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Search term: molecular biology

Results 1 - 100 of 393 > >>
EC Number
Application
Commentary
glycerol dehydrogenase
molecular biology
enzymatic redox cofactor regeneration in organic media: functionalization and application of recombinant glycerol dehydrogenase and soluble transhydrogenase in reverse micelles, overview
D-xylulose reductase
molecular biology
a high thermostability of PsXDH is obtained by subsequent site-directed mutagenesis of the structural zinc-binding loop. The best mutant in this study (C4/F98R/E101F) shows a 10.8 °C higher thermal transition temperature and 20.8 °C higher half denaturation temperature (T1/2) compared with wild-type
D-lactate dehydrogenase
molecular biology
D-lactate dehydrogenase is useful as a marker gene allowing positive selection of transgenic plants
hydroxymethylglutaryl-CoA reductase (NADPH)
molecular biology
Lactococcus lactis is a potential heterologous host for the production of sesquiterpenes from a herbaceous Malaysian plant, Persicaria minor. A sesquiterpene synthase gene encoding beta-sesquiphellandrene synthase from Persicaria minor is successfully cloned and expressed in Lactococcus lactis. Overexpression of the Lactococcus lactis endogenous 3-hydroxy-3-methylglutaryl Co-A reductase, an established rate-limiting enzyme in the eukaryotic mevalonate pathway, increases the production level of beta-sesquiphellandrene by 1.25-1.60 fold
phosphoglycerate dehydrogenase
molecular biology
results demonstrate that the promoter activity of the human PHGDH gene is positively regulated by the action of transcription factors Sp1 and NF-Y
phosphoglycerate dehydrogenase
molecular biology
results demonstrate that PGDH enhances the levels of betaine by providing the precursor serine for both choline oxidation and glycine methylation pathways
L-gulonolactone oxidase
molecular biology
short-term vitamin A deficiency in broiler chicks reduces GULO activity without concomittant changes in tissue ascorbic acid
galactose oxidase
molecular biology
glycoprotein labeling using engineered variants of galactose oxidase, overview
alcohol oxidase
molecular biology
Pichia pastoris is an efficient host for the expression and secretion of heterologous proteins possessing a strong and tightly regulated promoter from the alcohol oxidase I, AOX1, gene. The transformed cells need to be activated by methanol and grow on methanol as carbon source. With the inducible AOX1 promoter an increase of the copy number above two resulted in a decrease of expression. Combined use of GAP and AOX1 promoters in Pichia pastoris, overview
(S)-2-hydroxy-acid oxidase
molecular biology
the interaction of capsid protein P8 with the GOX of host cells leads to translocation of capsid protein P8 into peroxisomes. The interaction between capsid protein P8 and GOX plays important roles in Rice dwarf phytoreovirus targeting into the replication site of host cells
pyranose dehydrogenase (acceptor)
molecular biology
gene expression analysis using PCR, pdh1 expression is upregulated upon exhaustion of the carbon source and appears to be additionally regulated under conditions of oxygen limitation, PDH production is highest on cellobiose and very low on fructose; gene expression analysis using PCR, pdh2 is constitutively expressed, PDH production is highest on cellobiose and very low on fructose; gene expression analysis using PCR, pdh3 is constitutively expressed, PDH production is highest on cellobiose and very low on fructose
anthocyanidin reductase
molecular biology
a method for the analysis of ANR activity using the detection of coenzyme is established
succinate dehydrogenase
molecular biology
the iron-sulfur subunit (SdhB) of mitochondrial succinate dehydrogenase is encoded by a split and rearranged nuclear gene in Euglena gracilis. The two subgenic modules are transcribed independently and the resulting mRNAs appear to be independently translated, with the two protein products imported into mitochondria. The discovery of this unique molecular marker provides evidence for the monophyly of Euglenozoa that is independent of evolutionary models; the splitting of sdhB in Euglena and trypanosomatids is an example of a unique molecular character that specifically unites these two phylogenetic groups
succinate dehydrogenase
molecular biology
the splitting of sdhB in Euglena and trypanosomatids is an example of a unique molecular character that specifically unites these two phylogenetic groups
succinate dehydrogenase
molecular biology
considering the conservation of amino acids crucial for the SQR activity and the high levels of ROS production from the mitochondrial complex II of the Ascaris suum adult worm together with the absence of complexes III and IV activities in its respiratory chain, it is a good model to examine the reactive oxygen species production from the mitochondrial complex II
