Information on EC 3.6.1.42 - guanosine-diphosphatase:

   PRINT
Please wait a moment until all data are loaded. This message will disappear when all data are loaded.
Mark a special word or phrase in this record:  
Select one or more organisms in this record:

The expected taxonomic range for this enzyme is: Opisthokonta


Show additional data Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

Please login to have access to the AMENDA and FRENDA data

EC NUMBERCOMMENTARY
3.6.1.42-

RECOMMENDED NAMEGeneOntology No.
guanosine-diphosphataseGO:0004382

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
GDP + H2O = GMP + phosphate
show the reaction diagram
stoichiometry of reactionBos taurus-210052
GDP + H2O = GMP + phosphate
show the reaction diagram
----
GDP + H2O = GMP + phosphate
show the reaction diagram
also acts on UDP but not on other nucleoside diphosphates and triphosphatesKluyveromyces lactisQ9HEM6488582
GDP + H2O = GMP + phosphate
show the reaction diagram
also acts on UDPSchizosaccharomyces pombe-655628

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
hydrolysis of diphosphate bonds----
hydrolysis of phosphoric esterKluyveromyces lactisQ9HEM6-488582
hydrolysis of phosphoric esterSchizosaccharomyces pombe--655628

PATHWAYKEGG LinkMetaCyc Link
NIL-RXN-14201

SYSTEMATIC NAMEIUBMB Comments
GDP phosphohydrolaseAlso acts on UDP but not on other nucleoside diphosphates and triphosphates.

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
gda1p (GDA1 gene product)----
Gdap1Candida albicansQ8TGH6-655389
Gdp1pSchizosaccharomyces pombe--655628
GDPase----
GDPaseCandida albicansQ8TGH6-655389
GDPaseKluyveromyces lactisQ9HEM6-488582
GDPaseSchizosaccharomyces pombe--655628
guanosine diphosphatase----
guanosine diphosphataseSchizosaccharomyces pombe--655628
KlGdap1Kluyveromyces lactisQ9HEM6-488582
phosphatase, guanosine di-----
UDPase----

