1.7.3.3 factor-independent urate hydroxylase additional information the enzyme catalyzes the degradation of urate to [S]-allantoin through 5-hydroxyisourate as a metastable intermediate Aspergillus flavus Q00511 1.8.1.2 assimilatory sulfite reductase (NADPH) additional information sodium salts of thiosulfate and sulfate do not serve as the electron acceptor for reduced F420 oxidation by Fsr. Also, Fsr can not use NADH and NADPH for the reduction of sulfite. Methanocaldococcus jannaschii - 1.8.1.2 assimilatory sulfite reductase (NADPH) additional information the N-terminal half of Fsr represents a H2F420 dehydrogenase and the C-terminal half a dissimilatory-type siroheme sulfite reductase, and Fsr catalyzes the corresponding partial reactions Methanocaldococcus jannaschii - 1.8.1.9 thioredoxin-disulfide reductase additional information the enzyme utilizes oxygen, requires NADH or NADPH, and readily generates the reduced paraquat radical Mus musculus - 1.8.3.1 sulfite oxidase additional information the enzyme also functions as a selenite oxidase Arabidopsis thaliana - 1.8.7.1 assimilatory sulfite reductase (ferredoxin) additional information sodium salts of thiosulfate and sulfate does not serve as the electron acceptor for reduced F420 oxidation by Fsr. Also, Fsr can not use NADH and NADPH for the reduction of sulfite. Methanocaldococcus jannaschii - 1.8.7.1 assimilatory sulfite reductase (ferredoxin) additional information the N-terminal half of Fsr represents a H2F420 dehydrogenase and the C-terminal half a dissimilatory-type siroheme sulfite reductase, and Fsr catalyzes the corresponding partial reactions Methanocaldococcus jannaschii - 3.1.3.18 phosphoglycolate phosphatase additional information - Nicotiana tabacum - 3.1.3.43 [pyruvate dehydrogenase (acetyl-transferring)]-phosphatase additional information PDP activity and protein content is higher in fast-twitch oxidative glycolytic muscles, food deprivation decreases PDP activity in all muscle types, PDP2 declines in fast-twitch oxidative glycolytic muscle Rattus norvegicus - 3.1.3.43 [pyruvate dehydrogenase (acetyl-transferring)]-phosphatase additional information PDP1 belongs to the PPM family of protein serine/threonine phosphatases Rattus norvegicus O88483 3.1.3.45 3-deoxy-manno-octulosonate-8-phosphatase additional information KDO-phosphatase is not essential for viability of Escherichia coli Escherichia coli - 3.1.3.53 [myosin-light-chain] phosphatase additional information cardiac derived MYPT2 and smooth muscle derived MYPT2 have similar properties Mammalia - 3.1.3.53 [myosin-light-chain] phosphatase additional information MYTP1 binds the catalytic subunit of type 1 phosphatase delta, binds many proteins like myosin II, ezrin, radixin, moesin, a-adducin, tau, MAP, elongation factor-1a, ZIP kinase, RhoA-GTP, key reaction is dephosphorylation of myosin II but also in cell migration, cell division Mammalia - 3.1.3.60 phosphoenolpyruvate phosphatase additional information PEP phosphatase increases as active Fe decreases Vitis labrusca x Vitis vinifera - 3.1.4.12 sphingomyelin phosphodiesterase additional information SMase belongs to the family of interfacial enzymes that carry out processive catalytic turnover at the interface, SMase binds rapidly and avidly to sphingomyelin vesicles and it is fully active as a monomer at the interface Bacillus cereus - 3.4.21.22 coagulation factor IXa additional information amidolytic activity Homo sapiens - 3.4.22.61 caspase-8 additional information caspase-8 also regulates cell motility Mus musculus O89110 3.4.24.81 ADAM10 endopeptidase additional information ADAM10 mediates the epidermal growth factor-induced CD44 cleavage by the small monomeric GTPase Rac1 Homo sapiens - 3.6.5.3 protein-synthesizing GTPase additional information anti-association activity for splitted 70S ribosomes subunits Escherichia coli - 3.6.5.3 protein-synthesizing GTPase additional information influence on translation initiation pathway and ribosomal subunit joining Saccharomyces cerevisiae - 3.6.5.3 protein-synthesizing GTPase additional information together with ribosome recycling factor and GTP transient split of 70S ribosomes into subunits Escherichia coli - 4.1.3.43 4-hydroxy-2-oxohexanoate aldolase additional information BphI exhibits a compulsory order mechanism, with pyruvate binding first Paraburkholderia xenovorans - 4.1.99.26 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethylpyrrolidin-2-one synthase additional information enzyme MftC catalyzes two distinct chemistries in the same active, an oxidative decarboxylation of the C-terminus and a subsequent redox neutral C-C bond formation Mycobacterium ulcerans A0PM49, A0PM49 4.2.1.84 nitrile hydratase additional information a new biocatalytic mechanism is proposed, that is based on crystallographic data of the active center Rhodococcus erythropolis - 4.2.2.5 chondroitin AC lyase additional information no beta-elimination Pedobacter heparinus - 4.2.2.7 heparin lyase additional information random endolytic attack Pedobacter heparinus - 4.2.2.7 heparin lyase additional information random endolytic attack Rattus norvegicus - 4.2.3.22 germacradienol synthase additional information germacradienol/geosmin synthase is a bifunctional enzyme in which the N-terminal domain of the protein converts farnesyl diphosphate, while the C-terminal domain catalyzes the transformation of germacradienol to geosmin Streptomyces coelicolor - 4.2.3.22 germacradienol synthase additional information putative function is as a germacradienol synthase/terpene cyclase Streptomyces peucetius B0FLN6 4.2.3.4 3-dehydroquinate synthase additional information DHQS