succinate dehydrogenase
molecular biology
the relevance of modifications in Alternaria alternata AaSdhB sequence in conferring boscalid resistance is discussed
glutamate dehydrogenase
molecular biology
GDH is essential for the full development of the secretory response in beta-cells
glutamate dehydrogenase
molecular biology
GDH, in conjunction with NADH-glutamte synthase, contributes to the control of leaf glutamate homeostasis, an amino acid that plays a central signaling and metabolic role at the interface of the carbon and nitrogen assimilatory pathways
glutamate dehydrogenase (NADP+)
molecular biology
results implicate glutamate dehydrogenase and NADP-GDH in particular, as a key target of in vivo isophthalate inhibition during ammonium assimilation
glutamate dehydrogenase (NADP+)
molecular biology
promoter of GDH from Xanthophyllomyces dendrorhous is shown to be a valuable tool for controlled gene expression in Basidiomycetes
glutamate synthase (NADPH)
molecular biology
GltA shows 73% identity to the corresponding protein of Oceanobacillus iheyensis; GltB1 shows 74% identity to the corresponding protein of Oceanobacillus iheyensis; GltB2 shows 73% identity to the glutamate synthase of Geobacillus kaustophilus
glutamate synthase (NADPH)
molecular biology
sequence identity between Salmonella typhimurium and Escherichia coli GOGAT is 91%, Salmonella typhimurium GOGAT region can complement an Escherichia coli mutant defective in GOGAT
D-aspartate oxidase
molecular biology
This enzyme is proposed to have a role in the inactivation of the synaptically released D-aspartate. Its C-terminus has a peroxisome targeting signal.
D-octopine dehydrogenase
molecular biology
His-tag-induced crystallization of OcDH is found to be dependent on the length of the His tag
mycothione reductase
molecular biology
a DTNB-coupled assay is developed for time-dependent inhibition of Mycobacterium tubercolosis reductase employing a benzyl glycoside analogue of MSH, from which an efficient mixed disulfide substrate is chemically recycled in situ, thereby greatly reducing the substrate quantities needed for such assays
chlorite O2-lyase
molecular biology
use of the heme enzyme for the rapid, in situ generation of O2 at concentrations far exceeding 2 mM in coupled enzyme reaction systems to study O consumption or O2 involving reaction steps. Catalytic concentrations of chlorite O2-lyase can be used to initiate the reaction of an O2-utilizing (metallo)enzyme by rapid mixing with the highly soluble, non-volatile ClO2, rather than with the sparingly soluble, gaseous O2, e.g. activation of the beta2 subunit of class Ic ribonucleotide reductase from Chlamydia trachomatis by mixing its MnII/FeII complex with ClO2- in the presence of chlorite O2-lyase, overview
Renilla-luciferin 2-monooxygenase
molecular biology
development of a reverse genetic model to characterize the pathway of replication and pathogenesis of the SARS coronavirus. Renilla luciferase is used as a reporter gene and inserted into the backbone of the infectious clone of SARS coronavirus to replace ORF 7a/b (SARS wt-Luc), which is believed to have apoptotic effects on host cells. Recombinant viruses with luciferase constructs are isolated and shown to stably maintain the Renilla luciferase gene and to express subgenomic mRNA encoding luciferase. SARS wt-Luc is a viable virus that allows studies of the effect of subgenomic manipulation on virus efficiacy, both in replication and subgenomic production. This approach offers an alternative to plaque assay analysis in testing the efficiency of anti-SARS agents
Renilla-luciferin 2-monooxygenase
molecular biology
modification of the photoprotein aequorin by attaching selected fluorophores at a unique site on the protein. This will allow for in vitro transfer of bioluminescent energy from aequorin to the fluorophore thus creating an artificial jellyfish. The fluorophores are selected such that the excitation spectrum of the fluorophore overlaps with the emission spectrum of aequorin. By modifying aequorin with different fluorophores, bioluminescent labels with different emission maxima are produced, which will allow for the simultaneous detection of multiple analytes
Renilla-luciferin 2-monooxygenase
molecular biology
dual luciferase enzyme assay system for reporter gene analysis combining both the firefly luciferase enzyme and the Renilla luciferase enzyme in a nonproprietary buffer
Renilla-luciferin 2-monooxygenase
molecular biology