CAS REGISTRY NUMBERCOMMENTARY
98037-56-0-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Bos tauruscalf210052--Manually annotated by BRENDA team
Candida albicansgene gda1655389Q8TGH6SwissProtManually annotated by BRENDA team
Homo sapiensnormal and transforming PCPH proteins: PCPH, PCPH proto-oncogene and mt-PCPH, PCPH oncogene having guanosine diphosphatase activity210055--Manually annotated by BRENDA team
Kluyveromyces lactisenzyme KlGdap1488582Q9HEM6SwissProtManually annotated by BRENDA team
Mesocricetus auratusSyrian hamster, normal and transforming PCPH proteins: PCPH, PCPH proto-oncogene and mt-PCPH, PCPH oncogene having guanosine diphosphatase activity210055--Manually annotated by BRENDA team
Mus musculusnormal and transforming PCPH proteins: PCPH, PCPH proto-oncogene and mt-PCPH, PCPH oncogene having guanosine diphosphatase activity210055--Manually annotated by BRENDA team
Saccharomyces cerevisiae-210056, 210057--Manually annotated by BRENDA team
Saccharomyces cerevisiaestrains G2-25, wild-type and null-mutants for GDA-1210053--Manually annotated by BRENDA team
Saccharomyces cerevisiaewild-type and mutants210054--Manually annotated by BRENDA team
Saccharomyces cerevisiaewild-type and null mutant for guanosine diphosphatase210058--Manually annotated by BRENDA team
Schizosaccharomyces pombe-210056--Manually annotated by BRENDA team
Schizosaccharomyces pombeseveral strains, gene gdp1+655628--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + H2OAMP + phosphate
show the reaction diagram
Candida albicansQ8TGH6-655389--?
ADP + H2OAMP + phosphate
show the reaction diagram
Schizosaccharomyces pombe-low activity655628--?
ATP + H2OADP + phosphate
show the reaction diagram
Schizosaccharomyces pombe-low activity655628--?
GDP + H2OGMP + phosphate
show the reaction diagram
Schizosaccharomyces pombe--655628--?
GDP + H2OGMP + phosphate
show the reaction diagram
Candida albicansQ8TGH6preferred substrate, GDP hydrolysis occurs in the Golgi lumen to give GMP, which is required as antiporter for GDP-mannose transport into the Golgi lumen655389--?
GDP + H2OGMP + phosphate
show the reaction diagram
Schizosaccharomyces pombe-the enzyme has a main role in protein glycosylation, enzyme deficiency leads to N-glycosylation defects of invertase655628--?
GDP + H2OGMP + phosphate
show the reaction diagram
Kluyveromyces lactisQ9HEM6the enzyme's GDPase activity plays a role in guanosine nucleotide sugar transport into Golgi vesicles488582--?
GDP + H2OGMP + phosphate
show the reaction diagram
Kluyveromyces lactisQ9HEM6preferred substrate in presence of Ca2+ or Mn2+488582--?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae--210053-210053?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae--210054-210054?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae-best substrate210053-210053?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae-best substrate210056-210056?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae-best substrate210057-210057?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Bos taurus-best substrate210052-210052?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Schizosaccharomyces pombe-best substrate210056-210056?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae-highly specific for GDP210056-210056?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae-highly specific for GDP210058-210058?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Bos taurus-hydrolyzes free GDP, unable to hydrolyze GDP bound to eIF-2, eukaryotic initiation factor 2, and unable to catalyze the exchange of bound GDP with GTP even in the presence of relatively high molar excess of GTP and Met-tRNA210052-210052?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Mus musculus, Homo sapiens, Mesocricetus auratus-PCPH and mt-PCPH proteins have intrinsic GDPase activity, are GDPases210055-210055?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Bos taurus-possible biological function in maintaining the cellular pool of GTP-GDP210052-210052?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae-cleaves GDP that originates from GDP-mannose after mannosylation of glycoproteins in Golgi complex210054-210054?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae-GDP is a reaction product following outer-chain mannosylation of luminal proteins and inhibitor of mannosyltransferases. It is hypothesized that GMP then returns to the cytosol. GDPase presumably serves as an essential function in processing of yeast glycoproteins210056-210056?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae-GDPase plays a role in O- and N-mannosylation of proteins such as chitinase and carboxypeptidase Y and glycosylation of external invertase and of mannosylation of inositolphosphorylceramides210058-210058-
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae-GDP is known to be an inhibitor of mannosylation reactions in the Golgi lumen, removal of GDP by conversion to GMP, the latter not being an inhibitor of these reactions, would allow mannosylation to proceed210056-210056?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae-guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen210058-210058-
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Bos taurus-GTP is an important steric effector in many biological systems, GDP a potent inhibitor: GDPase activity could be involved in preventing potential inhibitory levels of GDP from accumulating210052-210052?
UDP + H2OUMP + phosphate
show the reaction diagram
Mus musculus, Homo sapiens--210055--?
UDP + H2OUMP + phosphate
show the reaction diagram
Schizosaccharomyces pombe--655628--?
UDP + H2OUMP + phosphate
show the reaction diagram
Mesocricetus auratus--210055--?
UDP + H2OUMP + phosphate
show the reaction diagram
Kluyveromyces lactisQ9HEM6-488582--?
UDP + H2OUMP + phosphate
show the reaction diagram
Candida albicansQ8TGH6-655389--?
UDP + H2OUMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae-about 21% as active with UDP compared to GDP210057--?
UDP + H2OUMP + phosphate
show the reaction diagram
Bos taurus-much lower affinity than for GDP210052-210052?
UDP + H2OUMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae, Schizosaccharomyces pombe-about 10% as active with UDP compared to GDP210056--?
UDP + H2OUMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae, Schizosaccharomyces pombe-in the presence of 10 mM Mn2+: 3fold activation of its activity toward UDP, but not GDP, compared to activation by 10 mM Ca2+210056--?
UDP + H2OUMP + phosphate
show the reaction diagram
Kluyveromyces lactisQ9HEM6the enzyme's UDPase activity plays a role in uridine nucleotide sugar transport into Golgi vesicles488582--?
IDP + H2OIMP + phosphate
show the reaction diagram
Mus musculus, Homo sapiens, Mesocricetus auratus--210055--?
additional information?-Saccharomyces cerevisiae-substrate specificity210056, 210057---
