EC Number   |
Recommended Name |
Application |
|---|
   3.4.21.5 | thrombin |
analysis |
development of a biosensor for thrombin detection using surface-enhanced Raman spectroscopy. The method utilizes the electrostatic interaction between capture thrombin aptamer and probe crystal violet molecules. Procedure shows a highly specific selectivity and a linear detection of thrombin in the range from 0.1 nM to 10 nM with a detection limit of about 20 pM and realizes the thrombin detection in human blood serum solution directly |
| 3.4.21.5 | thrombin |
analysis |
development of a direct and an indirect competitive assay for thrombin by an electrochemical aptamer-based assay coupled to magnetic beads. With the direct competitive assay, when the aptamer is immobilised onto the magnetic beads, a detection limit of 430 nM for thrombin is achieved. A detection limit of 175 nM is obtained by detecting the product of the enzymatic reaction catalysed by thrombin. A sandwich assay reaches a detection limit of 0.45 nM of thrombin |
| 3.4.21.5 | thrombin |
analysis |
development of a modified electrochemical sandwich model for target protein detection using differential pulse voltammetry. With model target analyte thrombin, the sensor shows a linear response for thrombin in the range 1-60 nM with a detection limit of 0.5 nM |
| 3.4.21.5 | thrombin |
analysis |
development of an aptamer-based surface enhanced resonance Raman scattering sensor with high sensitivity, specificity, and stability for the detection of human alpha-thrombin. The sensor displays a limit of detection of 100 pM by monitoring the signal change upon the single-step of thrombin binding to immobilized thrombin binding aptamer and specifically discriminates thrombin from other proteins. The sensor can detect 1 nM thrombin in the presence of complex biofluids, such as 10% fetal calf serum, and is sufficiently robust for clinical diagnostic applications |
| 3.4.21.5 | thrombin |
analysis |
study on the effects of dilution on the thrombin generation process in assessing the clotting function. Anticoagulant pathways are far more affected by dilution than the procoagulant pathways. Plasma dilution causes a loss of sensitivity towards thrombomodulin and activated protein C. At dilutions above 1:12 a second wave of prothrombinase-activity is observed |
| 3.4.21.5 | thrombin |
analysis |
design of an aptamer-based suspension array detection platform for the sensitive, specific and rapid detection of human alpha-thrombin as a model. Thrombin is first recognized by a 29-mer biotinylated thrombin-binding aptamer in solution. Then 15-mer TBA modified magnetic beads capture the former aptamer-thrombin to form an aptamer-thrombin-aptamer sandwich complex. The median fluorescence intensity obtained via suspension array technology is positively correlated with the thrombin concentration. The dynamic quantitative working range of the aptamer-based suspension array is 18.37-554.31 nM, and the coefficients of determination R2 are greater than 0.9975. The lowest detection limit of thrombin is 5.4 nM. The method is highly specific for thrombin without being affected by other analogs and interfering proteins. The recoveries of thrombin spiked in diluted human serum are in the range 82.6-114.2% |
| 3.4.21.6 | coagulation factor Xa |
analysis |
development of an assay for the monitoring of anticoagulants inhibiting factor Xa and/or factor IIa. Assay is based on the addition of factor Xa and snake venom RVV-V, i.e. Russell viper venom factor V activator specifically activating factor V and phospholipids to platelet-poor plasma. Assay shows an almost linear dose-response and high sensitivity for unfractionated heparin, low molecular weight heparins, r-hirudin, and argatroban |
| 3.4.21.7 | plasmin |
analysis |
electrochemical assay of plasmin activity based on a ferrocenyl peptide substrate having a plasmin-specific substrate sequence, Lys-thr-Phe-Lys, and immobilized on a gold electrode. Detection limit for plasmin is around 50 ng/ml or 0.15 mU/ml. Ratio of kcat/Km values is 0.063 microM/s |
| 3.4.21.9 | enteropeptidase |
analysis |