when aequorin is microinjected into cleavage-stage zebrafish embryos, it is largely used up by about 24 hours. Thus, it is not possible to image Ca2+ signals from later stages of zebrafish development using this approach. Transient expression of apoaequorin (i.e., the protein component of aequorin) using aeq-mRNA in zebrafish embryos and then reconstitution of intact aequorin in vivo by loading the coelenterazine cofactor into the embryos separately provides a valuable tool for monitoring Ca2+ signaling during the 24–48 h post fertilization period of zebrafish development. Thus, it effectively extends the aequorin-based Ca2+-imaging window by an additional 24 hours
Renilla-luciferin 2-monooxygenase
molecular biology
the development of variants of Renilla luciferase, which exhibit significantly improved properties compared with the native enzyme, will allow enhanced sensitivity in existing luciferase-based assays as well as enable the development of novel probes labeled with the luciferase protein
Renilla-luciferin 2-monooxygenase
molecular biology
Renilla luciferase, fused to biospecific sequences such as engineered antibodies, can be administered systemically to provide a novel, sensitive method for optical imaging based on expression of cell surface receptors in living organism
Renilla-luciferin 2-monooxygenase
molecular biology
sensitive reproter for studies of gene expression, promoter activity, protein-protein interactions, signal transduction, tumor cell growth, response to therapy
Cypridina-luciferin 2-monooxygenase
molecular biology
construction of a cold-induced expression vector (pCold-ZZ-VL vector) in Escherichia coli cells that results in soluble bioluminescent fusion enzyme with binding ability to monoclonal antibodies, however, the Cypridina luciferase fusion enzyme is soluble but not bioluminescent (in contrast to other luciferases fused to the ZZ-domain of Staphylococcuss aureus protein A)
Cypridina-luciferin 2-monooxygenase
molecular biology
Renilla and Cypridina luciferases should be more appropriate tools for applications requiring the detection of small amounts of substrate as in small molecule detection assays because RLuc and CLuc respond to their luciferin concentration in a linear non-cooperative manner
Oplophorus-luciferin 2-monooxygenase
molecular biology
useful reporter protein in various assay systems including reporter assays and immunoassays
gamma-butyrobetaine dioxygenase
molecular biology
a fluorescence assay based on the detection of fluoride released from reactions of fluorinated substrates with BBOX by the use of tert-butyldimethylsilyl-protected fluorescein is developed. (3S)-3-fluoro-4-(trimethylammonio)butanoate is a good substrate for BBOX, releasing fluoride when subjected to the enzyme
kynurenine 3-monooxygenase
molecular biology
development of a transformant selection system for Tribolium castaneum on the basis of mutant rescue
kynurenine 3-monooxygenase
molecular biology
the wild-type enzyme gene can be used as a marker gene for visually screening transgenic silkworms; wild-type KMO gene can be used as a marker gene for visually screening transgenic silkworms
kynurenine 3-monooxygenase
molecular biology
wild-type KMO gene can be used as a marker gene for visually screening transgenic silkworms
ent-kaurene oxidase
molecular biology
the fungal cytochrome P450 monooxygenase isolated from from Phaeoshaeria sp., strain L487 in the Pichia pastoris expression system might become a tool for functional assays of a variety of rice cytochrome P450 monooxygenases involved in the biosynthesis of secondary metabolites
bacterial luciferase
molecular biology
enzyme can be used to monitor changes in gene expression as a reporter system in slow-growing mycobacteria, i.e. Mycobacterium tuberculosis strain H37Ra, determination of recombinant enzyme decay rate
bacterial luciferase
molecular biology
the enzyme is used as a reporter system tool for analysis of promoter and gene expression activity, overview
phenylalanine 4-monooxygenase
molecular biology
bicistronic expression system is developed which allows the isolation of hybrid forms that exhibit negative interallelic complementation, and may represent a model system for studying the molecular pathogenic mechanisms of PAH gene mutations in compound heterozygous phenylketonuric patients, providing the rationale to understand the observed inconsistencies both in genotype/phenotype correlations and in the response to (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin supplementation
phenylalanine 4-monooxygenase
molecular biology
an automated fluorescence-based continuous real-time PAH activity assay that is faster and more efficient but as precise and accurate as standard methods is developed
betaine-homocysteine S-methyltransferase
molecular biology
osmotic regulation of BHMT may be part of a cell volume-regulatory response and additionally lead to metabolic alterations that depend on the availability of betaine-derived methyl groups
betaine-homocysteine S-methyltransferase
molecular biology
