3.2.1.68 isoamylase trehalose biosynthesis V 3.2.1.141 4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase trehalose biosynthesis V 5.4.99.15 (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase trehalose biosynthesis V