Information on EC 4.5.1.2 - 3-chloro-D-alanine dehydrochlorinase:
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EC NUMBERCOMMENTARY
4.5.1.2-

RECOMMENDED NAMEGeneOntology No.
3-chloro-D-alanine dehydrochlorinaseGO:0019149

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
3-chloro-D-alanine + H2O = pyruvate + chloride + NH3
show the reaction diagram		----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
beta-replacementPseudomonas putida--34746, 34747, 34748, 34749, 34750, 34751, 34752, 34753
elimination--alpha,beta elimination; of HCl, C-Cl bond cleavage-

PATHWAYKEGG LinkMetaCyc Link
No entries in this field

SYSTEMATIC NAMEIUBMB Comments
3-chloro-D-alanine chloride-lyase (deaminating; pyruvate-forming)A pyridoxal-phosphate protein. Also catalyses beta-replacement reactions, e.g. converts 3-chloro-D-alanine and H2S into D-cysteine and HCl.

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
3-chloro-D-alanine chloride-lyase----
beta-chloro-D-alanine dehydrochlorinase----

CAS REGISTRY NUMBERCOMMENTARY
78990-65-5-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Pseudomonas putida-34752--Manually annotated by BRENDA team
Pseudomonas putidaCR1-134745, 34746, 34747, 34748, 34749, 34750--Manually annotated by BRENDA team
Pseudomonas putidaCR1-1, AKU 86734751, 34753--Manually annotated by BRENDA team
Pseudomonas putida CR1-1CR1-134745--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
3-chloro-D-alanine + 2-mercaptoethanolS-(2-hydroxyethyl)-D-cysteine
show the reaction diagram		Pseudomonas putida--34748-34748?
3-chloro-D-alanine + 2-mercaptoethanolS-(2-hydroxyethyl)-D-cysteine
show the reaction diagram		Pseudomonas putida--34753-34753?
3-chloro-D-alanine + allyl mercaptaneS-allyl-D-cysteine
show the reaction diagram		Pseudomonas putida--34748-34748?
3-chloro-D-alanine + allyl mercaptaneS-allyl-D-cysteine
show the reaction diagram		Pseudomonas putida--34753-34753?
3-chloro-D-alanine + alpha-thiolglycerolS-(2,3-dihydroxypropyl)-D-cysteine
show the reaction diagram		Pseudomonas putida--34748-34748?
3-chloro-D-alanine + alpha-thiolglycerolS-(2,3-dihydroxypropyl)-D-cysteine
show the reaction diagram		Pseudomonas putida--34753-34753?
3-chloro-D-alanine + benzyl-mercaptaneS-benzyl-D-cysteine
show the reaction diagram		Pseudomonas putida--34748-34748?
3-chloro-D-alanine + ethyl thioglycolateS-(ethoxy-carbonyl-methyl)-D-cysteine
show the reaction diagram		Pseudomonas putida--34746-34746ir
3-chloro-D-alanine + ethyl-mercaptaneS-ethyl-D-cysteine
show the reaction diagram		Pseudomonas putida--34748-34748?
3-chloro-D-alanine + ethyl-mercaptaneS-ethyl-D-cysteine
show the reaction diagram		Pseudomonas putida--34753-34753?
3-chloro-D-alanine + H2Opyruvate + HCl + NH3
show the reaction diagram		Pseudomonas putida--34745-34745ir
3-chloro-D-alanine + H2Opyruvate + HCl + NH3
show the reaction diagram		Pseudomonas putida--34746-34746ir
3-chloro-D-alanine + H2Opyruvate + HCl + NH3
show the reaction diagram		Pseudomonas putida--34747-34747ir
3-chloro-D-alanine + H2Opyruvate + HCl + NH3
show the reaction diagram		Pseudomonas putida--34748-34748ir
3-chloro-D-alanine + H2Opyruvate + HCl + NH3
show the reaction diagram		Pseudomonas putida--34750-34750ir
3-chloro-D-alanine + H2Opyruvate + HCl + NH3
show the reaction diagram		Pseudomonas putida--34752-34752ir
3-chloro-D-alanine + H2Opyruvate + HCl + NH3
show the reaction diagram		Pseudomonas putida--34753-34753ir
3-chloro-D-alanine + H2O?
show the reaction diagram		Pseudomonas putida--34745, 34747, 34753---
3-chloro-D-alanine + H2SD-cysteine + HCl
show the reaction diagram		Pseudomonas putida--34745-34745ir
3-chloro-D-alanine + methyl-mercaptaneS-methyl-D-cysteine
show the reaction diagram		Pseudomonas putida--34748-34748?
3-chloro-D-alanine + methyl-mercaptaneS-methyl-D-cysteine
show the reaction diagram		Pseudomonas putida--34753-34753?
3-chloro-D-alanine + n-butyl-mercaptaneS-n-butyl-D-cysteine
show the reaction diagram		Pseudomonas putida--34748-34748?
3-chloro-D-alanine + n-butyl-mercaptaneS-n-butyl-D-cysteine
show the reaction diagram		Pseudomonas putida--34753-34753?
3-chloro-D-alanine + n-propyl-mercaptaneS-n-propyl-D-cysteine
show the reaction diagram		Pseudomonas putida--34748-34748?
3-chloro-D-alanine + n-propyl-mercaptaneS-n-propyl-D-cysteine
show the reaction diagram		Pseudomonas putida--34753-34753?
3-chloro-D-alanine + NaHSD-cysteine + NaCl
show the reaction diagram		Pseudomonas putida--34746-34746ir
3-chloro-D-alanine + NaHSD-cysteine + NaCl
show the reaction diagram		Pseudomonas putida--34747-34747ir
3-chloro-D-alanine + NaHSD-cysteine + NaCl
show the reaction diagram		Pseudomonas putida--34748-34748ir
3-chloro-D-alanine + NaHSD-cysteine + NaCl
show the reaction diagram		Pseudomonas putida--34749-34749ir
3-chloro-D-alanine + NaHSD-cysteine + NaCl
show the reaction diagram		Pseudomonas putida--34750-34750ir
3-chloro-D-alanine + NaHSD-cysteine + NaCl
show the reaction diagram		Pseudomonas putida--34753-34753ir
3-chloro-D-alanine + phenyl-mercaptaneS-phenyl-D-cysteine
show the reaction diagram		Pseudomonas putida--34748-34748?
3-chloro-D-alanine + phenyl-mercaptaneS-phenyl-D-cysteine
show the reaction diagram		Pseudomonas putida--34753-34753?
additional information?-Pseudomonas putida-specific for 3-chloro-D-alanine34745-34745-

