1.13.12.7	firefly luciferase	hydrolysis	-	Photinus pyralis	P08659	
2.3.1.164	isopenicillin-N N-acyltransferase	hydrolysis	-	Penicillium chrysogenum	-	
2.3.3.16	citrate synthase (unknown stereospecificity)	hydrolysis	of intermediate citryl-CoA	Sus scrofa	-	
2.3.3.16	citrate synthase (unknown stereospecificity)	hydrolysis	of intermediate citryl-CoA	Thermoplasma acidophilum	-	
2.3.3.21	(R)-citramalate synthase	hydrolysis	-	Leptospira interrogans	-	
2.4.1.9	inulosucrase	hydrolysis	-	Limosilactobacillus reuteri	-	
2.7.8.8	CDP-diacylglycerol-serine O-phosphatidyltransferase	hydrolysis	-	Escherichia coli	P23830	
3.1.1.1	carboxylesterase	hydrolysis	-	Homo sapiens	-	
3.1.1.10	tropinesterase	hydrolysis	-	Oryctolagus cuniculus	-	
3.1.1.14	chlorophyllase	hydrolysis	-	Arabidopsis thaliana	O22527	
3.1.1.14	chlorophyllase	hydrolysis	-	Ginkgo biloba	Q7Y0K5, Q7Y0K5	
3.1.1.14	chlorophyllase	hydrolysis	-	Musa cavendishii	-	
3.1.1.14	chlorophyllase	hydrolysis	-	Pachira macrocarpa	-	
3.1.1.14	chlorophyllase	hydrolysis	-	Phaeodactylum tricornutum	-	
3.1.1.14	chlorophyllase	hydrolysis	-	Triticum aestivum	-	
3.1.1.23	acylglycerol lipase	hydrolysis	-	Homo sapiens	Q99685	
3.1.1.3	triacylglycerol lipase	hydrolysis	-	Moesziomyces antarcticus	-	
3.1.1.3	triacylglycerol lipase	hydrolysis	-	Rhizomucor miehei	-	
3.1.1.3	triacylglycerol lipase	hydrolysis	hydrolysis of glycerol ester bonds occurs non-specifically	Amycolatopsis mediterranei	-	
3.1.1.31	6-phosphogluconolactonase	hydrolysis	-	Trypanosoma brucei	Q385D6	
3.1.1.43	alpha-amino-acid esterase	hydrolysis	-	Acetobacter pasteurianus	Q8VRK8	
3.1.1.47	1-alkyl-2-acetylglycerophosphocholine esterase	hydrolysis	-	Homo sapiens	-	
3.1.1.47	1-alkyl-2-acetylglycerophosphocholine esterase	hydrolysis	-	Mammalia	-	
3.1.1.47	1-alkyl-2-acetylglycerophosphocholine esterase	hydrolysis	-	Rhodnius prolixus	-	
3.1.1.47	1-alkyl-2-acetylglycerophosphocholine esterase	hydrolysis	-	Schizosaccharomyces pombe	-	
3.1.1.47	1-alkyl-2-acetylglycerophosphocholine esterase	hydrolysis	hydrolysis of the sn-2 fatty acyl, ester, bond of phospholipids	Homo sapiens	-	
3.1.1.74	cutinase	hydrolysis	-	Pseudomonas putida	-	
3.1.1.76	poly(3-hydroxyoctanoate) depolymerase	hydrolysis	-	Pseudomonas putida	-	
3.1.1.84	cocaine esterase	hydrolysis	-	Rhodococcus sp.	Q9L9D7	
3.1.13.1	exoribonuclease II	hydrolysis	-	Escherichia coli	-	
3.1.13.1	exoribonuclease II	hydrolysis	-	Pseudomonas putida	Q88DE8	
3.1.2.25	phenylacetyl-CoA hydrolase	hydrolysis	-	Thauera aromatica	-	
3.1.2.6	hydroxyacylglutathione hydrolase	hydrolysis	-	Glycine max	-	
3.1.26.3	ribonuclease III	hydrolysis	-	Saccharomyces cerevisiae	-	
3.1.3.32	polynucleotide 3'-phosphatase	hydrolysis	although unable to dephosphorylate a 3'-PO4-terminated single strand, the enzyme is equally adept at removing the 3'-PO4 from an internal nick or gap in duplex DNA, a recessed 3' end of a 5'-tailed primer-template, or a blunt DNA duplex with no single-strand tail	Saccharomyces cerevisiae	-	