additional information?-Bos taurus-substrate specificity210052---
additional information?-Schizosaccharomyces pombe-substrate specificity210056---
additional information?-Bos taurus-hydrolyzes free GDP, unable to hydrolyze GDP bound to eIF-2, eukaryotic initiation factor 2, and unable to catalyze the exchange of bound GDP with GTP even in the presence of relatively high molar excess of GTP and Met-tRNA210052---
additional information?-Saccharomyces cerevisiae-ADP: not a substrate210058---
additional information?-Bos taurus-only GDP and UDP are hydrolyzed, no activity with other nucleoside diphosphates and triphosphates, no activity with CDP, ADP, GTP, ATP210052---
additional information?-Saccharomyces cerevisiae-CDP: not a substrate, no or only very poor activity with other nucleotide substrates than GDP and UDP210057---
additional information?-Saccharomyces cerevisiae, Schizosaccharomyces pombe-less than 2% of maximum activity with other nucleoside di-, mono-, and triphosphates210056---
additional information?-Saccharomyces cerevisiae-guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen, GDPase is required to complete O-glycosylation of proteins, GDPase not essential for cell viability and growth, GDPase plays a role in O- and N-mannosylation of proteins such as chitinase and carboxypeptidase Y and glycosylation of external invertase and of mannosylation of inositolphosphorylceramides and in synthesis of cell wall210058---
additional information?-Saccharomyces cerevisiae-GDPase plays an important role in mannosylation of proteins and lipids by regulating the amount of GDP-mannose available in Golgi lumen210053, 210054, 210058---
additional information?-Saccharomyces cerevisiae-necessary for Golgi-specific glycosylation reactions210054---
additional information?-Saccharomyces cerevisiae-GDPase has an important role in glycosylation reactions in Golgi lumen210053, 210054, 210058---
additional information?-Saccharomyces cerevisiae-role in mannosylation210054, 210056, 210058---
additional information?-Kluyveromyces lactisQ9HEM6physiological function, GMP and UMP are required as antiporters for guanosine and uridine nucleotide sugar transport into the Golgi lumen, involved in O-glycosylation488582---
additional information?-Candida albicansQ8TGH6physiological function, role in O- and N-glycosylation, and cell and hyphae morphogenesis, affects the cell surface charge and phosphate content, enzyme is not required for the adhesion of Candida albicans to epithelial cells, overview655389---
additional information?-Schizosaccharomyces pombe-bifunctional enzyme showing GDPase and uridine diphosphatase activities in vitro655628---
additional information?-Kluyveromyces lactisQ9HEM6no activity with other nucleoside phosphates488582---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP + H2OGMP + phosphate
show the reaction diagram
Candida albicansQ8TGH6GDP hydrolysis occurs in the Golgi lumen to give GMP, which is required as antiporter for GDP-mannose transport into the Golgi lumen655389--?
GDP + H2OGMP + phosphate
show the reaction diagram
Schizosaccharomyces pombe-the enzyme has a main role in protein glycosylation, enzyme deficiency leads to N-glycosylation defects of invertase655628--?
GDP + H2OGMP + phosphate
show the reaction diagram
Kluyveromyces lactisQ9HEM6the enzyme's GDPase activity plays a role in guanosine nucleotide sugar transport into Golgi vesicles488582--?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae--210053-210053?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Bos taurus-possible biological function in maintaining the cellular pool of GTP-GDP210052-210052?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae-cleaves GDP that originates from GDP-mannose after mannosylation of glycoproteins in Golgi complex210054-210054?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae-GDP is a reaction product following outer-chain mannosylation of luminal proteins and inhibitor of mannosyltransferases. It is hypothesized that GMP then returns to the cytosol. GDPase presumably serves as an essential function in processing of yeast glycoproteins210056-210056?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae-GDPase plays a role in O- and N-mannosylation of proteins such as chitinase and carboxypeptidase Y and glycosylation of external invertase and of mannosylation of inositolphosphorylceramides210058-210058-
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae-GDP is known to be an inhibitor of mannosylation reactions in the Golgi lumen, removal of GDP by conversion to GMP, the latter not being an inhibitor of these reactions, would allow mannosylation to proceed210056-210056?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Saccharomyces cerevisiae-guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen210058-210058-
UDP + H2OUMP + phosphate
show the reaction diagram
Kluyveromyces lactisQ9HEM6the enzyme's UDPase activity plays a role in uridine nucleotide sugar transport into Golgi vesicles488582--?
GDP + H2O5'-GMP + phosphate
show the reaction diagram
Bos taurus-GTP is an important steric effector in many biological systems, GDP a potent inhibitor: GDPase activity could be involved in preventing potential inhibitory levels of GDP from accumulating210052-210052?
additional information?-Saccharomyces cerevisiae-guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen, GDPase is required to complete O-glycosylation of proteins, GDPase not essential for cell viability and growth, GDPase plays a role in O- and N-mannosylation of proteins such as chitinase and carboxypeptidase Y and glycosylation of external invertase and of mannosylation of inositolphosphorylceramides and in synthesis of cell wall210058---
additional information?-Saccharomyces cerevisiae-GDPase plays an important role in mannosylation of proteins and lipids by regulating the amount of GDP-mannose available in Golgi lumen210053, 210054, 210058---
additional information?-Saccharomyces cerevisiae-necessary for Golgi-specific glycosylation reactions210054---
additional information?-Saccharomyces cerevisiae-GDPase has an important role in glycosylation reactions in Golgi lumen210053, 210054, 210058---
additional information?-Saccharomyces cerevisiae-role in mannosylation210054, 210056, 210058---
additional information?-Kluyveromyces lactisQ9HEM6physiological function, GMP and UMP are required as antiporters for guanosine and uridine nucleotide sugar transport into the Golgi lumen, involved in O-glycosylation488582---
additional information?-Candida albicansQ8TGH6physiological function, role in O- and N-glycosylation, and cell and hyphae morphogenesis, affects the cell surface charge and phosphate content, enzyme is not required for the adhesion of Candida albicans to epithelial cells, overview655389---

