EC Number |
---|
1.14.14.1 | CYP102A7 purified by immobilised metal affinity chromatography using the N-terminally attached His6-tag, 6.6fold, ca. 95% pure |
1.14.14.1 | CYP1A2 with NADPH-cytochrome P450 reductase preparation of endoplasmic reticulum membranes from microsomes, formation into lipid vesicles. the lipid composition of purified lipid vesicles affects CYP1A2 substrate metabolism and NADPH-cytochrome P450 reductase-CYP1A2 binding |
1.14.14.1 | gel filtration |
1.14.14.1 | in two steps by nickel chelating column chromatography followed by anion exchange column chromatography |
1.14.14.1 | native enzyme partially by microsome preparation |
1.14.14.1 | Ni-NTA column chromatography, gel filtration |
1.14.14.1 | partial |
1.14.14.1 | partially by microsome preparation |
1.14.14.1 | partially by preparation of subcellular fractions |
1.14.14.1 | Q Sepharose column chromatography and HisTrap HP column chromatography |