3.1.3.32	polynucleotide 3'-phosphatase	hydrolysis	hydrolysis of a 3'-PO4 from either single-stranded polynucleotides or free 3'-mononucleotides	Tequatrovirus T4	-	
3.1.4.41	sphingomyelin phosphodiesterase D	hydrolysis	-	Loxosceles intermedia	P0CE81	
3.2.1.1	alpha-amylase	hydrolysis	-	Acarus siro	-	
3.2.1.1	alpha-amylase	hydrolysis	-	Aleuroglyphus ovatus	-	
3.2.1.1	alpha-amylase	hydrolysis	-	Caloglyphus redickorzevi	-	
3.2.1.1	alpha-amylase	hydrolysis	-	Carpoglyphus lactis	-	
3.2.1.1	alpha-amylase	hydrolysis	-	Chortoglyphus arcuatus	-	
3.2.1.1	alpha-amylase	hydrolysis	-	Dermatophagoides farinae	-	
3.2.1.1	alpha-amylase	hydrolysis	-	Lepidoglyphus destructor	-	
3.2.1.1	alpha-amylase	hydrolysis	-	Tyroborus lini	-	
3.2.1.1	alpha-amylase	hydrolysis	-	Tyrophagus putrescentiae	-	
3.2.1.10	oligo-1,6-glucosidase	hydrolysis	-	Mus musculus	-	
3.2.1.108	lactase	hydrolysis	-	Homo sapiens	-	
3.2.1.108	lactase	hydrolysis	-	Rattus norvegicus	-	
3.2.1.114	mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase	hydrolysis	-	Canavalia ensiformis	-	
3.2.1.114	mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase	hydrolysis	-	Drosophila melanogaster	Q24451	
3.2.1.114	mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase	hydrolysis	-	Homo sapiens	-	
3.2.1.115	branched-dextran exo-1,2-alpha-glucosidase	hydrolysis	-	Flavobacterium sp.	-	
3.2.1.123	endoglycosylceramidase	hydrolysis	EGALC hydrolyzes 6-gala series glycosphingolipids to produce intact oligosaccharides and ceramides	Rhodococcus equi	-	
3.2.1.127	1,6-alpha-L-fucosidase	hydrolysis	lysosomal hydrolase, identified in tissues and serum, serum FUCA activity correlated with early detection in hepatocellular carcinoma	Homo sapiens	-	
3.2.1.129	endo-alpha-sialidase	hydrolysis	-	Escherichia phage K1F	-	
3.2.1.133	glucan 1,4-alpha-maltohydrolase	hydrolysis	-	Bacillus sp. (in: Bacteria)	-	
3.2.1.133	glucan 1,4-alpha-maltohydrolase	hydrolysis	-	Geobacillus stearothermophilus	-	
3.2.1.133	glucan 1,4-alpha-maltohydrolase	hydrolysis	-	Thermus sp.	-	
3.2.1.135	neopullulanase	hydrolysis	-	Bifidobacterium breve	-	
3.2.1.135	neopullulanase	hydrolysis	-	Lactiplantibacillus plantarum	-	
3.2.1.135	neopullulanase	hydrolysis	-	Thermoanaerobacter ethanolicus	-	
3.2.1.135	neopullulanase	hydrolysis	only enzymatic activity on alpha-1,6-glusocidic linkages of pullulan, not on alpha-1,4-glucosidic bonds	uncultured microorganism	A7LI67	
3.2.1.136	glucuronoarabinoxylan endo-1,4-beta-xylanase	hydrolysis	-	Thermomyces lanuginosus	-	
3.2.1.136	glucuronoarabinoxylan endo-1,4-beta-xylanase	hydrolysis	facilitating hemicellulose degradation	Aspergillus niger	-	
3.2.1.136	glucuronoarabinoxylan endo-1,4-beta-xylanase	hydrolysis	facilitating hemicellulose degradation	Trichoderma longibrachiatum	-	
3.2.1.136	glucuronoarabinoxylan endo-1,4-beta-xylanase	hydrolysis	or beta-1,4-glycosidic linkages in major plant structural polysaccharides	Cellulomonas flavigena	A1XM14	
