Information on EC 3.1.11.6 -

Information on EC 3.1.11.6 - exodeoxyribonuclease VII:

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EC NUMBER	COMMENTARY
3.1.11.6	-

RECOMMENDED NAME	GeneOntology No.
exodeoxyribonuclease VII	GO:0009318

REACTION	REACTION DIAGRAM	COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates		-	-	-	-

REACTION TYPE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
hydrolysis of phosphoric ester	-	-	-	-

PATHWAY	KEGG Link	MetaCyc Link
No entries in this field

SYSTEMATIC NAME	IUBMB Comments
No entries in this field

SYNONYMS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
E. coli exonuclease VII	-	-	-	-
endodeoxyribonuclease VII	-	-	-	-
Escherichia coli exonuclease VII	-	-	-	-
exonuclease VII	-	-	-	-
exonuclease VII	Escherichia coli	-	-	665060, 680517, 700496, 707510, 715119
exonuclease VII	Thermotoga maritima	-	-	700496
ExoVII	Escherichia coli	-	-	680517, 682560, 700327, 715119
ExoVII	Escherichia coli K-12	-	-	698618
ExoVII	Haemophilus influenzae	-	-	700329
nuclease, exodeoxyribo-, VII	-	-	-	-
RecBCD	Escherichia coli	-	-	665060
XseA	Thermotoga maritima	-	-	700496
XseB	Thermotoga maritima	-	-	700496

CAS REGISTRY NUMBER	COMMENTARY
52933-20-7	-

ORGANISM	COMMENTARY	LITERATURE	SEQUENCE CODE	SEQUENCE DB	SOURCE
Escherichia coli	-	134040, 134045, 134046, 652155, 653673, 680517, 700496, 707510, 715119	-	-
Escherichia coli	gene recD	665060	-	-
Escherichia coli	numerous mutants; xseA	134036	-	-
Escherichia coli	strain AB1157	682560	-	-
Escherichia coli	strain BW25113	700327	-	-
Escherichia coli	xseA	134035, 134037, 134038, 134039, 134041, 134042, 134043, 134048	-	-
Escherichia coli	xseA	134047	P04994	Uniprot
Escherichia coli	xseB	134044	-	-
Escherichia coli AB1157	strain AB1157	682560	-	-
Escherichia coli K-12	strain ZK126	698618	-	-
Escherichia coli K-12 ZK126	strain ZK126	698618	-	-
Escherichia coli K-12, MG1655	-	715119	-	-
Escherichia coli xseA	xseA	134035	-	-
Escherichia coli xseA	xseA	134047	P04994	-
Escherichia coli xseB	xseB	134044	-	-
Haemophilus influenzae	strain RM118	700329	-	-
Haemophilus influenzae RM118	strain RM118	700329	-	-
Thermotoga maritima	-	700496	-	-

GENERAL INFORMATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
malfunction	Escherichia coli	-	ExoI- ExoVII- SbcCD- cells are sensitive to mitomycin C and 2-aminopurine. Cells lacking all three nucleases, exonuclease I, exonuclease VII, or SbcCD, are inviable without RecG. Removing RNase HI mimics the effect of removing RecG in that DrnhA cells are inviable if ExoI, ExoVII, and SbcCD are eliminated	715119
physiological function	Escherichia coli	-	mismatch repair proteins MutS and UvrD stimulate single-stranded DNA-specific exonuclease VII action on double-stranded DNA substrates. MutH and MutL proteins show no stimulation of exonuclease VII action. UvrD-mediated stimulation of exonuclease VII action on double-stranded DNA is not accompanied by strand separation. UvrD-mediated stimulation of exonuclease VII action on double-stranded DNA is accentuated by MutS and SSB proteins but not by MutL	707510
physiological function	Escherichia coli	-	to stay alive, cells must have either RecG or a 3'-single-stranded DNA exonuclease, which can be exonuclease I, exonuclease VII, or SbcCD. Cells lacking all three nucleases are inviable without RecG	715119

