EC Number |
---|
1.14.13.25 | - |
1.14.13.25 | 3 components: a soluble CO-binding cytochrome c, a copper-containing protein, another small protein |
1.14.13.25 | component A |
1.14.13.25 | component B of sMMO |
1.14.13.25 | component C |
1.14.13.25 | component D of sMMO recombinant from Escherichia coli as His-tagged thioredoxin-fusion protein, the thioredoxin is cleaved off during purification by factor Xa |
1.14.13.25 | DEAE-Sepharose column chromatography, Q sepharose column chromatography, S-200 gel filtration, and S-300 gel filtration |
1.14.13.25 | hydroxylase component |
1.14.13.25 | hydroxylase component A |
1.14.13.25 | improved purification protocol using stabilizing agents |