3.2.1.139	alpha-glucuronidase	hydrolysis	-	Geobacillus stearothermophilus	Q09LY5	
3.2.1.139	alpha-glucuronidase	hydrolysis	-	Scheffersomyces stipitis	A3LY17	
3.2.1.140	lacto-N-biosidase	hydrolysis	-	Bifidobacterium bifidum	B3TLD6	
3.2.1.140	lacto-N-biosidase	hydrolysis	-	Bifidobacterium longum	-	
3.2.1.141	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase	hydrolysis	-	Metallosphaera hakonensis	-	
3.2.1.141	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase	hydrolysis	-	Saccharolobus solfataricus	-	
3.2.1.143	poly(ADP-ribose) glycohydrolase	hydrolysis	-	Bos taurus	-	
3.2.1.143	poly(ADP-ribose) glycohydrolase	hydrolysis	-	Homo sapiens	-	
3.2.1.143	poly(ADP-ribose) glycohydrolase	hydrolysis	-	Mus musculus	-	
3.2.1.146	beta-galactofuranosidase	hydrolysis	-	Penicillium fellutanum	-	
3.2.1.146	beta-galactofuranosidase	hydrolysis	-	Talaromyces varians	-	
3.2.1.151	xyloglucan-specific endo-beta-1,4-glucanase	hydrolysis	-	Gossypium arboreum	B7SCZ6	
3.2.1.151	xyloglucan-specific endo-beta-1,4-glucanase	hydrolysis	-	Gossypium barbadense	-	
3.2.1.151	xyloglucan-specific endo-beta-1,4-glucanase	hydrolysis	-	Gossypium hirsutum	B2KL33, B2KL34, Q7Y252	
3.2.1.151	xyloglucan-specific endo-beta-1,4-glucanase	hydrolysis	-	Gossypium raimondii	B7SCZ7	
3.2.1.151	xyloglucan-specific endo-beta-1,4-glucanase	hydrolysis	-	Solanum lycopersicum	Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SDX0, Q9SLN9	
3.2.1.152	mannosylglycoprotein endo-beta-mannosidase	hydrolysis	-	Arabidopsis thaliana	Q75W54	
3.2.1.152	mannosylglycoprotein endo-beta-mannosidase	hydrolysis	-	Lilium longiflorum	Q5H7P5	
3.2.1.153	fructan beta-(2,1)-fructosidase	hydrolysis	-	Cichorium intybus	-	
3.2.1.153	fructan beta-(2,1)-fructosidase	hydrolysis	-	Vernonia herbacea	-	
3.2.1.153	fructan beta-(2,1)-fructosidase	hydrolysis	hydrolytic cleavage of terminal fructosyl residues off inulin	Cichorium intybus	-	
3.2.1.153	fructan beta-(2,1)-fructosidase	hydrolysis	hydrolytic cleavage of terminal fructosyl residues off inulin	Vernonia herbacea	A9JIF3	
3.2.1.154	fructan beta-(2,6)-fructosidase	hydrolysis	-	Beta vulgaris	Q70XE6	
3.2.1.161	beta-apiosyl-beta-glucosidase	hydrolysis	beta-glycosidic bond between glycogen and aglycone of disaccharide glycosides, no hydrolysis of bond of two saccharides, barely active on monosaccharide glycosides	Viburnum furcatum	-	
3.2.1.165	exo-1,4-beta-D-glucosaminidase	hydrolysis	-	Amycolatopsis orientalis	Q56F26	
3.2.1.165	exo-1,4-beta-D-glucosaminidase	hydrolysis	cleaving 2-amino-2-deoxy-D-glucopyranose off oligosaccharides	Amycolatopsis orientalis	-	
3.2.1.17	lysozyme	hydrolysis	catalyzes the hydrolysis of bacterial cell walls	Apostichopus japonicus	-	
3.2.1.17	lysozyme	hydrolysis	cleaving the beta-1,4 glycosidic bond between N-acetyl-D-glucosamine (NAG) and N-acetylmuramic acid (NAM) in the peptidoglycan layer	Gadus morhua	-	