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
Ca2+Saccharomyces cerevisiae-required for maximal activity210056, 210057
Ca2+Saccharomyces cerevisiae--210058
Ca2+Kluyveromyces lactisQ9HEM6stimulates both GDPase and UDPase activity488582
Ca2+Candida albicansQ8TGH6stimulates655389
Ca2+Schizosaccharomyces pombe-highly dependent on655628
Divalent cationsSaccharomyces cerevisiae-required210056, 210057
Fe2+Saccharomyces cerevisiae-less than 10% activation210056
Mg2+Saccharomyces cerevisiae-activation210056, 210057
Mg2+Schizosaccharomyces pombe-slight activation655628
Mn2+Saccharomyces cerevisiae-activation, enzyme with Mn2+ is 60-95% as active as compared with Ca2+; in the presence of 10 mM Mn2+: 3fold activation of its activity toward UDP, but not GDP, compared to activation by 10 mM Ca2+210056
Mn2+Kluyveromyces lactisQ9HEM6stimulates both GDPase and UDPase activity, stimulates UDPase activity by 5fold compared to stimulation with Ca2+488582
Mn2+Candida albicansQ8TGH6stimulates, preferred metal ion655389
Ni2+Saccharomyces cerevisiae-less than 10% activation210056
Zn2+Saccharomyces cerevisiae-less than 10% activation210056
Mn2+Schizosaccharomyces pombe-highly dependent on, best at 20 mM655628
additional informationSaccharomyces cerevisiae--210056

