid auxotroph L8-14C, incorporation of zinc instead of iron into the protein is likely an artifact of protein overexpression, and zinc remains the predominant metal species even when the growth media and purification solutions are supplemented with iron |
| 1.14.19.1 | recombinant isozyme SCD1 partially from transfected HEL-293 cells by microsome preparation |