3.2.1.17	lysozyme	hydrolysis	hydrolyze the beta-1,4-glycosidic linkage between N-acetylmuramic acid and N-acetylglucosamine of peptidoglycan	Anopheles gambiae	Q17005	
3.2.1.17	lysozyme	hydrolysis	lysozyme has a potent antimicrobial effect due to the hydrolysis of the beta-linkage between muramic acid and N-acetyl glucosamine present in the microbial walls	Gallus gallus	-	
3.2.1.17	lysozyme	hydrolysis	lysozyme hydrolyses 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan	Equus caballus	-	
3.2.1.17	lysozyme	hydrolysis	lysozyme kills bacteria by hydrolyzing beta-1,4-glycosidic linkages between N-acetylglucosamine and N-acetylmuramic acid of the peptidoglycan layer in the bacterial cell wall	Penaeus merguiensis	-	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Acarus siro	-	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Aleuroglyphus ovatus	-	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Apis mellifera	-	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Aspergillus niger	-	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Aspergillus niveus	-	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Beta vulgaris	-	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Caloglyphus redickorzevi	-	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Carpoglyphus lactis	-	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Chilo suppressalis	-	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Chortoglyphus arcuatus	-	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Dermatophagoides farinae	-	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Geobacillus stearothermophilus	-	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Lepidoglyphus destructor	-	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Oryza sativa	-	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Quesada gigas	-	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Saccharolobus solfataricus	P0CD66	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Saccharomyces cerevisiae	-	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Tyroborus lini	-	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Tyrophagus putrescentiae	-	
3.2.1.20	alpha-glucosidase	hydrolysis	-	Zea mays	-	
3.2.1.207	mannosyl-oligosaccharide alpha-1,3-glucosidase	hydrolysis	remove second glucose residue of glycans, incubation time 10 min at 37°C	Rattus norvegicus	-	
3.2.1.21	beta-glucosidase	hydrolysis	-	Chilo suppressalis	-	
3.2.1.23	beta-galactosidase	hydrolysis	-	Geobacillus stearothermophilus	-	
3.2.1.26	beta-fructofuranosidase	hydrolysis	-	Allium cepa	O81083	
3.2.1.26	beta-fructofuranosidase	hydrolysis	-	Bambusa oldhamii	-	
3.2.1.26	beta-fructofuranosidase	hydrolysis	-	Cichorium intybus	-	
3.2.1.26	beta-fructofuranosidase	hydrolysis	-	Cyberlindnera jadinii	-	
3.2.1.26	beta-fructofuranosidase	hydrolysis	-	Helianthus tuberosus	-	
3.2.1.26	beta-fructofuranosidase	hydrolysis	-	Oryza sativa	A2X5P7, Q01IS7, Q01IS8	
3.2.1.26	beta-fructofuranosidase	hydrolysis	-	Phaffia rhodozyma	B8YJM2	