cellular libraries of peptide substrates, CLiPS, are used to study substrate specificities, fluorescent reporter substrates on the surface of Escherichia coli as N-terminal conjugates are used as whole-cell protease activity assays |
| 3.4.21.9 | enteropeptidase |
analysis |
enteropeptidase activity is influenced by accessibility of the target site and by downstream sequences |
| 3.4.21.10 | acrosin |
analysis |
the enzyme activity can be used as a reliable predictor of boar sperm freezability |
| 3.4.21.B12 | prostase |
analysis |
enzyme-specific sandwich-type immunoassay using a monoclonal antibody, detection limit is 0.02 microgram per liter, and less than 0.1% cross-reactivity with other human kallikreins |
| 3.4.21.19 | glutamyl endopeptidase |
analysis |
study of structure-function organization of anti-HIV and antitumor proteins MAP30 and GAP31 by limited proteolysis with V8-GSE, enzym may be useful for studying other proteins as well |
| 3.4.21.19 | glutamyl endopeptidase |
analysis |
increase in selectivity of immobilized metal affinity chromatography for phosphopeptides by use of enzyme for protein digestion. Enzyme cleaves proteins at acidic residues and reduces the number of acidic residues in peptides. Application of method to a selction of model proteins and to bovine milk |
| 3.4.21.25 | cucumisin |
analysis |
subcellular localization of protease C1 and pH conditions in protein storage vacuoles in parenchyma cells of seedling cotyledons and maturing seeds examined |
| 3.4.21.36 | pancreatic elastase |
analysis |
elastase digests yield a large proportion of transmembrane peptides facilitating membrane protein identification |
| 3.4.21.50 | lysyl endopeptidase |
analysis |
the enzyme is used for rapid lysis of gram-positive cocci for pulsed-field gel electrophoresis |
| 3.4.21.50 | lysyl endopeptidase |
analysis |
the enzyme is useful in the determination of primary structure of proteins |
| 3.4.21.50 | lysyl endopeptidase |
analysis |
middle-down approach, where the antibody is subjected to limited digestion using the endoproteinase Lys-C, is potentially useful for the accurate, sensitive and routine characterization of recombinant antibodies |
| 3.4.21.50 | lysyl endopeptidase |
analysis |
development of an achromopeptidase lysis procedure for high-volume testing and preparation of samples for PCR detection of methicillin-resistant Staphylococcus aureus. The BD GeneOhm MRSA kit method shows a sensitivity of 92.0%, specificity of 94.6%, positive predictive value of 75.4%, and negative predictive value of 98.5%. The assay is an accurate and rapid test for detection of MRSA colonization |
| 3.4.21.50 | lysyl endopeptidase |
analysis |
method for identification of both the amino and the carboxyl termini of proteins. The method independently uses two proteases, Lys-C and peptidyl-Lys metalloendopeptidase Lys-N, to digest proteins, followed by LC-MS/MS analysis of the two digests. Terminal peptides can be identified as the amino terminal peptide of a protein in Lys-C digest is one lysine residue mass heavier than that in Lys-N digest, the carboxyl terminal peptide in Lys-N digest is one lysine residue mass heavier than that in Lys-C digest, and all internal peptides give exactly the same molecular masses in both the Lys-C and the Lys-N digest, although amino acid sequences of Lys-C and Lys-N peptides are different. Acetylation on N-terminus and protein isoforms, which have different termini, is also determined |
| 3.4.21.50 | lysyl endopeptidase |
analysis |
the enzyme is attractive for the proteolytic generation of peptides to be analyzed by mass spectrometry, usage in quantitative mass spectrometry, overview. It can be used in the presence of protein denaturants which allow better access to cleavage sites and hence better proteolysis. The enzyme has advantages over trypsin, namely, reduced missed cleavage (because KR and RK are cleaved specifically), tolerance to denaturants and requirement for only a single labelled amino acid in experiments using isotopic labelling |
| 3.4.21.50 | lysyl endopeptidase |
analysis |
endoproteinase lysine-C (Lys-C)/trypsin sequential digestion can be used in human 293T cell proteomics sample preparation, evaluation of the advantages of Lys-C/trypsin sequential digestion over trypsin digestion in solution conditions in different aspects, method optimzation, overview |