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
GDPBos taurus-hydrolysis of UDP210052 2D-image
UDPBos taurus-hydrolysis of GDP210052 2D-image

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
Triton X-100Saccharomyces cerevisiae-0.1% Triton X-100: 6-10fold activation210057 2D-image

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.1-GDPSaccharomyces cerevisiae--210056 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
3830-GDPSaccharomyces cerevisiae--210056 2D-image

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
3080-Saccharomyces cerevisiae--210056
4000-Bos taurus--210052
additional information-Bos taurus--210052
additional information-Kluyveromyces lactisQ9HEM6enzyme activity of wild-type and mutant enzyme in presence of Ca2+ or Mn2+ with substrates GDP or UDP488582
additional information-Candida albicansQ8TGH6-655389

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
7.48.2Saccharomyces cerevisiae-broad pH optimum210056
7.5-Bos taurus-assay at210052
7.5-Homo sapiens, Mesocricetus auratus, Mus musculus-assay at210055
7.5-Kluyveromyces lactisQ9HEM6assay at488582
7.5-Candida albicansQ8TGH6assay at655389
7.6-Saccharomyces cerevisiae-assay at210053, 210058

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
30-Kluyveromyces lactisQ9HEM6assay at488582
30-Candida albicansQ8TGH6assay at655389
37-Bos taurus-assay at210052
37-Homo sapiens, Mesocricetus auratus, Mus musculus-assay at210055