3.2.1.26	beta-fructofuranosidase	hydrolysis	-	Populus alba	-	
3.2.1.26	beta-fructofuranosidase	hydrolysis	-	Pteris deflexa	-	
3.2.1.26	beta-fructofuranosidase	hydrolysis	-	Saccharum officinarum	-	
3.2.1.26	beta-fructofuranosidase	hydrolysis	-	Solanum lycopersicum	P29000	
3.2.1.26	beta-fructofuranosidase	hydrolysis	-	Vitis vinifera	-	
3.2.1.26	beta-fructofuranosidase	hydrolysis	-	Zymomonas mobilis	-	
3.2.1.3	glucan 1,4-alpha-glucosidase	hydrolysis	-	Aspergillus niveus	-	
3.2.1.3	glucan 1,4-alpha-glucosidase	hydrolysis	-	Caldanaerobacter subterraneus subsp. tengcongensis MB4	-	
3.2.1.3	glucan 1,4-alpha-glucosidase	hydrolysis	-	Pyricularia oryzae	-	
3.2.1.4	cellulase	hydrolysis	-	Pseudomonas putida	-	
3.2.1.51	alpha-L-fucosidase	hydrolysis	-	Homo sapiens	P04066	
3.2.1.51	alpha-L-fucosidase	hydrolysis	of glycosidic bond	Homo sapiens	-	
3.2.1.8	endo-1,4-beta-xylanase	hydrolysis	-	Aspergillus niger	-	
3.2.1.8	endo-1,4-beta-xylanase	hydrolysis	-	Cellulomonas flavigena	A1XM14, A1XM14	
3.2.1.8	endo-1,4-beta-xylanase	hydrolysis	-	Thermomyces lanuginosus	-	
3.2.1.8	endo-1,4-beta-xylanase	hydrolysis	-	Trichoderma longibrachiatum	-	
3.2.1.80	fructan beta-fructosidase	hydrolysis	of inulin	Papiliotrema aurea	-	
3.2.1.84	glucan 1,3-alpha-glucosidase	hydrolysis	of alpha-1,3-glucan, C-terminal domain with catalytic activity and low mutan-binding activity, N-terminal domain with mutan-binding activity	Paenibacillus sp.	E16590	
3.2.2.22	rRNA N-glycosylase	hydrolysis	-	Abrus precatorius	-	
3.2.2.22	rRNA N-glycosylase	hydrolysis	-	Escherichia coli	Q8KTU8	
3.2.2.22	rRNA N-glycosylase	hydrolysis	-	Hypsizygus marmoreus	-	
3.2.2.22	rRNA N-glycosylase	hydrolysis	-	Momordica charantia	-	
3.2.2.22	rRNA N-glycosylase	hydrolysis	-	Phytolacca americana	-	
3.2.2.22	rRNA N-glycosylase	hydrolysis	-	Phytolacca dioica	P84853, P84854	
3.2.2.22	rRNA N-glycosylase	hydrolysis	-	Ricinus communis	P02879	
3.2.2.22	rRNA N-glycosylase	hydrolysis	-	Saponaria officinalis	-	
3.2.2.22	rRNA N-glycosylase	hydrolysis	-	Silene chalcedonica	-	
3.2.2.22	rRNA N-glycosylase	hydrolysis	-	Suregada multiflora	-	
3.2.2.22	rRNA N-glycosylase	hydrolysis	-	Trichosanthes kirilowii	P24478	
3.2.2.22	rRNA N-glycosylase	hydrolysis	removal of adenine residue from rRNA loop	Ricinus communis	-	
3.2.2.22	rRNA N-glycosylase	hydrolysis	removal of specific adenine of ribosomal RNA	Ricinus communis	-	
3.2.2.22	rRNA N-glycosylase	hydrolysis	removal of specific adenine residue from eukaryotic larger rRNA subunit, inhibiting translation, N-glycosidase independent induction of apoptosis	Saponaria officinalis	-	
3.2.2.22	rRNA N-glycosylase	hydrolysis	rRNA N-glycosidase activity	Bougainvillea x buttiana	Q4U473	
3.4.11.10	bacterial leucyl aminopeptidase	hydrolysis	-	Escherichia coli	-	
3.4.11.2	membrane alanyl aminopeptidase	hydrolysis	-	Escherichia coli	-	
3.4.11.24	aminopeptidase S	hydrolysis	-	Streptomyces thermocyaneoviolaceus	-	
3.4.11.4	tripeptide aminopeptidase	hydrolysis	-	Escherichia coli	-	