SUBSTRATE	PRODUCT	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY/ Substrate	LITERATURE/ Substrate	COMMENTARY/ Product	LITERATURE/ Product	Reversibility r=reversible ir=irreversible ?=not specified
duplex DNA containing single-stranded termini + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134037	-	134037	?
polydeoxyribonucleotides + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134035	-	134035	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134039	-	134039	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134040	-	134040	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134041	-	134041	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134042	-	134042	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134043	-	134043	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134044	-	134044	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134045	-	134045	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134046	-	134046	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	P04994	-	134047	-	134047	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134048	-	134048	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	680517, 682560, 707510	-	-	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134035	oligonucleotides	134035	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134036	oligonucleotides	134036	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134037	oligonucleotides	134037	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134038	oligonucleotides	134038	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	no activity on circular DNA	134037	exclusively oligonucleotides	134037	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	no activity on circular DNA	134038	exclusively oligonucleotides, no mononucleotide production observed	134038	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	no endonuclease activity, no activity homopolymers	134035	limit products: oligonucleotides: dimers to dodecamers	134035	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	processive degradation	134036	-	134036	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	processive degradation	134035	limit products: oligonucleotides: dimers to dodecamers	134035	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	processive degradation	134037	exclusively oligonucleotides	134037	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	processive degradation	134038	exclusively oligonucleotides, no mononucleotide production observed	134038	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	no activity on DNA-RNA hybrid molecules	134037	exclusively oligonucleotides	134037	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	no activity on DNA-RNA hybrid molecules	134038	exclusively oligonucleotides, no mononucleotide production observed	134038	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	single stranded DNA of at least 10-20 nucleotides are required for maximal activity	134038	exclusively oligonucleotides, no mononucleotide production observed	134038	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	heat-denatured DNA, no activity on RNA	134037	exclusively oligonucleotides	134037	?
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	no activity on RNA	134038	exclusively oligonucleotides, no mononucleotide production observed	134038	?
single-stranded DNA + H2O	?		Escherichia coli K-12	-	-	698618	-	-	?
single-stranded DNA + H2O	?		Thermotoga maritima	-	ExoVII activity is highly processive. It binds ssDNA ends and cleaves its substrate endonucleolytically, releasing oligonucleotide products of various sizes	700496	-	-	?
single-stranded DNA + H2O	?		Escherichia coli	-	releases oligonucleotide products	700496	-	-	?
thymine dimers + H2O	hydrolyzed single-stranded DNA		Escherichia coli	P04994	possible role in methyl-directed mismatch repair system	134047	-	134047	?
thymine dimers + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	possible role in methyl-directed mismatch repair system	134048	-	134048	?
thymine dimers + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	possible role in repair of ultraviolet damage	134035	-	134035	?
thymine dimers + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	possible role in repair of ultraviolet damage	134036	-	134036	?
thymine dimers + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	possible role in repair of ultraviolet damage	134037	-	134037	?
thymine dimers + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	possible role in repair of ultraviolet damage	134038	-	134038	?
thymine dimers + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	possible role in repair of ultraviolet damage	134039	-	134039	?
thymine dimers + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	possible role in repair of ultraviolet damage	134041	-	134041	?
duplex DNA containing single-stranded termini + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134038	-	134038	?
additional information	?	-	Escherichia coli	-	involved in methyl-directed mismatch repair	653673	-	-	-
additional information	?	-	Escherichia coli	-	RecBCD subunit RecD is required for initiation of homologous recombination in Escherichia coli and essential for phage lambda recombination, in contrast to exonuclease RecJ, overview	665060	-	-	-
additional information	?	-	Haemophilus influenzae	-	ExoVII is refractory to mutation, inactivation appears to be lethal. ExoVII appears to be essential for growth of Haemophilus influenzae, possibly, given its ability to process DNA flaps, indicating a requirement for the activities of this protein in replicative DNA synthesis	700329	-	-	-
additional information	?	-	Escherichia coli K-12	-	mutants lacking ExoVII are viable during all phases of the bacterial life cycle, but cannot compete with wild-type cells during long-term stationary phase incubation. Mutants lacking ExoVII are defective in DNA catabolism	698618	-	-	-
additional information	?	-	Thermotoga maritima	-	active site motif with two conserved aspartate residues in the large (XseA/TM1768) subunit. Residues Asp235 and Asp240 are essential for the nuclease activity, no significant activity on dsDNA. Active site motif with two conserved aspartate residues in the large (XseA/TM1768) subunit. Residues Asp235 and Asp240 are essential for the nuclease activity	700496	-	-	-
additional information	?	-	Escherichia coli	-	exonuclease VII can digest ssDNA from 3' ends	715119	-	-	-