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
embryoMesocricetus auratus--210055Manually annotated by BRENDA team
fibroblastMesocricetus auratus--210055Manually annotated by BRENDA team
kidneyHomo sapiens--210055Manually annotated by BRENDA team
liverBos taurus--210052Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
Golgi apparatusSaccharomyces cerevisiae-distribution in multiple compartments; Golgi localization5794210053, 210054, 210057Manually annotated by BRENDA team
Golgi apparatusSaccharomyces cerevisiae-distribution in multiple compartments5794210058Manually annotated by BRENDA team
Golgi apparatusCandida albicansQ8TGH6-5794655389Manually annotated by BRENDA team
Golgi lumenSchizosaccharomyces pombe--5796210056Manually annotated by BRENDA team
Golgi lumenSaccharomyces cerevisiae-large catalytic lumenal domain5796210054, 210057Manually annotated by BRENDA team
Golgi lumenSaccharomyces cerevisiae--5796210053, 210056, 210058Manually annotated by BRENDA team
Golgi membraneSchizosaccharomyces pombe--139210056Manually annotated by BRENDA team
Golgi membraneSaccharomyces cerevisiae--139210053, 210056, 210057Manually annotated by BRENDA team
Golgi membraneKluyveromyces lactisQ9HEM6with the catalytic domain being lumenal139488582Manually annotated by BRENDA team
Golgi vesicleSchizosaccharomyces pombe-Golgi-like vesicles-210056Manually annotated by BRENDA team
Golgi vesicleSaccharomyces cerevisiae-Golgi-like vesicles-210056, 210057, 210058Manually annotated by BRENDA team
membraneSaccharomyces cerevisiae-type II transmembrane protein with a single hydrophobic domain, which acts as an uncleaved signal sequence and membrane anchor, and is preceded by a short hydrophilic cytosolic tail16020210053Manually annotated by BRENDA team
membraneSaccharomyces cerevisiae-type II integral membrane glycoprotein with a short amino-terminal cytoplasmic domain, which is not sufficient for Golgi localization, a single transmembrane domain , and a large catalytic lumenal domain, which is required for activity16020210054Manually annotated by BRENDA team
membraneSaccharomyces cerevisiae--16020210056, 210057Manually annotated by BRENDA team
membraneSaccharomyces cerevisiae-type II membrane protein with a single hydrophobic stretch acting as an uncleaved signal sequence and anchor to the membrane preceded by a short hydrophilic, cytosolic tail16020210058Manually annotated by BRENDA team
membraneSchizosaccharomyces pombe--16020210056, 655628Manually annotated by BRENDA team
microsomeBos taurus---210052Manually annotated by BRENDA team
ribosomeBos taurus--5840210052Manually annotated by BRENDA team
microsomeSchizosaccharomyces pombe---655628Manually annotated by BRENDA team
additional informationSaccharomyces cerevisiae-Golgi localization; subcellular localization-210054, 210057Manually annotated by BRENDA team
additional informationSaccharomyces cerevisiae-Golgi localization-210053, 210058Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
46000-Bos taurus-calf, gel filtration and glycerol density gradient centrifugation210052
47000-Saccharomyces cerevisiae-deglycosylated GDPase, result of proteolytic cleavage of the 55000 Da enzyme during purification, SDS-PAGE210053, 210056
48000-Saccharomyces cerevisiae-deglycosylated GDPase, result of proteolytic cleavage of the 57000 Da enzyme during purification, SDS-PAGE210058
55000-Saccharomyces cerevisiae--210053
55000-Saccharomyces cerevisiae-after signal sequence cleavage, metabolic labeling and immunoprecipitation, SDS-PAGE210054
56820-Saccharomyces cerevisiae-calculated from amino acid sequence210058
57000-Saccharomyces cerevisiae-before signal sequence cleavage, metabolic labeling and immunoprecipitation, SDS-PAGE210054
120000-Saccharomyces cerevisiae-functional target size, irradiation of membranes from wild-type and gda1 null mutants210053
additional information-Saccharomyces cerevisiae-determination of structural and functional target sizes of GDPase activity in situ210053
additional information-Saccharomyces cerevisiae--210054

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
dimerSaccharomyces cerevisiae-2 * 55000, guanosine diphosphatase functions in situ as a homodimer and does not require association with other membrane proteins for its function, immunodetection, SDS-PAGE210053
dimerCandida albicansQ8TGH6enzyme contains a unique sequence in the stem region predicted with high probability to adopt a coil-coil conformation that favors dimerization655389
monomerBos taurus-1 * 46000, calf, SDS-PAGE210052
additional informationSaccharomyces cerevisiae-guanosine diphosphatase functions in situ as a homodimer and does not require association with other membrane proteins for its function210053
additional informationSaccharomyces cerevisiae--210054

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
glycoproteinSaccharomyces cerevisiae-three potential N-glycosylation sites210053
glycoproteinSaccharomyces cerevisiae-N-linked oligosaccharides; three potential N-glycosylation sites210054
glycoproteinSaccharomyces cerevisiae--210056
glycoproteinSaccharomyces cerevisiae-high mannose glycoprotein; three potential N-glycosylation sites210058
proteolytic modificationSaccharomyces cerevisiae--210054
proteolytic modificationSaccharomyces cerevisiae-probably proteolytic cleavage of the enzyme during purification210058

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
No entries in this field

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
stable to several cycles of freezing and thawingSaccharomyces cerevisiae-210053
very stableSaccharomyces cerevisiae-210054

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-80°C, separated into aliquots, stable for a long timeSaccharomyces cerevisiae-210053

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
calfBos taurus-210052
normal and transforming PCPH proteins: PCPH, PCPH proto-oncogene and mt-PCPH, PCPH oncogene having guanosine diphosphatase activity, recombinant proteins from Escherichia coli BL21, DE3. Purified bacterial recombinant PCPH and mt-PCPH proteins have GDPase activityMesocricetus auratus-210055
-Saccharomyces cerevisiae-210056, 210058
partialSaccharomyces cerevisiae-210057
partialSchizosaccharomyces pombe-210056

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
DNA and amino acid sequence determination and analysis, functional complementation of a Saccharomyces GDPase null mutantCandida albicansQ8TGH6655389
normal and transforming PCPH proteins: PCPH, PCPH proto-oncogene and mt-PCPH, PCPH oncogene having guanosine diphosphatase activityHomo sapiens-210055
DNA sequence determination and analysis, enzyme can complement and restore activity in Saccharomyces cerevisiae deletion mutant gda1, complementation of N-glycosylation defectsKluyveromyces lactisQ9HEM6488582
normal and transforming PCPH proteins: PCPH, PCPH proto-oncogene and mt-PCPH, PCPH oncogene having guanosine diphosphatase activity, expression in Escherichia coli BL21, DE3Mesocricetus auratus-210055
normal and transforming PCPH proteins: PCPH, PCPH proto-oncogene and mt-PCPH, PCPH oncogene having guanosine diphosphatase activityMus musculus-210055
GDA1 geneSaccharomyces cerevisiae-210054
GDA1 gene; overexpression of GDA1 geneSaccharomyces cerevisiae-210058
overexpression in Schizosaccharomyces pombe, functional complementation of a Saccharomyces cerevisiae enzyme mutant defective in chitinase O-mannosylationSchizosaccharomyces pombe-655628