S-adenosylmethionine and 5’-methylthioadenosine down-regulate BHMT expression in HepG2 cells in part by inducing NF-kappaB, which acts as a repressor for the human BHMT gene. While S-adenosylmethionine’s mechanism is NF-kappaB-dependent, 5’-methylthioadenosine has both NF-kappaB-dependent and -independent mechanisms
betaine-homocysteine S-methyltransferase
molecular biology
the BHMT/betaine system directly protects hepatocytes from homocysteine-induced injury but not tunicamycin-induced injury, including an endoplasmic reticulum stress response, lipid accumulation, and cell death
tRNA (uracil54-C5)-methyltransferase
molecular biology
enzyme is used for detecting tRNA-like moieties in viral RNA
methylated-DNA-[protein]-cysteine S-methyltransferase
molecular biology
modest binding cooperativity and high binding densities of AGT are adaptations that allow the enzyme to efficiently search for lesions in the context of chromatin remodeling and DNA replication
protein-L-isoaspartate(D-aspartate) O-methyltransferase
molecular biology
betaine administration of rats prevents the ethanol-induced accumulation of isoaspartyl-containing proteins in the liver by restoring the PIMT-catalyzed protein repair reaction through normalizing the hepatocellular SAM:SAH ratios
glutamate formimidoyltransferase
molecular biology
bifunctional formiminotransferase cyclodeaminase provides a novel marker to study ER-Golgi dynamics
transaldolase
molecular biology
TAL deficiency is shown as a modulator of mitochondrial homoeostasis, Ca2+ fluxing and apoptosis
acetolactate synthase
molecular biology
the gene is useful as a selectable marker for introducing foreign traits into rice when used with pyrimidinylcarboxylate herbicides. The double-mutant W548L/S627I of the ALS gene from rice is not only helpful for introducing useful rice genes into rice by self-cloning as a host-derived selectable marker gene but also can extinguish the scientific concern for antibiotic-resistant genes, leading to minimize public concern for this issue in transgenic plants
acetolactate synthase
molecular biology
the G95A mutation of the ALS gene confers highly specific resistance to pyrimidinyl carboxy herbicides and can be used as a selection marker for transformations
glucosamine-phosphate N-acetyltransferase
molecular biology
an assay for glucosamine-6-phosphate synthase using a yeast glucosamine-6-phosphate N-acetyltransferase 1 (GNA1) as coupling enzyme is developed. The assay measures the production of glucosamine-6-phosphate by either following the consumption of acetyl-CoA spectrophotometrically at 230 nm or quantifying the free thiol with 5,50-dithio-bis(2-nitrobenzoic acid) (Ellman’s reagent) in a discontinuous manner. This method is simple to perform and can be adapted to a 96-well microtiter plate format
glucosamine-phosphate N-acetyltransferase
molecular biology
a microtiter plate based assay to detect the GlmU activity is developed: the assay relies on the enzymes MurA and MurB to convert UDP-GlcNAc into UDP-MurNAc with the concomitant reduction of NADPH. When all enzymes and substrates are present NADPH is oxidized with a concomitant decrease in OD340
arylamine N-acetyltransferase
molecular biology
results show that cisplatin inactivates the NAT1 enzyme by forming an adduct with the catalytic cysteine residue of the enzyme
arylamine N-acetyltransferase
molecular biology
results demonstrate that human P4501A1 and NATs (NAT1 and NAT2) contribute significantly to the activation of PBTA-type compounds to genotoxic metabolites that induce umuC gene expression in Salmonella typhimurium tester strains
dihydrolipoyllysine-residue acetyltransferase
molecular biology
dihydrolipoamide acetyltransferase is shown to be a metabolic longevity factor and is required for calorie restriction-mediated life span extension
octaketide synthase
molecular biology
use of octaketide synthase for rational biosynthetic engineering to generate molecular diversity and pursue innovative, biologically potent compounds
chloramphenicol O-acetyltransferase
molecular biology
the chloramphenicol acetyl transferase gene serves as integration target for the eukaryotic mariner transposon Mos1 regardless of the location (chromosome or plasmid), as tested with in vitro and bacterial transposition assays
chloramphenicol O-acetyltransferase
molecular biology
single-chain variable fragment (scFv) phages are selected with affinity for CAT. Surface plasmon resonance analyses shows that the tested scFv phages have an affinity for CAT with a dissociation constant (Kd) around 1 microM. The selected scFv phages can be used as capture elements in a highly sensitive sandwich ELISA to detect CAT concentration as low as 0.1 ng/ml or 4 pM
chloramphenicol O-acetyltransferase
molecular biology
the enzyme mutant CATA138T may be useful as a genetic marker in Geobacillus spp.