| 3.4.21.62 | Subtilisin |
analysis |
model for understanding the enormous rate enhancements affected by enzymes |
| 3.4.21.62 | Subtilisin |
analysis |
popular system for protein engineering studies |
| 3.4.21.62 | Subtilisin |
analysis |
analytical on-line liquid chromatography/bioassay with which both chemical and biological information on inhibitory effect of proteins in mixtures can be obtained simultaneously. Assay consists of protein separation using gel filtrationand subsequent homogeneous assay to distinct between active enzyme inhibitors and non-active compounds |
| 3.4.21.62 | Subtilisin |
analysis |
due to high pI-value of enzyme, it does not migrate in the electrophoretic field in the Laemmli buffer system. Fast and simple identification of enzyme by over-running electrophoretic technique with a miniscale culture supernatant |
| 3.4.21.64 | peptidase K |
analysis |
protein engineering approach for increasing activity and heat stability of proteinase K. Protein design algorithms that only require the testing of a small number of variants represent a significant step towards a generic, resource-optimized protein engineering process |
| 3.4.21.64 | peptidase K |
analysis |
nonspecific protease proteinase K can be used as an alternative to trypsin for cross-linking studies, digestion by proteinase K results in a family of related cross-linked peptides, all of which contained the same cross-linking sites, thus providing additional verification of the cross-linking results. Using proteinase K, the affinity-purifiable CID-cleavable and isotopically coded cross-linker cyanurbiotindipropionylsuccinimide and MALDI-MS cross-links are found for all of the possible cross-linking sites of native and oligomeric forms of prion protein substrates, overview. After digestion with proteinase K, the mass distribution of the crosslinked peptides is very suitable for MALDI-MS analysis |
| 3.4.21.64 | peptidase K |
analysis |
improvement of RNA extraction from papillary cancer-derived K1 cells and thyroid fine-needle aspiration biopsy (FNAB) specimens suspended in liquid-based cytology (LBC) solutions during fine-needle aspiration biopsy diagnostics of thyroid diseases. Commercial proteinase K treatment is essential for efficient RNA extraction from the fixed cells. Proteinase K treatment facilitates RNA recovery from rigid cells after dehydration. RNA molecules are largely released from the fixed cells even after 1-h treatment. U6 small nuclear RNA was detected in these RNA samples by reverse transcription-PCR. Method overview |
| 3.4.21.66 | Thermitase |
analysis |
use of enzyme for generation of peptides for mass spectrometric characterization |
| 3.4.21.72 | IgA-specific serine endopeptidase |
analysis |
the ability of IgA1 protease to cleave exclusively IgA1 without affecting IgA2 molecules allows a more economical and reliable method for determination of these antibody subclasses in human sera based on recombinant Neisseria meningitidis IgA1 protease. Estimation of IgA1 and IgA2 content in human serum and of IgA subclass levels and IgA1/IgA2 ratio using recombinant active and inactive forms of IgA1 protease from Neisseria meningitidis, method evaluation, overview |
| 3.4.21.73 | u-Plasminogen activator |
analysis |
assay of cellular internalization and localization of enzyme:PAI-2 inhibitor complex based on the use of inhibitor labelled with Alexa488 fluorochrome and a polyclonal antibody |
| 3.4.21.73 | u-Plasminogen activator |
analysis |
optical zymography technique that specifically detects enzyme activity in biological samples via fluorescence emission at 695 nm. Method can efficiently distinguish the active two-chain enzyme from its proenzyme and directly measure enzyme activities in different cancer cell lines |
| 3.4.21.73 | u-Plasminogen activator |
analysis |