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
additional informationCandida albicansQ8TGH6disruption of gene gda1 leads to a mutant with 90% reduced GDP hydrolysis activity compared to the wild-type, the mutant also shows severely impaired O-mannosylation and reduced cell wall phosphate content and other phenotypes, but the formation of hyphae is uneffected, overview655389
additional informationKluyveromyces lactisQ9HEM6enzyme null mutants show 95% reduced activity in vitro and inhibition of glycosyltransferases utilizing uridine and guanosine nucleotide sugars due to accumulation of nucleoside diphosphates, the transport of both GDP-mannose and UDP-GlcNAc into the Golgi lumen is reduced in the null mutants, complementation of the disruption mutant by recombinant expression of the wild-type enzyme or the Saccharomyces cerevisiae enzyme488582
additional informationSchizosaccharomyces pombe-construction of s disruption mutant showing defects in N-glycosylation of other proteins655628

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

DISEASETITLE OF PUBLICATIONLINK TO PUBMED
Charcot-Marie-Tooth DiseaseA French family with Charcot-Marie-Tooth disease related to simultaneous heterozygous MFN2 and GDAP1 mutations. PubMed
Charcot-Marie-Tooth DiseaseA novel autosomal dominant GDAP1 mutation in an Italian CMT2 family. PubMed
Charcot-Marie-Tooth DiseaseA novel GDAP1 Q218E mutation in autosomal dominant Charcot-Marie-Tooth disease. PubMed
Charcot-Marie-Tooth DiseaseA novel mutation in the GDAP1 gene is associated with autosomal recessive Charcot-Marie-Tooth disease in an Amish family. PubMed
Charcot-Marie-Tooth DiseaseA novel mutation of GDAP1 associated with Charcot-Marie-Tooth disease in three Italian families: evidence for a founder effect. PubMed
Charcot-Marie-Tooth DiseaseAutosomal recessive axonal form of Charcot-Marie-Tooth Disease caused by compound heterozygous 3'-splice site and Ser130Cys mutation in the GDAP1 gene. PubMed
Charcot-Marie-Tooth DiseaseCharcot-Marie-Tooth disease CMT4A: GDAP1 increases cellular glutathione and the mitochondrial membrane potential. PubMed
Charcot-Marie-Tooth DiseaseCharcot-Marie-Tooth disease type 4C4 caused by a novel Pro153Leu substitution in the GDAP1 gene. PubMed
Charcot-Marie-Tooth DiseaseCharcot-Marie-Tooth disease-associated mutants of GDAP1 dissociate its roles in peroxisomal and mitochondrial fission. PubMed
Charcot-Marie-Tooth DiseaseCharcot-Marie-Tooth-related gene GDAP1 complements cell cycle delay at G2/M phase in Saccharomyces cerevisiae fis1 gene-defective cells. PubMed
Charcot-Marie-Tooth DiseaseDominant GDAP1 founder mutation is a common cause of axonal Charcot-Marie-Tooth disease in Finland. PubMed
Charcot-Marie-Tooth DiseaseEarly onset Charcot-Marie-Tooth disease caused by a homozygous Leu239Phe mutation in the GDAP1 gene. PubMed
Charcot-Marie-Tooth DiseaseEvolutionary and structural analyses of GDAP1, involved in Charcot-Marie-Tooth disease, characterize a novel class of glutathione transferase-related genes. PubMed
Charcot-Marie-Tooth DiseaseGanglioside-induced differentiation associated protein 1 is a regulator of the mitochondrial network: new implications for Charcot-Marie-Tooth disease. PubMed
Charcot-Marie-Tooth DiseaseGDAP1, the protein causing Charcot-Marie-Tooth disease type 4A, is expressed in neurons and is associated with mitochondria. PubMed
Charcot-Marie-Tooth DiseaseIdentification of novel GDAP1 mutations causing autosomal recessive Charcot-Marie-Tooth disease. PubMed
Charcot-Marie-Tooth DiseaseL239F founder mutation in GDAP1 is associated with a mild Charcot-Marie-Tooth type 4C4 (CMT4C4) phenotype. PubMed
Charcot-Marie-Tooth DiseaseMitochondrial complex I deficiency in GDAP1-related autosomal dominant Charcot-Marie-Tooth disease (CMT2K). PubMed
Charcot-Marie-Tooth DiseaseMitochondrial dysfunction and pathophysiology of Charcot-Marie-Tooth disease involving GDAP1 mutations. PubMed
Charcot-Marie-Tooth DiseaseNovel human pathological mutations. Gene symbol: GDAP1. Disease: Charcot-Marie-Tooth disease 4A. PubMed
Charcot-Marie-Tooth DiseasePhenotypical features of a Moroccan family with autosomal recessive Charcot-Marie-Tooth disease associated with the S194X mutation in the GDAP1 gene. PubMed
Charcot-Marie-Tooth DiseasePhenotypical features of the p.R120W mutation in the GDAP1 gene causing autosomal dominant Charcot-Marie-Tooth disease. PubMed
Charcot-Marie-Tooth DiseaseThe Gdap1 knockout mouse mechanistically links redox control to Charcot-Marie-Tooth disease. PubMed
Charcot-Marie-Tooth DiseaseTwo novel mutations in the GDAP1 and PRX genes in early onset Charcot-Marie-Tooth syndrome. PubMed
Charcot-Marie-Tooth DiseaseVocal cord and diaphragm paralysis, as clinical features of a French family with autosomal recessive Charcot-Marie-Tooth disease, associated with a new mutation in the GDAP1 gene. PubMed
Charcot-Marie-Tooth DiseaseYY1-dependent transcriptional regulation of the human GDAP1 gene. PubMed
ClubfootMutations in the ganglioside-induced differentiation-associated protein-1 (GDAP1) gene in intermediate type autosomal recessive Charcot-Marie-Tooth neuropathy. PubMed
Congenital AbnormalitiesMutations in the ganglioside-induced differentiation-associated protein-1 (GDAP1) gene in intermediate type autosomal recessive Charcot-Marie-Tooth neuropathy. PubMed
Demyelinating DiseasesA locus-specific database for mutations in GDAP1 allows analysis of genotype-phenotype correlations in Charcot-Marie-Tooth diseases type 4A and 2K. PubMed
Demyelinating DiseasesGanglioside-induced differentiation associated protein 1 is a regulator of the mitochondrial network: new implications for Charcot-Marie-Tooth disease. PubMed
Demyelinating DiseasesMutations in GDAP1: autosomal recessive CMT with demyelination and axonopathy. PubMed
Demyelinating DiseasesMutations in the ganglioside-induced differentiation-associated protein-1 (GDAP1) gene in intermediate type autosomal recessive Charcot-Marie-Tooth neuropathy. PubMed
Foot DeformitiesGenetic spectrum of hereditary neuropathies with onset in the first year of life. PubMed
Hereditary Sensory and Motor NeuropathyGenetic study of demyelinating form of autosomal-recessive Charcot-Marie-tooth diseases in a Turkish family. PubMed
Hereditary Sensory and Motor Neuropathy[Molecular genetics of inherited neuropathies]. PubMed,  PubMed
HoarsenessCharcot-Marie-Tooth disease type 4C4 caused by a novel Pro153Leu substitution in the GDAP1 gene. PubMed
Mitochondrial DiseasesA locus-specific database for mutations in GDAP1 allows analysis of genotype-phenotype correlations in Charcot-Marie-Tooth diseases type 4A and 2K. PubMed
Muscle WeaknessTwo novel mutations in the GDAP1 and PRX genes in early onset Charcot-Marie-Tooth syndrome. PubMed
NeoplasmsENTPD5, an endoplasmic reticulum UDPase, alleviates ER stress induced by protein overloading in AKT-activated cancer cells. PubMed
NeoplasmsENTPD5-mediated modulation of ATP results in altered metabolism and decreased survival in gliomablastoma multiforme. PubMed
NeoplasmsYY1-dependent transcriptional regulation of the human GDAP1 gene. PubMed
NeuroblastomaGDAP1, the protein causing Charcot-Marie-Tooth disease type 4A, is expressed in neurons and is associated with mitochondria. PubMed
Neurodegenerative DiseasesMitochondrial dysfunction and pathophysiology of Charcot-Marie-Tooth disease involving GDAP1 mutations. PubMed
OphthalmoplegiaInherited mitochondrial neuropathies. PubMed
Ovarian NeoplasmsSialyltransferase and nucleoside diphosphatase as markers for tumor monitoring. PubMed
ParalysisClinical, electrophysiological and morphological findings of Charcot-Marie-Tooth neuropathy with vocal cord palsy and mutations in the GDAP1 gene. PubMed
ParalysisVocal cord and diaphragm paralysis, as clinical features of a French family with autosomal recessive Charcot-Marie-Tooth disease, associated with a new mutation in the GDAP1 gene. PubMed
ParesisThe gene encoding ganglioside-induced differentiation-associated protein 1 is mutated in axonal Charcot-Marie-Tooth type 4A disease. PubMed
Peripheral Nervous System DiseasesCell expression of GDAP1 in the nervous system and pathogenesis of Charcot-Marie-Tooth type 4A disease. PubMed
Peripheral Nervous System DiseasesCharcot-Marie-Tooth disease CMT4A: GDAP1 increases cellular glutathione and the mitochondrial membrane potential. PubMed
Peripheral Nervous System DiseasesCharcot-Marie-Tooth disease-associated mutants of GDAP1 dissociate its roles in peroxisomal and mitochondrial fission. PubMed
Peripheral Nervous System DiseasesGDAP1 mutations differ in their effects on mitochondrial dynamics and apoptosis depending on the mode of inheritance. PubMed
Peripheral Nervous System DiseasesMitochondrial dysfunction and pathophysiology of Charcot-Marie-Tooth disease involving GDAP1 mutations. PubMed
Peripheral Nervous System DiseasesThe Gdap1 knockout mouse mechanistically links redox control to Charcot-Marie-Tooth disease. PubMed
PolyneuropathiesInherited mitochondrial neuropathies. PubMed
Respiratory InsufficiencyVocal cord paresis and diaphragmatic dysfunction are severe and frequent symptoms of GDAP1-associated neuropathy. PubMed
Vocal Cord ParalysisClinical, electrophysiological and morphological findings of Charcot-Marie-Tooth neuropathy with vocal cord palsy and mutations in the GDAP1 gene. PubMed
Vocal Cord ParalysisThe gene encoding ganglioside-induced differentiation-associated protein 1 is mutated in axonal Charcot-Marie-Tooth type 4A disease. PubMed

