Information on EC 3.1.11.6 - exodeoxyribonuclease VII:

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The expected taxonomic range for this enzyme is: Bacteria


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EC NUMBERCOMMENTARY
3.1.11.6-

RECOMMENDED NAMEGeneOntology No.
exodeoxyribonuclease VIIGO:0009318

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates
show the reaction diagram
----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
hydrolysis of phosphoric ester----

PATHWAYKEGG LinkMetaCyc Link
No entries in this field

SYSTEMATIC NAMEIUBMB Comments
No entries in this field

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
E. coli exonuclease VII----
endodeoxyribonuclease VII----
Escherichia coli exonuclease VII----
exonuclease VII----
exonuclease VIIEscherichia coli--665060, 680517, 700496, 707510, 715119
exonuclease VIIEscherichia coli K-12, MG1655---
exonuclease VIIThermotoga maritima--700496
ExoVIIEscherichia coli--680517, 682560, 700327, 715119
ExoVIIEscherichia coli AB1157, Escherichia coli BW25113---
ExoVIIEscherichia coli K-12--698618
ExoVIIEscherichia coli K-12 ZK126, Escherichia coli K-12, MG1655---
ExoVIIHaemophilus influenzae--700329
ExoVIIHaemophilus influenzae RM118---
nuclease, exodeoxyribo-, VII----
RecBCDEscherichia coli--665060
XseAThermotoga maritima--700496
XseBThermotoga maritima--700496

CAS REGISTRY NUMBERCOMMENTARY
52933-20-7-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Escherichia coli-134040, 134045, 134046, 652155, 653673, 680517, 700496, 707510, 715119--Manually annotated by BRENDA team
Escherichia coligene recD665060--Manually annotated by BRENDA team
Escherichia colinumerous mutants; xseA134036--Manually annotated by BRENDA team
Escherichia colistrain AB1157682560--Manually annotated by BRENDA team
Escherichia colistrain BW25113700327--Manually annotated by BRENDA team
Escherichia colixseA134035, 134037, 134038, 134039, 134041, 134042, 134043, 134048--Manually annotated by BRENDA team
Escherichia colixseA134047P04994UniprotManually annotated by BRENDA team
Escherichia colixseB134044--Manually annotated by BRENDA team
Escherichia coli AB1157strain AB1157682560--Manually annotated by BRENDA team
Escherichia coli BW25113strain BW25113700327--Manually annotated by BRENDA team
Escherichia coli K-12strain ZK126698618--Manually annotated by BRENDA team
Escherichia coli K-12 ZK126strain ZK126698618--Manually annotated by BRENDA team
Escherichia coli K-12, MG1655-715119--Manually annotated by BRENDA team
Escherichia coli xseAxseA134035, 134036, 134037, 134038, 134039, 134041, 134042, 134043, 134048--Manually annotated by BRENDA team
Escherichia coli xseAxseA134047P04994UniprotManually annotated by BRENDA team
Escherichia coli xseBxseB134044--Manually annotated by BRENDA team
Haemophilus influenzaestrain RM118700329--Manually annotated by BRENDA team
Haemophilus influenzae RM118strain RM118700329--Manually annotated by BRENDA team
Thermotoga maritima-700496--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
malfunctionEscherichia coli-ExoI- ExoVII- SbcCD- cells are sensitive to mitomycin C and 2-aminopurine. Cells lacking all three nucleases, exonuclease I, exonuclease VII, or SbcCD, are inviable without RecG. Removing RNase HI mimics the effect of removing RecG in that DrnhA cells are inviable if ExoI, ExoVII, and SbcCD are eliminated715119
malfunctionEscherichia coli K-12, MG1655-ExoI- ExoVII- SbcCD- cells are sensitive to mitomycin C and 2-aminopurine. Cells lacking all three nucleases, exonuclease I, exonuclease VII, or SbcCD, are inviable without RecG. Removing RNase HI mimics the effect of removing RecG in that DrnhA cells are inviable if ExoI, ExoVII, and SbcCD are eliminated-
physiological functionEscherichia coli-mismatch repair proteins MutS and UvrD stimulate single-stranded DNA-specific exonuclease VII action on double-stranded DNA substrates. MutH and MutL proteins show no stimulation of exonuclease VII action. UvrD-mediated stimulation of exonuclease VII action on double-stranded DNA is not accompanied by strand separation. UvrD-mediated stimulation of exonuclease VII action on double-stranded DNA is accentuated by MutS and SSB proteins but not by MutL707510
physiological functionEscherichia coli-to stay alive, cells must have either RecG or a 3'-single-stranded DNA exonuclease, which can be exonuclease I, exonuclease VII, or SbcCD. Cells lacking all three nucleases are inviable without RecG715119
physiological functionEscherichia coli K-12, MG1655-to stay alive, cells must have either RecG or a 3'-single-stranded DNA exonuclease, which can be exonuclease I, exonuclease VII, or SbcCD. Cells lacking all three nucleases are inviable without RecG-