quantification of uPA and inhibitor PAI-1 mRNA expression in breast cancer cell lines as well as in tumor tissue of breast cancer patients by sensitive quantitative real-time PCR assays, based on the LightCycler technology. In breast cancer cell lines, mRNA and antigen values are highly correlated for both uPA and PAI-1 I. Correlations between uPA/PAI-1 mRNA and protein in the breast cancer samples were found to be distinctly weaker or not significant. Quantitative determination of mRNA expression for both factors does not mirror antigen levels in breast cancer tissue |
| 3.4.21.74 | venombin A |
analysis |
the enzyme may be useful for developing potential diagnostic agents for detecting coagulation disorders |
| 3.4.21.75 | Furin |
analysis |
bioluminogenic probes can specifically image furin activity in xenografted breast cancer tumors in mice |
| 3.4.21.75 | Furin |
analysis |
sensitive and specific assay for furin activity using an antibody capture step to immobilise furin from whole cell lysates. The assay has a minimum detection limit of 0.006 nM and is sensitive enough to determine the furin activity of many of the cell lines tested |
| 3.4.21.76 | Myeloblastin |
analysis |
knowledge of the protonation states of the ionizable residues in an enzyme like PR3 is a prerequisite to an accurate description of its structure and mechanism |
| 3.4.21.77 | semenogelase |
analysis |
development and employment of counter-SELEX (systematic evolution of ligands by exponential enrichment) procedures to identify specific RNA aptamers against the purified active PSA, wich is not only a specific marker but also a target molecule for diagnosis and therapy of prostate cancer. The aptamers have a specific binding activity against the active PSA, but not for GST or proPSA |
| 3.4.21.77 | semenogelase |
analysis |
development of a simultaneous electrochemical biosensor, using the goldmodified screen-printed carbon dual sensor, for free and total PSA for monitoring PSA production from three different cultures of human androgen-sensitive prostate tumor cells |
| 3.4.21.77 | semenogelase |
analysis |
development of an electrical immunosensor for the detection of PSA using a microgapped electrode array based on enzymatic silver deposition. This electrical immunosensor exhibits a linear response with PSA concentrations over a 6-decade range from 1.0 pg/l to 1.0 microg/l, with detection limit of 0.9 pg/l. PSA concentrations using this immunosensor agree within 10% of those obtained using a commercial chemiluminescent immunoassay |
| 3.4.21.77 | semenogelase |
analysis |
mass spectrometry annotation can identify more molecular forms of PSA compared with Western and zymographic analyses. Observation of various isoforms of PSA in patients may contribute to the further identification of disease-relevant heterogeneity of PSA, including transcriptional and post-translational modifications present due to various stages and causes of prostate disease |
| 3.4.21.77 | semenogelase |
analysis |
development of PSA and Fab anti-PSA biosensor arrays using UV light-assisted molecular immobilization LAMI, aiming at the detection and quantification of PSA, as a cancer marker. The technology involves formation of free, reactive thiol groups upon UV excitation of protein aromatic residues located in spatial proximity of disulfide bridges, conserved in both PSA and Fab molecules. The thiol groups bind onto thiol reactive surfaces leading to oriented covalent protein immobilization. LAMI technology is successful in immobilizing biomedically relevant molecules while preserving their activity |
| 3.4.21.77 | semenogelase |
analysis |
real-time detection of prostate-specific antigen PSA in diluted human serum without labeling by use of an amplitude-sensitive paired surface plasma wave biosensor PSPWB. The detection limit of PSPWB is 8.4 Ć 10-9 refractive index units, and the PSPWB can measure PSA in a phosphate buffered saline solution from 10 fg/ml to 100 pg/ml, i.e. about 3 pM, successfully, with a linear relationship between PSA concentrations and surface plasmon resonance signals. The PSPWB successfully detects PSA in diluted human serum as well |
| 3.4.21.79 | granzyme B |
analysis |
caution in the design and interpretation of experiments using GrBs from different species due to distinct tetrapeptide specificities and abilities to recruit the BID pathway |
| 3.4.21.84 | limulus clotting factor C |
analysis |