3.4.11.7	glutamyl aminopeptidase	hydrolysis	-	Latilactobacillus curvatus	-	
3.4.16.4	serine-type D-Ala-D-Ala carboxypeptidase	hydrolysis	-	Actinomadura sp. R39	P39045	
3.4.16.4	serine-type D-Ala-D-Ala carboxypeptidase	hydrolysis	-	Escherichia coli K-12	P0AEB2	
3.4.17.1	carboxypeptidase A	hydrolysis	-	Aspergillus niger	-	
3.4.17.1	carboxypeptidase A	hydrolysis	-	Phaffia rhodozyma	-	
3.4.17.13	Muramoyltetrapeptide carboxypeptidase	hydrolysis	-	Acyrthosiphon pisum	B9ZYY6	
3.4.21.22	coagulation factor IXa	hydrolysis	-	Homo sapiens	-	
3.4.21.26	prolyl oligopeptidase	hydrolysis	-	Bos taurus	-	
3.4.21.26	prolyl oligopeptidase	hydrolysis	-	Canis lupus familiaris	-	
3.4.21.26	prolyl oligopeptidase	hydrolysis	-	Elizabethkingia meningoseptica	-	
3.4.21.26	prolyl oligopeptidase	hydrolysis	-	Homo sapiens	-	
3.4.21.26	prolyl oligopeptidase	hydrolysis	-	Mus musculus	-	
3.4.21.26	prolyl oligopeptidase	hydrolysis	-	Oryctolagus cuniculus	-	
3.4.21.26	prolyl oligopeptidase	hydrolysis	-	Rattus norvegicus	-	
3.4.21.26	prolyl oligopeptidase	hydrolysis	-	Sus scrofa	P23687	
3.4.21.26	prolyl oligopeptidase	hydrolysis	-	Trypanosoma brucei brucei	Q6HA27	
3.4.22.15	cathepsin L	hydrolysis	-	Homo sapiens	-	
3.4.22.2	papain	hydrolysis	-	Carica papaya	-	
3.4.22.30	Caricain	hydrolysis	-	Carica papaya	P10056	
3.4.22.51	cruzipain	hydrolysis	-	Trypanosoma cruzi	-	
3.4.22.6	chymopapain	hydrolysis	-	Carica papaya	P14080	
3.4.22.B5	CPB protease	hydrolysis	-	Phytomonas serpens	-	
3.4.24.83	anthrax lethal factor endopeptidase	hydrolysis	-	Bacillus anthracis	-	
3.4.24.B12	ADAMTS5 endopeptidase	hydrolysis	endoproteolytic cleavage of specific Glu-Xaa bonds within the core protein of aggrecan	Homo sapiens	Q9UNA0	
3.4.24.B24	L-alanine-D-glutamate endopeptidase	hydrolysis	-	Bacillus subtilis	O34360	
3.4.24.B24	L-alanine-D-glutamate endopeptidase	hydrolysis	-	Listeria phage A500	-	
3.5.1.10	formyltetrahydrofolate deformylase	hydrolysis	-	Rattus norvegicus	-	
3.5.1.102	2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5'-monophosphate deformylase	hydrolysis	-	Methanocaldococcus jannaschii	Q57580	
3.5.1.13	aryl-acylamidase	hydrolysis	rate determining step of hydrolysis of this substrate is acylation	Homo sapiens	-	
3.5.1.14	N-acyl-aliphatic-L-amino acid amidohydrolase	hydrolysis	-	Aspergillus oryzae	-	
3.5.1.23	ceramidase	hydrolysis	-	Cricetulus griseus	-	
3.5.1.23	ceramidase	hydrolysis	-	Drosophila melanogaster	Q9VA70	
3.5.1.23	ceramidase	hydrolysis	-	Homo sapiens	Q5QJU3, Q8TDN7, Q9NR71, Q9NUN7	
3.5.1.23	ceramidase	hydrolysis	-	Mus musculus	Q8R4X1, Q8VD53, Q9D099, Q9JHE3, Q9WV54	
3.5.1.23	ceramidase	hydrolysis	-	Oryza sativa	-	
3.5.1.23	ceramidase	hydrolysis	-	Pseudomonas aeruginosa	Q9I596	
3.5.1.23	ceramidase	hydrolysis	-	Rattus norvegicus	Q91XT9, Q91XT9	
3.5.1.24	choloylglycine hydrolase	hydrolysis	-	Bifidobacterium bifidum	Q6R974	
3.5.1.24	choloylglycine hydrolase	hydrolysis	-	Bifidobacterium longum	Q9KK62	