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
3-chloro-D-alanine + H2O?
show the reaction diagram		Pseudomonas putida--34745, 34747, 34753--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
pyridoxal 5'-phosphatePseudomonas putida--34745, 34753 2D-image
pyridoxal 5'-phosphatePseudomonas putida-2 mol pyridoxal 5'-phosphate per mol of enzyme34751 2D-image

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
No entries in this field

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
3-chloro-L-alaninePseudomonas putida-competitive34749 2D-image
AgNO3Pseudomonas putida--34751, 34753 2D-image
CuSO3Pseudomonas putida--34751, 34753 2D-image
CycloserinePseudomonas putida--34751, 34753 2D-image
cysteamine dihydrochloridePseudomonas putida--34751 2D-image
D-penicillaminePseudomonas putida--34751, 34753 2D-image
HgCl2Pseudomonas putida--34751, 34753 2D-image
hydroxylamine HClPseudomonas putida--34751 2D-image
iodoacetatePseudomonas putida--34751, 34753 2D-image
KCNPseudomonas putida--34751, 34753 2D-image
L-PenicillaminePseudomonas putida--34751 2D-image
phenylhydrazinePseudomonas putida--34751, 34753 2D-image
Semicarbazide hydrochloridePseudomonas putida--34751 2D-image
Thiol reagentsPseudomonas putida--34751, 34753 2D-image

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.061-3-chloro-D-alaninePseudomonas putida--34747, 34751, 34753 2D-image
8.1-3-chloro-D-alaninePseudomonas putida--34745, 34747 2D-image
0.18-D-CysteinePseudomonas putida--34753 2D-image
46-H2SPseudomonas putida--34745 2D-image
45-NaHSPseudomonas putida--34747 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
310-Pseudomonas putida--34753
311-Pseudomonas putida-alpha,beta elimination34747
additional information-Pseudomonas putida--34745, 34751