itself is of interest because it apparently catalyzes five individual reactions, alcohol oxidation, phosphate omega-elimination, carbonyl reduction, ring opening and intramolecular aldol condensation, in a single active site as well as being a drug target Xanthomonas oryzae - 4.2.99.18 DNA-(apurinic or apyrimidinic site) lyase additional information APE1 possesses endonuclease, exonuclease and phosphodiesterase activity Homo sapiens - 4.4.1.13 cysteine-S-conjugate beta-lyase additional information - Fusobacterium varium - 4.4.1.13 cysteine-S-conjugate beta-lyase additional information - Homo sapiens - 4.4.1.13 cysteine-S-conjugate beta-lyase additional information - Rattus norvegicus - 4.4.1.13 cysteine-S-conjugate beta-lyase additional information identical with glutamine transaminase K Rattus norvegicus - 4.4.1.13 cysteine-S-conjugate beta-lyase additional information kynureninase reaction and beta-elimination Rattus norvegicus - 4.4.1.13 cysteine-S-conjugate beta-lyase additional information shows identity with a soluble kynurenine aminotransferase from rat brain Rattus norvegicus - 4.4.1.14 1-aminocyclopropane-1-carboxylate synthase additional information 14-3-3 protein may inhibit binding to ethylene overproducer 1 proteins, resulting in 1-aminocyclopropane-1-carboxylate and ethylene synthesis Oryza sativa Q10DK7 4.4.1.14 1-aminocyclopropane-1-carboxylate synthase additional information by Agrobacterium mediated transformation, two transgenic pineapple lines have been produced containing co-suppression constructs designed to down-regulate the expression of the ACACS2 gene Ananas comosus - 4.4.1.14 1-aminocyclopropane-1-carboxylate synthase additional information high levels of expression of CyACS1 in the necrotic inflorescences of wild-type Cymbidium at high temperatures, no bud necrosis in the mericlone mutant, but application of exogenous ACC or ethephon to the young inflorescences of nhn restored the high-temperature necrosis response Cymbidium hybrid cultivar A0JBY6 4.4.1.14 1-aminocyclopropane-1-carboxylate synthase additional information LE-ACS3 shows a strong interaction with the protein ethylene overproducer 1, the C-terminal tail of ACS is essential for the interaction with ethylene overproducer 1 and signals the proteasome-dependent protein destabilization Solanum lycopersicum Q42881 4.4.1.14 1-aminocyclopropane-1-carboxylate synthase additional information two introns are in the genomic DNA sequence, Southern blot analysis suggests that there might be a multi-gene family encoding for ACC synthase, alignment analysis shows a close association with the wound-inducible ACS of citrus Gossypium hirsutum A9NIT9 4.4.1.20 leukotriene-C4 synthase additional information multiple constructs encoding fusion proteins of green fluorescent protein as the N-terminal part and various truncated variants of human LTC4S as C-terminal part were prepared and transfected into HEK 293/T or COS-7 cells Homo sapiens Q16873 4.4.1.21 S-ribosylhomocysteine lyase additional information absorption and electron paramagnetic resonance spectroscopic studies reveals that the active form of LuxS contains a metal-bound water and a thiolate ion at Cys-83, an invariant Arg-39 in the active site is partially responsible for stabilizing the thiolate anion of Cys-83 Escherichia coli P45578 4.4.1.21 S-ribosylhomocysteine lyase additional information Bacillus subtilis can use methionine as sole sulfur source, the BsluxS knockout mutant grows poorly in the presence of methionine compared to the wild-type strain, methionine utilization requires first its conversion to homocysteine Bacillus subtilis O34667 4.8.1.2 aliphatic aldoxime dehydratase additional information electronic absorption, resonance Raman spectroscopy, electronic paramagnetic resonance and rapid scanning spectroscopy shows that ferric OxdB contains a six-coordinate high-spin heme, the substrate is bound to the ferric heme via its oxygen atom, the coordination structure of the heme-aldoxime complex changes redox-dependent Bacillus sp. (in: Bacteria) - 4.8.1.2 aliphatic aldoxime dehydratase additional information electronic absorption, resonance Raman spectroscopy, electronic paramagnetic resonance and rapid scanning spectroscopy shows that ferric OxdB contains a six-coordinate high-spin heme, the substrate is bound to the ferric heme via its oxygen atom, the coordination structure of the heme-aldoxime complex changes redox-dependent Rhodococcus sp. - 4.8.1.2 aliphatic aldoxime dehydratase additional information resonance Raman spectroscopy shows that an reaction intermediate of the hemecontaining enzyme with a highly oxidized heme is formed concomitantly upon direct binding of a substrate Pseudomonas chlororaphis - 5.1.3.2 UDP-glucose 4-epimerase additional information catalyses the interconversion of UDP-Gal and UDPGlc Escherichia coli - 5.1.3.2 UDP-glucose 4-epimerase additional information catalyses the interconversion of UDP-Gal and UDPGlc Hordeum vulgare Q58IJ6 5.1.3.2 UDP-glucose 4-epimerase additional information HvUGE also catalyses the interconversion of UDP-GalNAc and UDP-GlcNAc, although it is not known if this has any biological significance. Hordeum vulgare Q58IJ6 6.3.2.1 pantoate-beta-alanine ligase (AMP-forming) additional information pantothenate synthetase catalyzes the formation of a pantoyl-adenylate intermediate upon the ordered addition of ATP and pantoate Mycobacterium tuberculosis - 6.3.4.15 biotin-[biotin carboxyl-carrier protein] ligase additional information biotinylation, mediates attachment of biotin to a fusion protein of a biotin acceptor peptide and GLuc, EC 1.13.12.5 Escherichia coli K-12 P06709