recombinant factor C assay for measuring endotoxin in house dust: comparison with LAL, and (1->3)-beta-D-glucans. In the Limulus amebocyte lysate assay endotoxin is detected through a reaction cascade, initiated by the binding of endotoxins to Factor C. The advantage of an assay using the genetically engineered factor Cbar in measuring endotoxins is that recombinant factor Cbar does not contain factor G that can produce interference from (1->3)-beta-D-glucans |
| 3.4.21.86 | Limulus clotting enzyme |
analysis |
screen-printed endotoxin sensor based on amperometric detection of protease activity. The amperometric measurement with a screen-printed electrode provides compact, low-cost, and easy-to-use sensors for on-site monitoring of endotoxin |
| 3.4.21.89 | Signal peptidase I |
analysis |
development of a fluorescence resonance energy transfer-based assay method as a rapid and reliable tool in future research for the identification and validation of potential SPase I inhibitors |
| 3.4.21.91 | Flavivirin |
analysis |
NS2B-NS3pro K48A mutant construct is well suited for follow-up purification and structural and drug design studies |
| 3.4.21.91 | Flavivirin |
analysis |
two sets of prediction approaches. Secondary structure prediction performed using available structure prediction servers. A second approach makes use of the information on the secondary structures extracted from structure prediction servers, threading techniques and DSSP database of some of the templates used in the threading techniques. Consensus on the one-dimensional secondary structure of Dengue virus type 2 protease obtained from each approach and evaluated against data from the recently crystallised Dengue virus type 2 NS2B/NS3 obtained from the Protein Data Bank. The second approach shows higher accuracy compared to the use of prediction servers only and thus is applicable to the initial stage of structural studies of proteins with low amino acid sequence homology against other available proteins in the Protein Data Bank |
| 3.4.21.92 | Endopeptidase Clp |
analysis |
development of a ClpXP protein degradation systemusing purified ClpXP in a cell-free transcription-translation system |
| 3.4.21.93 | Proprotein convertase 1 |
analysis |
highly specific and potent PC1 inhibitors proSAAS-(235-246) and proSAAS-(235-244) may be useful in development of an effective affinity procedure for the purification of PC1 |
| 3.4.21.97 | assemblin |
analysis |
cell growth selection system in the yeast Saccharomyces cerevisiae that can detect and characterize HCMV protease activity and is applicable to screen for HCMV protease inhibitors in a high-throughput format |
| 3.4.21.98 | hepacivirin |
analysis |
generation of NS3/4A/Lap/LC-1 triple transgenic mouse liver-specifically and conditionally expressing reporter luciferase Fluc, Cre recombinase and reverse tetracycline-controlled transcriptional activator. NS3/4A protein is strictly and conditionally expressed in the liver of doxycycline-induced triple transgenic mice |
| 3.4.21.105 | rhomboid protease |
analysis |
screening assay using fluorescence polarization activity-based protein profiling |
| 3.4.21.118 | kallikrein 8 |
analysis |
binding assessment of ligands to kallikrein-8 using a residue-wise decomposition of the binding energy. Use of chemical energy-wise decomposition or CHEWD for residue-wise decomposition of binding energies |
| 3.4.21.119 | kallikrein 13 |
analysis |
substrate substrate ABZ-Val-Arg-Phe-Arg-Ser-Thr-Gln-Tyr(3-NO2)-NH2 is suitable for determination of KLK13 activity and is successfully converted into an activity-based probe by the incorporation of a chloromethylketone warhead and biotin bait. The modified subvsrtae is applicable in complex biological samples |
| 3.4.21.122 | transmembrane protease serine 2 |
analysis |
development of a cell-based fusion assay for the S protein in a TMPRSS2-dependent manner using cell lines expressing Renilla luciferase-based split reporter proteins. S-protein is stably expressed in the effector cells, and the corresponding receptor for S, CD26, is stably coexpressed with TMPRSS2 in the target cells. Membrane fusion between the effector and target cells is quantitatively measured by determining the Renilla luciferase activity. The assay is optimized for a 384-well format |