NATURAL SUBSTRATES	NATURAL PRODUCTS	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY SUBSTRATE	LITERATURE (Substrate)	COMMENTARY PRODUCT	LITERATURE (Product)
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134035	oligonucleotides	134035
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134036	oligonucleotides	134036
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134037	oligonucleotides	134037
single-stranded DNA + H2O	hydrolyzed single-stranded DNA		Escherichia coli	-	-	134038	oligonucleotides	134038
additional information	?	-	Escherichia coli	-	involved in methyl-directed mismatch repair	653673	-	-
additional information	?	-	Escherichia coli	-	RecBCD subunit RecD is required for initiation of homologous recombination in Escherichia coli and essential for phage lambda recombination, in contrast to exonuclease RecJ, overview	665060	-	-
additional information	?	-	Haemophilus influenzae	-	ExoVII is refractory to mutation, inactivation appears to be lethal. ExoVII appears to be essential for growth of Haemophilus influenzae, possibly, given its ability to process DNA flaps, indicating a requirement for the activities of this protein in replicative DNA synthesis	700329	-	-
additional information	?	-	Escherichia coli K-12	-	mutants lacking ExoVII are viable during all phases of the bacterial life cycle, but cannot compete with wild-type cells during long-term stationary phase incubation. Mutants lacking ExoVII are defective in DNA catabolism	698618	-	-
additional information	?	-	Escherichia coli	-	exonuclease VII can digest ssDNA from 3' ends	715119	-	-

COFACTOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

METALS and IONS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
KCl	Thermotoga maritima	-	ExoVII activity is maximal at low salt concentrations (lower than 250 mM)	700496
Mg2+	Escherichia coli	-	25% activation	134037
Mg2+	Thermotoga maritima	-	robust nuclease activity at 80�C is magnesium dependent	700496
additional information	Thermotoga maritima	-	manganese, nickel, cobalt and zinc have no effect	700496

INHIBITORS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
EDTA	Escherichia coli	-	can funtion in absence of divalent cations	134035	2D-image
EDTA	Escherichia coli	-	can funtion in absence of divalent cations; slight inhibition	134037	2D-image
N-ethylmaleimide	Escherichia coli	-	47% inhibition in absence of mercaptoethanol	134037	2D-image
phosphate	Thermotoga maritima	-	inhibits nuclease activity at 80�C; strongly inhibits, almost complete inhibition by 50 mM	700496	2D-image
sulfate	Thermotoga maritima	-	strongly inhibits, almost complete inhibition by 50 mM	700496	2D-image
EDTA	Thermotoga maritima	-	; completely inhibits	700496	2D-image
additional information	Thermotoga maritima	-	is not active in standard Escherichia coli ExoVII buffer. Acetate has no effect	700496	-

ACTIVATING COMPOUND	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
phosphate	Escherichia coli	-	5-10fold higher activity compared to Tris-HCL buffer	134037	2D-image
phosphate	Escherichia coli	-	stimulates	700496	2D-image
sulfate	Escherichia coli	-	stimulates	700496	2D-image
2-mercaptoethanol	Escherichia coli	-	required for 100% activity	134037	2D-image
additional information	Escherichia coli	-	becomes activated upon DNA damage by single-stranded DNA regions, in order to confer better opportunities for cell survival	700327	-
additional information	Escherichia coli	-	acetate has no effect	700496	-

KM VALUE [mM]	KM VALUE [mM] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

TURNOVER NUMBER [1/s]	TURNOVER NUMBER MAXIMUM[1/s]	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

kcat/KM VALUE [1/mMs^-1]	kcat/KM VALUE [1/mMs^-1] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

Ki VALUE [mM]	Ki VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

IC50 VALUE [mM]	IC50 VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg]	SPECIFIC ACTIVITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
221	-	Thermotoga maritima	-	-	700496
267	-	Escherichia coli	-	-	700496

pH OPTIMUM	pH MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
7.8	7.9	Escherichia coli	-	-	134037
7.9	-	Thermotoga maritima	-	-	700496

pH RANGE	pH RANGE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

TEMPERATURE OPTIMUM	TEMPERATURE OPTIMUM MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
37	-	Escherichia coli	-	assay	134037
80	-	Thermotoga maritima	-	XseA/B homologs TM1768 and TM1769 show robust and maximal nuclease activity	700496

