EC Number   |
General Stability |
Reference |
|---|
    1.7.3.3 | a dextrin-uricase conjugate is more resistant to simulated physiological conditions and trypsin |
764861 |
| 1.7.3.3 | after modification with monomethoxy-poly(ethylene glycol)-5000, the recombinant intracellular uricase shows residual activity of about 65% |
711515 |
| 1.7.3.3 | ammonium sulfate protects from inactivation at low pH |
394173 |
| 1.7.3.3 | at 37°C, pH 7.4 half-life is about 3 weeks |
741750 |
| 1.7.3.3 | at 37°C, pH 9.2 half-life is about 6 weeks |
741750 |
| 1.7.3.3 | at the physiological pH, significant increase of enzyme activity is found for the uricase entrapped in the lipid vesicles (1.8times that of free uricase) at their respective optimum pH. Free uricase shows rapid decrease in its enzymatic activity with a half life of less than 20 min when incubated with trypsin. Uricase entrapped in the lipid vesicles gradually loses its activity but still 50% of the original activity remains after 60 min (remaining activity is 7.32% in case of free uricase) |
712197 |
| 1.7.3.3 | borate stabilizes |
394162 |
| 1.7.3.3 | conjugated uricases are more stable to trypsin digestion |
394185 |
| 1.7.3.3 | Cu2+ inactivates at low temperature, uric acid prevents inactivation |
394173 |
| 1.7.3.3 | dithiothreitol effect of treatment with dithiothreitol on extraction and purification |
394164 |