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
7.58Pseudomonas putida-D-cysteine synthesis34750
8.5-Pseudomonas putida-cysteine synthesis34745
9-Pseudomonas putida--34747, 34751, 34753

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
710Pseudomonas putida--34745

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
30-Pseudomonas putida--34747, 34750
40-Pseudomonas putida--34745

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
No entries in this field

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
cytoplasmPseudomonas putida--573734745, 34752Manually annotated by BRENDA team
solublePseudomonas putida---34745Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
76000-Pseudomonas putida-sedimentation equilibrium34747, 34751, 34753

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
dimerPseudomonas putida-2 * 38000, SDS-PAGE34747, 34751, 34753

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Pseudomonas putida-34747, 34751, 34753

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
68Pseudomonas putida--34751

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
45-Pseudomonas putida-15 min stable34751

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
several months at -20°CPseudomonas putida-34749

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
CR1-1Pseudomonas putida-34751, 34753

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
34745Yamada, H.; Nagasawa, T.; Ohkishi, H.; Kawakami, B.; Tani, Y.Synthesis of D-cysteine from 3-chloro-D-alanine and hydrogen sulfide by 3-chloro-D-alanine hydrogen chloride-lyase (deaminating) of Pseudomonas putidaBiochem. Biophys. Res. Commun.1001104-11101981Pseudomonas putida PubMed
34746Nagasawa, T.; Hosono, H.; Ohkishi, H.; Yamada, H.Synthesis of S-(carboxymethyl)-D-cysteine by 3-chloro-D-alanine chloride-lyase of pseudomonas putida CR 1-1Appl. Biochem. Biotechnol.8481-4891983Pseudomonas putida PubMed
34747Soda, K.Synthesis of D-cysteine by a new pyridoxal enzymeTrends Biochem. Sci.8152-1531983Pseudomonas putida-
34748Nagasawa, T.; Hosano, H.; Ohkishi, H.; Tani, Y.; Yamada, H.Synthesis of D-cysteine-related amino acids by 3-chloro-D-alanine chloride-lyase of Pseudomonas putida CR 1-1Biochem. Biophys. Res. Commun.111809-8161983Pseudomonas putida PubMed
34749Nagasawa, T.; Hosano, H.; Yamano, H.; Ohkishi, H.; Tani, Y.; Yamada, H.Synthesis of C-cysteine from a racemate of 3-chloroalanine by phenylhydrazine-treated cells of Pseudomonas putida CR 1-1Agric. Biol. Chem.47861-8681983Pseudomonas putida-
34750Nagasawa, T.; Yamano, H.; Hosono, H.; Yamada, H.; Ohkishi, H.; Tani, Y.Enzymatic synthesis of D-cysteine by 3-chloro-D-alanine resistant Pseudomonas putida CR 1-1Agric. Biol. Chem.463003-30101982Pseudomonas putida-
34751Nagasawa, T.; Ohkishi, H.; Kavakami, B.; Yamano, H.; Hosono, H.; Tani, Y.; Yamada, H.3-Chloro-D-alanine chloride-lyase (deaminating) of Pseudomonas putida CR 1.1. Purification and characterization of a novel enzyme occurring in 3-chloro-D-alanine-resistant pseudomonadsJ. Biol. Chem.25713749-137561982Pseudomonas putida PubMed
34752Nagasawa, T.; Ishii, T.; Yamada, H.Physiological comparison of D-cysteine desulfhydrase of Escherichia coli with 3-chloro-D-alanine dehydrochlorinase of Pseudomonas putida CR 1-1Arch. Microbiol.149413-4161988Pseudomonas putida PubMed
34753Nagasawa, T.; Ohkishi, H.; Yamada, H.3-Chloro-D-alanine chloride-lyase (deaminating) of Pseudomonas putida CR 1-1Prog. Clin. Biol. Res.144A365-3751984Pseudomonas putida PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 4.5.1.2)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)