3.5.1.24	choloylglycine hydrolase	hydrolysis	-	Bifidobacterium longum subsp. infantis	-	
3.5.1.24	choloylglycine hydrolase	hydrolysis	-	Brevibacillus sp.	-	
3.5.1.24	choloylglycine hydrolase	hydrolysis	-	Clostridium perfringens	P54965, P54965	
3.5.1.24	choloylglycine hydrolase	hydrolysis	-	Enterococcus faecium	Q83YZ2	
3.5.1.24	choloylglycine hydrolase	hydrolysis	-	Lactiplantibacillus plantarum	Q06115	
3.5.1.24	choloylglycine hydrolase	hydrolysis	-	Lactiplantibacillus plantarum WCFS1	Q06115, B9V401	
3.5.1.24	choloylglycine hydrolase	hydrolysis	-	Lactobacillus johnsonii	Q9F660 and P97038	
3.5.1.24	choloylglycine hydrolase	hydrolysis	-	Limosilactobacillus reuteri	-	
3.5.1.24	choloylglycine hydrolase	hydrolysis	-	Listeria monocytogenes	Q8Y5J3	
3.5.1.24	choloylglycine hydrolase	hydrolysis	-	Methanobrevibacter smithii	-	
3.5.1.44	protein-glutamine glutaminase	hydrolysis	-	Chryseobacterium proteolyticum	-	
3.5.1.52	peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase	hydrolysis	-	Homo sapiens	-	
3.5.1.52	peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase	hydrolysis	-	Rattus norvegicus	Q5XI55	
3.5.1.52	peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase	hydrolysis	-	Saccharomyces cerevisiae	Q02890	
3.5.1.77	N-carbamoyl-D-amino-acid hydrolase	hydrolysis	-	Agrobacterium tumefaciens	-	
3.5.1.77	N-carbamoyl-D-amino-acid hydrolase	hydrolysis	-	Ralstonia pickettii	-	
3.5.1.93	glutaryl-7-aminocephalosporanic-acid acylase	hydrolysis	-	Achromobacter xylosoxidans	-	
3.5.1.93	glutaryl-7-aminocephalosporanic-acid acylase	hydrolysis	-	Pseudomonas sp.	-	
3.5.1.98	histone deacetylase	hydrolysis	-	Homo sapiens	Q9BY41	
3.5.1.98	histone deacetylase	hydrolysis	-	Mus musculus	Q9Z2V5	
3.5.1.98	histone deacetylase	hydrolysis	-	Rattus norvegicus	-	
3.5.2.12	6-aminohexanoate-cyclic-dimer hydrolase	hydrolysis	-	Acidovorax sp. T31	B7XBZ8, B7XBZ8	
3.5.2.12	6-aminohexanoate-cyclic-dimer hydrolase	hydrolysis	-	Cupriavidus sp. T7	B7XBZ7, B7XBZ7	
3.5.2.12	6-aminohexanoate-cyclic-dimer hydrolase	hydrolysis	-	Cupriavidus sp. U124	B7XBZ9, B7XBZ9	
3.5.2.12	6-aminohexanoate-cyclic-dimer hydrolase	hydrolysis	-	Rhodococcus sp. U-224	B5MF68	
3.5.2.12	6-aminohexanoate-cyclic-dimer hydrolase	hydrolysis	-	Sphingomonas sp. U238	B7XC00, B7XC00	
3.5.2.3	dihydroorotase	hydrolysis	-	Rattus norvegicus	-	
3.5.2.5	allantoinase	hydrolysis	-	Escherichia coli	P77671	
3.5.2.5	allantoinase	hydrolysis	-	Pseudomonas fluorescens	B5L363	
3.5.2.6	beta-lactamase	hydrolysis	-	Acinetobacter sp.	-	
3.5.2.6	beta-lactamase	hydrolysis	-	Enterobacter cloacae	-	
3.5.2.6	beta-lactamase	hydrolysis	-	Klebsiella pneumoniae	-	
3.5.2.6	beta-lactamase	hydrolysis	-	Pseudomonas aeruginosa	-	
3.5.2.6	beta-lactamase	hydrolysis	-	Pseudomonas putida	Q8GCU7	
3.5.2.6	beta-lactamase	hydrolysis	-	Serratia marcescens	-	
3.5.2.9	5-oxoprolinase (ATP-hydrolysing)	hydrolysis	-	Arabidopsis thaliana	Q9FIZ7	
3.5.3.12	agmatine deiminase	hydrolysis	involved in agmatine deiminase system	Streptococcus mutans	-	