| 3.4.21.122 | transmembrane protease serine 2 |
analysis |
use of TMPRSS2 to produce high titre influenza hemagglutixadnin lentiviral pseudotypes from Group 2 influenza viruses. These lentiviral pseudotypes transduce HEK293T/17 cells with high efficiency. The Group 2 influenza pseudotype particles can be used as surrogate antigens in neutralization assays and are efficiently neutralized by corresponding influenza virus reference sera |
| 3.4.22.2 | papain |
analysis |
use of the enzyme in sequence determination |
| 3.4.22.2 | papain |
analysis |
study on the cross-sectional distribution of methylene blue and papain in porous silicon layers by TOF-SIMS. The larger Papain molecule distributes itself in a similar manner to methylene blue demonstrating larger molecules can be effectively incorporated into such pore structures |
| 3.4.22.2 | papain |
analysis |
study on the mixed micellar behavior of anionic surfactant, sodium dodecylsulfate, and cationic surfactant, dodecylethyldimethylammonium bromide, in aqueous solution of papain. The effect of concentration of papain on mixed micellar behavior indicates that with increase in the concentration of protein, the critical aggregation concentration and critical micellizationconcentration values increase. The unfolding of the polypeptide chain in the presence of mixed surfactant has been observed |
| 3.4.22.3 | ficain |
analysis |
use for antibody screening of blood donor samples with the microplate system autoanalyzer |
| 3.4.22.3 | ficain |
analysis |
the enzyme should prove generally useful in peptide sequencing |
| 3.4.22.56 | caspase-3 |
analysis |
cell-permeable substrate N-acetyl-L-Asp-L-Glu-L-Val-L-Asp-NĀ-morpholinecarbonyl-rhodamine 110, high turnover rate and sensitivity both in enzyme solution and in living cells |
| 3.4.22.56 | caspase-3 |
analysis |
the highly cell-permeable caspase-3 substrate is obtained by linking a fluorogenic DNA-binding dye to the caspase-3 recognition sequence that renders the dye nonfunctional. On substrate cleavage, the dye is released and becomes highly fluorescent on binding to DNA. DEVD-NucView488 detects caspase-3 activation within a live-cell population much earlier and with higher sensitivity compared with other apoptosis reagents. Cells incubated with DEVD-NucView488 exhibit no toxicity and normal apoptotic progression. DEVD-NucView488 is an ideal substrate for kinetic studies of caspase-3 activation because it detects caspase-3 activity in real-time and also efficiently labels DNA in nuclei of caspase-3-activated cells for real-time fluorescent visualization of apoptotic morphology |
| 3.4.22.69 | SARS coronavirus main proteinase |
analysis |
developing a novel red-shifted fluorescence-based assay for 3CLpro and its application for identifying small-molecule anti-SARS agents from marine organisms |
| 3.4.22.69 | SARS coronavirus main proteinase |
analysis |
development of a dual-color probe for the simultaneous detection of Mpro and PLpro by FRET in vitro and in cells. The probe produces fluorescence from both the Cy3 and Cy5 fluorophores that are cleaved by Mpro and PLpro |
| 3.4.22.69 | SARS coronavirus main proteinase |
analysis |
FRET-based method to assess the proteolytic activity of SARS-CoV-2 3CLpro using intramolecularly quenched fluorogenic peptide substrates corresponding to the cleavage sequence of SARS-CoV-2 3CLpro |
| 3.4.22.69 | SARS coronavirus main proteinase |
analysis |
quantitative structure-activity relationship model, molecular docking studies and molecular dynamics simulation to identify inhibitors, the model is applied to three large databases and reports top 25 compounds from each database |
| 3.4.22.69 | SARS coronavirus main proteinase |
analysis |
SARS-CoV-2-infected Vero E6 cell viability assay for detection of antiviral activity |
| 3.4.22.70 | sortase A |
analysis |
development of a reverse-phase HPLC assay to identify and characterize sortase reaction intermediates |
| 3.4.22.70 | sortase A |
analysis |