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISM (UNIPROT ACCESSION NO.)LINK TO PUBMEDSOURCE
210052Raychaudhuri, P.; Ghosh, S.; Maitra, U.Purification and characterization of a guanosine diphosphatase activity from calf liver microsomal salt wash proteinsJ. Biol. Chem.2608306-83111985Bos taurus PubMed
210053Berninsone, P.; Lin, Z.Y.; Kempner, E.; Hirschberg, C.B.Regulation of yeast Golgi glycosylation. Guanosine diphosphatase functions as a homodimer in the membraneJ. Biol. Chem.27014564-145671995Saccharomyces cerevisiae PubMed
210054Vowels, J.J.; Payne, G.S.A role for the lumenal domain in Golgi localization of the Saccharomyces cerevisiae guanosine diphosphataseMol. Biol. Cell91351-13651998Saccharomyces cerevisiae PubMed
210055Recio, J.A.; Paez, J.G.; Maskeri, B.; Loveland, M.; Velasco, J.A.; Notario, V.Both normal and transforming PCPH proteins have guanosine diphosphatase activity but only the oncoprotein cooperates with ras in activating extracellular signal-regulated kinase ERK1Cancer Res.601720-17282000Homo sapiens, Mesocricetus auratus, Mus musculus PubMed
210056Yanagisawa, K.; Resnick, D.; Abeijon, C.; Robbins, P.W.; Hirschberg, C.B.A guanosine diphosphatase enriched in Golgi vesicles of Saccharomyces cerevisiae. Purification and characterizationJ. Biol. Chem.26519351-193551990Saccharomyces cerevisiae, Schizosaccharomyces pombe PubMed
210057Abeijon, C.; Orlean, P.; Robbins, P.W.; Hirschberg, C.B.Topography of glycosylation in yeast: characterization of GDPmannose transport and lumenal guanosine diphosphatase activities in Golgi-like vesiclesProc. Natl. Acad. Sci. USA866935-69391989Saccharomyces cerevisiae PubMed
210058Abeijon, C.; Yanagisawa, K.; Mandon, E.C.; Häusler, A.; Moremen, K.; Hirschberg, C.B.; Robbins, P.W.Guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen of Saccharomyces cerevisiaeJ. Cell Biol.122307-3231993Saccharomyces cerevisiae PubMed
488582Lopez-Avalos, M.D.; Uccelletti, D.; Abeijon, C.; Hirschberg, C.B.The UDPase activity of the Kluyveromyces lactis Golgi GDPase has a role in uridine nucleotide sugar transport into Golgi vesiclesGlycobiology11413-4222001Kluyveromyces lactis, Kluyveromyces lactis (Q9HEM6) PubMed
655389Herrero, A.B.; Uccelletti, D.; Hirschberg, C.B.; Dominguez, A.; Abeijon, C.The Golgi GDPase of the fungal pathogen Candida albicans affects morphogenesis, glycosylation, and cell wall propertiesEukaryot. Cell1420-4312002Candida albicans, Candida albicans (Q8TGH6) PubMed
655628Sanchez, R.; Franco, A.; Gacto, M.; Notario, V.; Cansado, J.Characterization of gdp1+ as encoding a GDPase in the fission yeast Schizosaccharomyces pombeFEMS Microbiol. Lett.22833-382003Schizosaccharomyces pombe PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 3.6.1.42)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)