TEMPERATURE RANGE	TEMPERATURE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

pI VALUE	pI VALUE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

SOURCE TISSUE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	SOURCE
No entries in this field

LOCALIZATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	GeneOntology No.	LITERATURE	SOURCE
cytoplasm	Escherichia coli K-12	-	-	5737	698618

PDB	SCOP	CATH	ORGANISM
1vp7, download	SCOP (1vp7)	CATH (1vp7)	Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253)

MOLECULAR WEIGHT	MOLECULAR WEIGHT MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
51820	-	Escherichia coli	-	large subunit, amino acid sequence	134046
88000	-	Escherichia coli	-	gel fltration	134037

SUBUNITS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
pentamer	Escherichia coli	-	heterodimer, 1 * 54000, 4 * 105000, large subunit: xseA gene, small subunit: xseB gene, SDS-PAGE	134042, 134044
additional information	Thermotoga maritima	-	stoichiometry of small to large subunits regulates ExoVII activity	700496

POSTTRANSLATIONAL MODIFICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

Crystallization/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

pH STABILITY	pH STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
7.3	7.8	Escherichia coli	-	50% loss of activity compared to pH 7.8	134037
7.8	8.6	Escherichia coli	-	50% loss of activity compared to pH 7.8	134037

TEMPERATURE STABILITY	TEMPERATURE STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
37	-	Escherichia coli	-	half-life: 90 min	134037
45	-	Escherichia coli	-	half-life: 45 min	134037
60	-	Escherichia coli	-	half-life: 7 min	134037
80	-	Thermotoga maritima	-	XseA/B homologs TM1768 and TM1769 show robust nuclease activity	700496

GENERAL STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
freezing and thawing causes loss of activity	Escherichia coli	-	134036

ORGANIC SOLVENT	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

OXIDATION STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

STORAGE STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
-20�C, 20 mM Tris-HCl, pH 8, 10 mM mercaptoethanol, 0.1 mM EDTA, 0.2 M NaCl, 50% glycerol, 18 months, 10% loss of activity	Escherichia coli	-	134036

Purification/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
1700fold	Escherichia coli	-	134036, 134037
7500fold to 87% homogeneity	Escherichia coli	-	134042
wild-type and mutant ExoVII (XseA/B homologs TM1768 and TM1769) purified by gel filtration and on nickel-NTA column; wild-type and mutant ExoVII (XseA/B homologs TM1768 and TM1769) purified on nickel-NTA column	Thermotoga maritima	-	700496

Cloned/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
large subunit	Escherichia coli	-	134045
strain deficient in enzymic activity plus deficiency in RecJ, ExoI and ExoX exonuclease activities as well as a strain deficient in RecJ, ExoVII and ExoI exonucleases grow poorly in presence of 2-aminopurine. Exposure to 2-aminopurine results in filament formation. The quadruple mutant is cold-sensitive with a severe growth defect at 30�C	Escherichia coli	-	653673
strain deficient in enzymic activity plus deficiency in RecJ, ExoI and ExoX exonuclease activities displays a 7fold increase in mutation rate, the availabilty of any one of thr nucleases is enough to support full mismatch correction	Escherichia coli	-	652155
wild-type and mutant ExoVII (XseA/B homologs TM1768 and TM1769) coexpressed in Escherichia coli BL21(DE3) cells	Thermotoga maritima	-	700496