serine O-acetyltransferase
molecular biology
results show that some transgenic plants expressing serine acetyltransferase and cysteine synthase can mitigate detrimental effects of cadmium toxicity, perhaps by efficiently producing and accumulating sulfuric compounds
serine O-acetyltransferase
molecular biology
results show that mitochondria provide the bulk of OAS in the plant cell and are the likely site of flux regulation
homoserine O-acetyltransferase
molecular biology
the HOA gene can be used as a selectable marker for transformation of Gibberella zeae
histone acetyltransferase
molecular biology
the catalytically inactive mutant Y891F is useful for stable binding and purification of unacetylated histone H4 protein
histone acetyltransferase
molecular biology
the results demonstrate the importance of TIPs in the recruitment of p300 to specific promoters and in the regulation of p300 HAT activity through the involvement of the SANT domain
serine C-palmitoyltransferase
molecular biology
results show that SPT modulated programmed cell death plays an important role in the regulation of male gametophyte development of Arabidopsis thaliana
glycylpeptide N-tetradecanoyltransferase
molecular biology
the enzyme can be used for protein N-myristoylation as a tag labeling technique in recombinant expresssion systems. CaNMT is an effective tool for in vitro and in vivo transfer of an azide-modified acid to the N-terminus of a polypeptide derived from a species entirely unrelated to Candida albicans
phospholipid:diacylglycerol acyltransferase
molecular biology
the conversion of a membrane bound lipid metabolizing enzyme into a soluble and active form might be applied to other enzymes with a membrane anchor region.
phosphinothricin acetyltransferase
molecular biology
the bar gene represents a selectable and assayable reporter gene especially suitable for 3’-terminal gene fusions
5,7-dihydroxy-2-methylchromone synthase
molecular biology
use of pentaketide chromone synthase for rational biosynthetic engineering to generate molecular diversity and pursue innovative, biologically potent compounds
polypeptide N-acetylgalactosaminyltransferase
molecular biology
enzyme will be useful for the in vitro glycosylation of proteins obtained from microorganisms by gene manipulation techniques
polypeptide N-acetylgalactosaminyltransferase
molecular biology
overexpression in eukaryotic cell culture leads to inhibition of Activin/Nodal pathway activity in vivo by interference with binding of ActR-IIB to type I TGFbeta receptor proteins that can mediate BMP as well as Nodal signalling
polypeptide N-acetylgalactosaminyltransferase
molecular biology
invariant residue Trp328 is essential for GalNAc-T enzymatic activity, residue Trp316 is important in the interaction with the acceptor polypeptide of GalNAc-T1
polypeptide N-acetylgalactosaminyltransferase
molecular biology
enzyme-linked lectin assay (ELLA) as carbohydrate-binding assay; enzyme-linked lectin assay (ELLA) as carbohydrate-binding assay; enzyme-linked lectin assay (ELLA) as carbohydrate-binding assay; enzyme-linked lectin assay (ELLA) as carbohydrate-binding assay
polypeptide N-acetylgalactosaminyltransferase
molecular biology
polyclonal rabbit anti-GalNAc-T14 IgG (1: 1000 in Western blot, titer: appr. 1: 16000) for study of expression and distribution of human GalNAc-T14
3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase
molecular biology
the stable system using the expression vector pIB/Vf-His-TOPO constitutes an advance for the large scale expression of glycosyltransferases and possibly other glycoproteins in insect cells
ceramide glucosyltransferase
molecular biology
glucosylceramide is essential for MsDef1-mediated growth inhibition of Fusarium graminearum, but not for its pathogenicity
alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
molecular biology
production of rare hybrid oligosaccharides for biochemical and structural studies, 100% conversion of oligosaccharide substrate at room temperature, yield of 42% after purification from reaction mixture
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