3.5.3.18	dimethylargininase	hydrolysis	-	Bos taurus	Q3SX44	
3.5.3.18	dimethylargininase	hydrolysis	-	Homo sapiens	O94760, O95865	
3.5.3.18	dimethylargininase	hydrolysis	-	Mus musculus	Q99LD8, Q9CWS0	
3.5.3.18	dimethylargininase	hydrolysis	-	Oryctolagus cuniculus	-	
3.5.3.18	dimethylargininase	hydrolysis	-	Rattus norvegicus	O08557, Q6MG60	
3.5.4.9	methenyltetrahydrofolate cyclohydrolase	hydrolysis	-	Homo sapiens	-	
3.5.4.9	methenyltetrahydrofolate cyclohydrolase	hydrolysis	-	Leishmania major	-	
3.5.4.9	methenyltetrahydrofolate cyclohydrolase	hydrolysis	-	Saccharomyces cerevisiae	P07245, P09440	
3.5.5.5	Arylacetonitrilase	hydrolysis	-	Pseudomonas putida	B0LAW4	
3.5.99.7	1-aminocyclopropane-1-carboxylate deaminase	hydrolysis	-	Pseudomonas fluorescens	-	
3.5.99.7	1-aminocyclopropane-1-carboxylate deaminase	hydrolysis	-	Pseudomonas putida	-	
3.5.99.9	2-nitroimidazole nitrohydrolase	hydrolysis	-	Mycobacterium sp.	F4ZCI3	
3.6.1.29	bis(5'-adenosyl)-triphosphatase	hydrolysis	-	Homo sapiens	-	
3.6.5.1	heterotrimeric G-protein GTPase	hydrolysis	-	Homo sapiens	-	
3.6.5.1	heterotrimeric G-protein GTPase	hydrolysis	-	Mus musculus	-	
3.6.5.1	heterotrimeric G-protein GTPase	hydrolysis	-	Rattus norvegicus	-	
3.6.5.1	heterotrimeric G-protein GTPase	hydrolysis	-	Saccharomyces pombe	-	
3.6.5.3	protein-synthesizing GTPase	hydrolysis	-	Escherichia coli	P0CE48	
3.6.5.3	protein-synthesizing GTPase	hydrolysis	-	Saccharolobus solfataricus	-	
3.6.5.3	protein-synthesizing GTPase	hydrolysis	-	Saccharomyces cerevisiae	P32501	
3.6.5.3	protein-synthesizing GTPase	hydrolysis	-	Thermus aquaticus	Q01698	
3.6.5.3	protein-synthesizing GTPase	hydrolysis	-	Thermus thermophilus	P60338	
3.8.1.5	haloalkane dehalogenase	hydrolysis	-	Alcaligenes sp.	-	
3.8.1.8	atrazine chlorohydrolase	hydrolysis	-	Arthrobacter sp.	-	
3.8.1.8	atrazine chlorohydrolase	hydrolysis	-	Pseudomonas sp.	-	
4.2.99.18	DNA-(apurinic or apyrimidinic site) lyase	hydrolysis	-	Homo sapiens	-	
4.4.1.1	cystathionine gamma-lyase	hydrolysis	-	Mus musculus	-	
4.4.1.1	cystathionine gamma-lyase	hydrolysis	-	Rattus norvegicus	-	
4.6.1.24	ribonuclease T1	hydrolysis	-	Aspergillus oryzae	-	
5.6.1.7	chaperonin ATPase	hydrolysis	-	Escherichia coli	P0A6F5, P0A6F5	
5.6.1.7	chaperonin ATPase	hydrolysis	-	Thermoplasma acidophilum	-	
5.6.1.7	chaperonin ATPase	hydrolysis	formation of the closed lid requires ATP hydrolysis	Bos taurus	-	
5.6.1.7	chaperonin ATPase	hydrolysis	proteins of size up to 70 kDA fold via cis mechanism during GroEL-ES assisted pathway, proteins of size upper 70 kDa fold via trans mechanism by GroES-binding	Escherichia coli	-	
6.3.2.13	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-2,6-diaminopimelate ligase	hydrolysis	-	Pseudomonas aeruginosa	-	
6.3.4.6	urea carboxylase	hydrolysis	yeast enzyme	Saccharomyces cerevisiae	P32528	
7.4.2.7	ABC-type alpha-factor-pheromone transporter	hydrolysis	-	Saccharomyces cerevisiae	-