EXPRESSION	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

ENGINEERING	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
D235A/D240A	Thermotoga maritima	-	is completely inactive, even in the presence of magnesium	700496
additional information	Escherichia coli	-	mutants lacking the xseA gene	134047, 134048
additional information	Escherichia coli	-	construction of recD mutants showing a RecBC enzyme, instead of a RecBCD enzyme, lacking the RecD subunit and exonuclease VII activity, the mutation affects phage lambda red gam recombination, but does not cause an altered phenotype, overview	665060
additional information	Escherichia coli	-	recJ xseA sbcCD xonA2 quadruple mutants are cold sensitive depending on the xon allele. Absence of the two 3' exonucleases ExoI and ExoVII allows highly efficient conjugational recombination in recBCD+ cells, which is independent of SbcCD, recJ xseA double mutant has lower UV-survival than wild-type	680517
additional information	Escherichia coli	-	mutant in all four exonucleases (ExoI, ExoVII, ExoX, and RecJ) exhibits pronounced 20fold elevation of cross-overs between 25 bp of homology relative to the wild-type	682560
additional information	Escherichia coli	-	conbstruction of ExoVII-deficient cells and of cells lacking alll three exonuxleases, exonuclease I, exonuclease VII, or SbcCD, phenotypes, detailed overview	715119

Renatured/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

APPLICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
analysis	Escherichia coli	-	degradation of adenovirus-associated DNA; excision of DNA segments from cloning vectors; of nucleic acid structure; of spliced RNA; removal of single-stranded DNA	134036
analysis	Escherichia coli	-	purification of DNA rings	134040
analysis	Escherichia coli	-	xseA-mutants for measurement of single-stranded apurinic-apyrimidinic endonuclease	134043
analysis	Escherichia coli	-	mapping of mRNA isoforms	134046
additional information	Escherichia coli	-	ExoVII, encoded by xseA, has an antirecombination function	680517
additional information	Escherichia coli	-	RecA-independent recombination is depressed by the redundant action of single-strand exonucleases. ExoVII inhibits crossing-over	682560