molecular biology
beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-p-nitrophenyl and other nitrophenyl-sugar-derivatives are useful as specific inhibitors and as affinity label
peptide-O-fucosyltransferase
molecular biology
O-fucosylation is dispensable for many Notch signaling events during Drosophila development
peptide-O-fucosyltransferase
molecular biology
engineering of an O-fucosylation system in yeast provides a powerful tool for producing proteins with homogenous carbohydrate chains. Such proteins can be used for the analysis of substrate specificity and the production of antibodies that recognize O-glycosylated EGF domains
hypoxanthine phosphoribosyltransferase
molecular biology
the HPRT gene is used as reporter gene in HL-60 cells for investigation of the mutagenic potential of succinyl-acetone by determining the frequencies of somatic mutations in the HPRT reporter gene, overview
hypoxanthine phosphoribosyltransferase
molecular biology
HPRT mutations in vivo in human T-lymphocytes are useful probes for mechanistic investigations, molecular analyses of isolated mutants reveal their underlying mutational changes as well as the T-cell receptor gene rearrangements present in the cells in question, overview
NAD+ ADP-ribosyltransferase
molecular biology
establishment of an immortalized PARP-1-/- murine endothelial cell line HYKO6 as a tool to study PARP-1-mediated endothelial cell dysfunction
dihydropteroate synthase
molecular biology
established coupled enzymatic assay for kinetic analyses of DHPS activity (coupled to pyrophosphate-dependent phosphofructokinase, aldolase, triosephosphate isomerase, alpha-glycerophosphate dehydrogenase) in presence or absence of activity-modulating compounds
rubber cis-polyprenylcistransferase
molecular biology
model for rubber biosynthesis
protein geranylgeranyltransferase type I
molecular biology
the GGTase-I variants with altered substrate specificity can serve as tools for studying GGTase-I substrate selectivity and the effects of prenylation pathway modifications on specific proteins; the GGTase-I variants with altered substrate specificity can serve as tools for studying GGTase-I substrate selectivity and the effects of prenylation pathway modifications on specific proteins
6,7-dimethyl-8-ribityllumazine synthase
molecular biology
outside of the cell, the hollow spherical architecture of the enzyme capsid is used as a template for the encapsulation of cargo proteins, such as green fluorescent proteins, and HIV proteases, and fabrication of uniform layer-by-layer assemblies using non-covalent interactions between surface-displayed His6 and Ni-NTA of enzyme AaLS. The enzyme shows encapsulation capability and surface presentation of ligands, which represent the great potential of AaLS as a versatile delivery vehicle
thymidine kinase
molecular biology
a method to distinguish between the de novo induction of thymidine kinase mutants and the selection of pre-existing thymidine kinase mutants in the mouse lymphoma assay
N-acylmannosamine kinase
molecular biology
GNE-deficient cells, with dramatically increased incorporation of N-acetylmannosamine analogues into glycoproteins, can efficiently be decorated with reactive functional groups, which can be employed in bioorthogonal functionalization strategies for fluorescence labelling or biotinylation
hygromycin B 4-O-kinase
molecular biology
the enzyme can be used as selective marker gene product in production of transgenic plants
hygromycin B 4-O-kinase
molecular biology
the mutant gene hph5 gene can be used as a selection marker in the host-vector system of Thermus thermophilus either on plasmid or by genome integration
hygromycin B 4-O-kinase
molecular biology
Used as marker gene mediating hygromycin resistance.
hygromycin B 4-O-kinase
molecular biology
resistance against hyromycin B mediated by transformation of the hph gene, a selectable marker gen.
hygromycin B 4-O-kinase
molecular biology
hpt gene is used as a selectable marker
hygromycin B 4-O-kinase
molecular biology
hpt gene is used as selectable marker, mediates hygromycin resistance
Results 1 - 100 of 393 > >>