semisynthetic active site mutant enzymes containing selenocysteine and homocysteine might represent useful tools for further biochemical investigations and engineering approaches of sortases A |
| 3.4.22.71 | sortase B |
analysis |
method for sequence specificity determination of sortases or other bond-forming enzymes that recognize an amino acid sequence. Using mixtures of recognition peptides of limited complexity, which are reacted with biotinylated substrates, the biotinylated transpeptidation products are isolated with magnetic streptavidin beads and analyzed via liquid chromatography and mass spectrometry |
| 3.4.22.B79 | nsP2 protease |
analysis |
assay for quantitative measurement of Nsp2 protease activity based on a substrate fusion protein consisting of eGFP and Gaussia luciferase linked together by a small peptide containing a Nsp2 protease cleavage sequence. The expression of the substrate protein in cells along with recombinant Nsp2 protease results in cleavage of the substrate protein resulting in extracellular release of free Gaussia luciferase |
| 3.4.22.B79 | nsP2 protease |
analysis |
nsP2 protease-based cell free high throughput screening assay |
| 3.4.22.B80 | SARS-CoV papain-like protease |
analysis |
development of a dual-color probe for the simultaneous detection of Mpro and PLpro by FRET in vitro and in cells. The probe produces fluorescence from both the Cy3 and Cy5 fluorophores that are cleaved by Mpro and PLpro |
| 3.4.23.1 | pepsin A |
analysis |
combined use of a theoretical model that relates electrophoretic behaviour of peptides to their sequence together with capillary electrophoresis-mass spectrometry to characterize the cleavage specificity of recombinant enzymes. Characterization of a protein lysate using recombinant and natural pepsin |
| 3.4.23.B1 | napsin |
analysis |
enzyme is a specific marker in diagnosis of primary lung adenocarcinoma and to distinguish it from metastatic adenocarcinoma |
| 3.4.23.B1 | napsin |
analysis |
identification of suitable fluorogenic protease substrates for the pharmaceutically important napsin A by an easy, solid-phase combinatorial assay technology |
| 3.4.23.B5 | murine leukemia virus protease |
analysis |
development of an in vitro enzymatic assay system to characterize XMRV protease-mediated cleavage of host-cell proteins |
| 3.4.23.B10 | Rous sarcoma virus retropepsin |
analysis |
coexpression of human prorenin receptor hPRR and RSV protease domain-deleted mutant in silkworm larvae results in display of human prorenin receptor on the surface of RSV virus-like particles in an active form. This transmembrane protein display system using RSV Gag in silkworm larvae is applicable to expression of intact transmembrane proteins and binding assays of transmembrane proteins to its ligands |
| 3.4.23.B10 | Rous sarcoma virus retropepsin |
analysis |
development of a rapid immunochromatographic strip for detecting capsid protein antigen p27 of avian leukosis virus. The test strip can detect 600 pg purified recombinant p27 protein but also quantifies avian leukosis virus as low as 70 TCID50, which is comparable to the commercial enzyme-linked immunosorbent assay |
| 3.4.23.B14 | plasmepsin IV |
analysis |
PSM4 is unusable for structural studies by hydrogen/deuterium exchange coupled to mass spectrometry, DXMS, since the method requires the use of proteases working at acidic pH and low temperatures, in contrast to PSM2, EC 3.4.23.39 |
| 3.4.23.16 | HIV-1 retropepsin |
analysis |
method for picomolar electrochemical detection of human immunodeficiency virus type-1 protease using ferrocene-pepstatin-modified surfaces studied by cyclic voltammetry and electrochemical impedance spectroscopy. Both gold nanoparticles and thiolated single walled carbon nanotubes/gold nanoparticles electrode materials show enhanced electrochemical responses to increasing concentrations of HIV-1 protease with shifting to higher potentials as well as decrease in the overall signal intensity. The sensing electrode modified with thiolated ingle walled carbon nanotubes/gold nanoparticles shows an estimated detection limit of 0.8 pM |
| 3.4.23.B24 | signal peptide peptidase |
analysis |
