EC Number   |
Organic Solvent |
Reference |
|---|
   3.4.21.1 | acetonitrile |
approx. 50% loss of activity after 20 min at 25°C in 70% acetonitrile, complete loss of activity after 20 min at 25°C in 75% acetonitrile, approx. 40% loss of activity after 20 min at 25°C in 95% acetonitrile |
649560 |
| 3.4.21.1 | dimethyl sulfoxide |
study on the influence of different water-dimethyl sulfoxide mixtures encapsulated in 1,4-bis-2-ethylhexylsulfosuccinate/n-heptane reverse micelles on the enzymatic hydrolysis of N-benzoyl-L-tyrosine p-nitroanilide by alpha-chymotrypsin. The enzyme dissolved in a 20% molar ratio of the dimethyl sulfoxide-water mixture does not present enzymatic activity. There is preferential solvation of the 1,4-bis-2-ethylhexylsulfosuccinate reverse micelle interface by water molecules. The kinetic parameters are determined at fixed ratio ([water] + [dimethyl sulfoxide])/[1,4-bis-2-ethylhexylsulfosuccinate] = 20 at different dimethyl sulfoxide-water compositions. Michaelis-Menten mechanism is valid for alpha-chymotrypsin in all the reverse micelle systems studied and the reaction takes place at the reverse micelle interface. The enzyme encapsulated by the reverse micelles shows catalytic effects with similar kcat/KM values at any dimethyl sulfoxide composition investigated |
732430 |
| 3.4.21.1 | dioxane |
approx. 50% loss of activity after 20 min at 25°C in 20% 1,4-dioxane, complete loss of activity after 20 min at 25°C in 60% 1,4-dioxane, approx. 80% loss of activity after 20 min at 25°C in 95% 1,4-dioxane |
649560 |
| 3.4.21.1 | Ethanol |
almost complete loss of activity after 10 min in 60% ethanol, no decrease in activity in the presence of 1.2 M CaCl2 |
649617 |
| 3.4.21.1 | Ethanol |
approx. 50% loss of activity after 20 min at 25°C in 20% ethanol, complete loss of activity after 20 min at 25°C in 40% ethanol, approx. 60% loss of activity after 20 min at 25°C in 95% ethanol |
649560 |
| 3.4.21.1 | Ethanol |
approx. 50% loss of activity after 5 min at 25°C in 60% ethanol, almost complete loss of activity after 10 min, approx. 50% loss of activity after 10 min at 25°C in 50% ethanol, approx. 50% loss of activity after 15 min at 25°C in 40% ethanol, approx. 30% loss of activity after 40 min at 25°C in 30% ethanol, no loss of activity after 120 min at 25°C in 60% ethanol in the presence of 100 mg/ml polyethylene glycol 2000, approx. 50% loss of activity after 120 min at 25°C in 60% ethanol in the presence of 1.4 M D-fructose, 1.24 M D-sorbitol, 700 mM saccharose, or 12.5% glycerol |
649596 |
| 3.4.21.1 | Ethanol |
approx. 60% loss of activity after 20 min in 40% ethanol, complete loss of activity after 20 min in 60% ethanol, 70% loss of activity after 60 min in 60% ethanol in the presence of polyethylene glycol 6000, 12000 or 20000 |
649638 |
| 3.4.21.1 | Ethanol |
at 50% ethanol alpha-chymotrypsin has an ordinary beta-sheet structure, at 70% or 90% ethanol the structure changes to a native-like, i.e. alpha and beta type, structure |
650617 |
| 3.4.21.1 | more |
effect of cationic surfactants cetyltriphenylphosphonium bromide, cetyltributylphosphonium bromide and cetyltrimethylammonium bromide on the hydrolysis of 4-nitrophenyl benzoate catalyzed by alpha-chymotrypsin. The ester is hydrolyzed readily in all the surfactants with the highest activity shown in cetyltributylphosphonium bromide |
732428 |
| 3.4.21.1 | more |
hydrolysis of 4-nitrophenyl acetate catalysed by alpha-chymotrypsin in sodium 1,4-bis(2-ethylhexyl)sulfosuccinate/isooctane/buffer reverse micelles. The increase in alpha-chymotrypsin activity and stability is an optimum at [H2O]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 10, and [isooctane]/[sodium 1,4-bis(2-ethylhexyl)sulfosuccinate] = 5 |
732228 |
