EC Number   |
Application |
Reference |
|---|
   6.5.1.2 | analysis |
DNA ligase can be used to determine the ability of other enzymes to act at nicks and gaps in duplex DNA molecules |
1909 |
| 6.5.1.2 | analysis |
DNA ligase can be used to study the primary and secondary structure of DNA molecules |
1909 |
| 6.5.1.2 | analysis |
DNA ligase is an essential reagent in studies on nucleic acid structure and metabolism |
1909 |
| 6.5.1.2 | analysis |
DNA ligase, in combination with polynucleotide kinase, can be used to identify 3'- and 5'-end groups at single-strand interruptions by nearest neighbor analysis |
1909 |
| 6.5.1.2 | analysis |
high-throughput assay for the adenylation reaction of bacterial DNA ligase |
671312 |
| 6.5.1.2 | biotechnology |
LigN is unique amongst DNA ligase enzymes in displaying maximal DNA strand joining activity at above 3 M salt levels. As such the LigN enzyme has potential both as a novel tool for biotechnology and as a model enzyme for studying the adaptation of proteins to high intracellular salt levels |
672808 |
| 6.5.1.2 | medicine |
although NAD+-dependent DNA ligase is essential for mycobacterial viability, only low levels of protein are required for growth. very efficient inhibition of enzyme activity would be required if NAD+-dependent DNA ligase is to be useful as an antibiotic target in mycobacteria |
671380 |
| 6.5.1.2 | medicine |
NAD+-dependent ligases can serve as a valuable target in the development if chemotherapeutics for the treatment of numerous human ailments |
693984 |
| 6.5.1.2 | medicine |
NAD+-dependent ligases can serve as a valuable target in the development of chemotherapeutics for the treatment of numerous human ailments |
-, 693984 |
| 6.5.1.2 | medicine |
potential target for antibiotics |
650967, 652360, 652489 |