application of the photophore walking technique for probing the active sites of SPP. Nontransition state gamma-secretase inhibitors inhibit labeling of gamma-secretase by activity-based probes but enhance labeling of SPP. The opposite is true of gamma secretase modulators, which have little effect on the labeling of gamma-secretase but diminish labeling of SPP |
| 3.4.23.39 | plasmepsin II |
analysis |
cheap and high-throughput heterogeneous enzymatic assay for measuring plasmepsin II activity in order to use it as a tool in the discovery of new inhibitors of this enzyme |
| 3.4.23.46 | memapsin 2 |
analysis |
development of robust assay for cerebrospinal fluid beta-secretase-1 activity with sensitivity down to 1 pM of recombinant enzyme |
| 3.4.23.46 | memapsin 2 |
analysis |
identification of potential inhibitors of beta-secretase using in silico multi-filter techniques, substructure screening, computer-aided ligand docking, binding free energy calculations, and partial interaction energy analyses. Method retrieves all known inhibitors from the compound database investigated |
| 3.4.23.46 | memapsin 2 |
analysis |
development and validation of novel plasma BACE activity, soluble amyloid precursor proteins alpha and beta assays. Plasma BACE activity assay is sensitive and specific |
| 3.4.23.48 | plasminogen activator Pla |
analysis |
multiplex real-time polymerase chain reaction assay for the detection of Yersinia pestis and Yersinia pseudotuberculosis using the Yersinia pestis pla gene coding for plasminogen activator and the ypo2088 gene. Assays are both sensitive and specific, the lower detection limit is 10-100 fg of extracted total DNA |
| 3.4.23.48 | plasminogen activator Pla |
analysis |
development of a highly sensitive one-step PLA-enzyme immunoassay and an immunostrip, usable as a rapid test under field conditions. The PLA-immunometric tests allow a rapid and easy detection of Yersinia pestis in environmental and flea samples |
| 3.4.24.B4 | matrix metalloproteinase-13 |
analysis |
MMP-13 expression might be an indicator for increased extracellular matrix remodeling and early signs of vertebral compression in farmed Atlantic salmon |
| 3.4.24.B4 | matrix metalloproteinase-13 |
analysis |
generation of a neutralizing antibody specifc for the active form of MMP-13, but not for the latent form, or other MMPs. antibody can be used to measure active MMP-13 selectively by an enzyme-linked immunosorbet assay. The antibody suppresses the cleavage of type II collagen in human articular chondrocyte cultures, and is thought to inhibit MMP-13 activity effectively |
| 3.4.24.7 | interstitial collagenase |
analysis |
detection of localized extracellular sites of protease activity by use of fluorescent biosensor rhodamine 6G-labeled KDP-6-aminohexanoic acid-GPLGIAGIG-6-aminohexanoic acid-PKGY. Protease activity is localized at the polarized leading edge of migrating tumor cells rather than further back on the cell body. The path of proteolytic cleavage by a migrating cell can be visualized in 2- and 3-dimensional matrices. Probe can be used to determine inhibitor concentrations needed to suppress cell-surface protease activity |
| 3.4.24.7 | interstitial collagenase |
analysis |
generation of specific recombinant human monoclonal antibody SP1, which may serve as building block for the development of antibody-based therapy strategies in mouse models of pathology |
| 3.4.24.11 | neprilysin |
analysis |
the synthetic fluorogenic peptide substrate qf-Abeta(12-16)AAC is more sensitive to NEP than the previously reported peptide substrates, so that concentrations of NEP as low as 0.03 nM can be detected at peptide concentration of 0.002 mM. It can be used for high-throughput screening of compounds that upregulate NEP |
| 3.4.24.17 | stromelysin 1 |
analysis |
generation of specific recombinant human monoclonal antibody SP3, which may serve as building block for the development of antibody-based therapy strategies in mouse models of pathology |
| 3.4.24.17 | stromelysin 1 |
analysis |
unspecific staining in tissue sections of both animal strains with commercial anti-MMP-3 antibody JM3523 and positive but enzyme unspecific staining with the anti-MMP-3 antibody MAB 548 in both MMP-3 wild-type and MMP-3-deficient mouse skin wounds |