REF.	AUTHORS	TITLE	JOURNAL	VOL.	PAGES	YEAR	ORGANISM	LINK TO PUBMED	SOURCE
134035	Chase, J.W.; Richardson, C.C.	Exonuclease VII of Escherichia coli	Basic Life Sci.	5A	225-234	1975	Escherichia coli	PubMed
134036	Chase, J.W.; Vales, L.D.	Exonuclease VII of E. coli	Gene Amplif. Anal.	2	147-168	1981	Escherichia coli	PubMed
134037	Chase, J.W.; Richardson, C.C.	Exonuclease VII of Escherichia coli. Purification and properties	J. Biol. Chem.	249	4545-4552	1974	Escherichia coli	PubMed
134038	Chase, J.W.; Richardson, C.C.	Exonuclease VII of Escherichia coli. Mechanism of action	J. Biol. Chem.	249	4553-4561	1974	Escherichia coli	PubMed
134039	Chase, J.W.; Masker, W.E.	Deoxyribonucleic acid repair in Escherichia coli mutants deficient in the 5'-3' exonuclease activity of deoxyribonucleic acid polymerase I and exonuclease VII	J. Bacteriol.	130	667-675	1977	Escherichia coli	PubMed
134040	Hutton, J.R.; Thomas, C.A.	The assay and isolation of DNA rings using an ATP-dependent endonuclease	Biochemistry	14	1432-1436	1975	Escherichia coli	PubMed
134041	Chase, J.W.; Masker, W.E.; Murphy, J.B.	Pyrimidine dimer excision in Escherichia coli strains deficient in exonucleases V and VII and in the 5' leads to 3' exonuclease of DNA polymerase I	J. Bacteriol.	137	234-242	1979	Escherichia coli	PubMed
134042	Vales, L.D.; Rabin, B.A.; Chase, J.W.	Subunit structure of Escherichia coli exonuclease VII	J. Biol. Chem.	257	8799-8805	1982	Escherichia coli	PubMed
134043	Schultz, R.A.; Friedberg, E.C.; Moses, R.E.; Rupp, W.D.; Sancar, A.; Sharma, S.	Use of an Escherichia coli mutant strain permits measurement of single-stranded apurinic-apyrimidinic endonuclease in crude extracts: studies with untransformed cells and cells transformed with plasmids containing the uvrC gene	J. Bacteriol.	154	1459-1461	1983	Escherichia coli	PubMed
134044	Vales, L.D.; Rabin, B.A.; Chase, J.W.	Isolation and preliminary characterization of Escherichia coli mutants deficient in exonuclease VII	J. Bacteriol.	155	1116-1122	1983	Escherichia coli	PubMed
134045	Chase, J.W.; Rabin, B.A.; Murphy, J.B.; Stone, K.L.; Williams, K.R.	Escherichia coli exonuclease VII. Cloning and sequencing of the gene encoding the large subunit (xseA)	J. Biol. Chem.	261	14929-14935	1986	Escherichia coli	PubMed
134046	Chassagne, C.; Schwartz, K.	Mapping of mRNA isoforms with an oligonucleotide probe: exonuclease VII compared with endonucleases	Nucleic Acids Res.	20	3256	1992	Escherichia coli	PubMed
134047	Harris, R.S.; Ross, K.J.; Lombardo, M.J.; Rosenberg, S.M.	Mismatch repair in Escherichia coli cells lacking single-strand exonucleases ExoI, ExoVII, and RecJ	J. Bacteriol.	180	989-993	1998	Escherichia coli	PubMed
134048	Viswanathan, M.; Lovett, S.T.	Single-strand DNA-specific exonucleases in Escherichia coli. Roles in repair and mutation avoidance	Genetics	149	7-16	1998	Escherichia coli	PubMed
652155	Viswanathan, M.; Burdett, V.; Baitinger, C.; Modrich, P.; Lovett, S.T.	Redundant exonuclease involvement in Escherichia coli methyl-directed mismatch repair	J. Biol. Chem.	276	31053-31058	2001	Escherichia coli	PubMed
653673	Burdett, V.; Baitinger, C.; Viswanathan, M.; Lovett, S.T.; Modrich, P.	In vivo requirement for RecJ, ExoVII, ExoI, and ExoX in methyl-directed mismatch repair	Proc. Natl. Acad. Sci. USA	98	6765-6770	2001	Escherichia coli	PubMed
665060	Dermic, D.; Zahradka, D.; Petranovic, M.	Exonuclease requirements for recombination of lambda phage in recD mutants of Escherichia coli	Genetics	173	2399-2402	2006	Escherichia coli	PubMed
680517	Thoms, B.; Borchers, I.; Wackernagel, W.	Effects of single-strand DNases ExoI, RecJ, ExoVII, and SbcCD on homologous recombination of recBCD+ strains of Escherichia coli and roles of SbcB15 and XonA2 ExoI mutant enzymes	J. Bacteriol.	190	179-192	2008	Escherichia coli	PubMed
682560	Dutra, B.E.; Sutera, V.A.; Lovett, S.T.	RecA-independent recombination is efficient but limited by exonucleases	Proc. Natl. Acad. Sci. USA	104	216-221	2007	Escherichia coli	PubMed
698618	Palchevskiy, V.; Finkel, S.E.	A role for single-stranded exonucleases in the use of DNA as a nutrient	J. Bacteriol.	191	3712-3716	2009	Escherichia coli K-12	PubMed
700327	Serment-Guerrero, J.; Brena-Valle, M.; Espinosa-Aguirre, J.J.	In vivo role of Escherichia coli single-strand exonucleases in SOS induction by gamma radiation	Mutagenesis	23	317-323	2008	Escherichia coli	PubMed
700329	Kumar, G.A.; Woodhall, M.R.; Hood, D.W.; Moxon, E.R.; Bayliss, C.D.	RecJ, ExoI and RecG are required for genome maintenance but not for generation of genetic diversity by repeat-mediated phase variation in Haemophilus influenzae	Mutat. Res.	640	46-53	2008	Haemophilus influenzae	PubMed
700496	Larrea, A.A.; Pedroso, I.M.; Malhotra, A.; Myers, R.S.	Identification of two conserved aspartic acid residues required for DNA digestion by a novel thermophilic Exonuclease VII in Thermotoga maritima	Nucleic Acids Res.	36	5992-6003	2008	Escherichia coli, Thermotoga maritima	PubMed
707510	Noothi, S.K.; Minda, R.; Rao, B.J.	MutS and UvrD proteins stimulate exonuclease action: insights into exonuclease-mediated strand repair	Biochemistry	48	7787-7793	2009	Escherichia coli	PubMed
715119	Rudolph, C.J.; Mahdi, A.A.; Upton, A.L.; Lloyd, R.G.	RecG protein and single-strand DNA exonucleases avoid cell lethality associated with PriA helicase activity in Escherichia coli	Genetics	186	473-492	2010	Escherichia coli	PubMed

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