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
duplex DNA containing single-stranded termini + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134037-134037?
polydeoxyribonucleotides + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134035-134035?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134039-134039?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134040-134040?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134041-134041?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134042-134042?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134043-134043?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134044-134044?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134045-134045?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134046-134046?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coliP04994-134047-134047?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134048-134048?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--680517, 682560, 707510--?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134035oligonucleotides134035?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134036oligonucleotides134036?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134037oligonucleotides134037?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134038oligonucleotides134038?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli-no activity on circular DNA134037exclusively oligonucleotides134037?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli-no activity on circular DNA134038exclusively oligonucleotides, no mononucleotide production observed134038?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli-no endonuclease activity, no activity homopolymers134035limit products: oligonucleotides: dimers to dodecamers134035?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli-processive degradation134036-134036?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli-processive degradation134035limit products: oligonucleotides: dimers to dodecamers134035?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli-processive degradation134037exclusively oligonucleotides134037?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli-processive degradation134038exclusively oligonucleotides, no mononucleotide production observed134038?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli-no activity on DNA-RNA hybrid molecules134037exclusively oligonucleotides134037?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli-no activity on DNA-RNA hybrid molecules134038exclusively oligonucleotides, no mononucleotide production observed134038?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli-single stranded DNA of at least 10-20 nucleotides are required for maximal activity134038exclusively oligonucleotides, no mononucleotide production observed134038?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli-heat-denatured DNA, no activity on RNA134037exclusively oligonucleotides134037?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli-no activity on RNA134038exclusively oligonucleotides, no mononucleotide production observed134038?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseB--134044-134044?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA--134035oligonucleotides134035?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA-no endonuclease activity, no activity homopolymers, processive degradation134035limit products: oligonucleotides: dimers to dodecamers134035?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA--134036oligonucleotides134036?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA-processive degradation134036-134036?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA--134037oligonucleotides134037?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA-no activity on circular DNA, processive degradation, no activity on DNA-RNA hybrid molecules, heat-denatured DNA134037exclusively oligonucleotides134037?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA--134038oligonucleotides134038?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA-no activity on circular DNA134038exclusively oligonucleotides, no mononucleotide production observed134038?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA-processive degradation134038exclusively oligonucleotides, no mononucleotide production observed134038?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA--134039-134039?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA--134041-134041?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA--134042-134042?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA--134043-134043?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA--134048-134048?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli AB1157--682560--?
single-stranded DNA + H2O?
show the reaction diagram
Escherichia coli K-12--698618--?
single-stranded DNA + H2O?
show the reaction diagram
Thermotoga maritima-ExoVII activity is highly processive. It binds ssDNA ends and cleaves its substrate endonucleolytically, releasing oligonucleotide products of various sizes700496--?
single-stranded DNA + H2O?
show the reaction diagram
Escherichia coli-releases oligonucleotide products700496--?
single-stranded DNA + H2O?
show the reaction diagram
Escherichia coli K-12 ZK126--698618--?
thymine dimers + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coliP04994possible role in methyl-directed mismatch repair system134047-134047?
thymine dimers + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli-possible role in methyl-directed mismatch repair system134048-134048?
thymine dimers + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli-possible role in repair of ultraviolet damage134035-134035?
thymine dimers + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli-possible role in repair of ultraviolet damage134036-134036?
thymine dimers + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli-possible role in repair of ultraviolet damage134037-134037?
thymine dimers + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli-possible role in repair of ultraviolet damage134038-134038?
thymine dimers + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli-possible role in repair of ultraviolet damage134039-134039?
thymine dimers + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli-possible role in repair of ultraviolet damage134041-134041?
thymine dimers + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA-possible role in repair of ultraviolet damage134035-134035?
thymine dimers + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA-possible role in repair of ultraviolet damage134036-134036?
thymine dimers + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA-possible role in repair of ultraviolet damage134039-134039?
thymine dimers + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA-possible role in repair of ultraviolet damage134041-134041?
thymine dimers + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA-possible role in methyl-directed mismatch repair system134048-134048?
duplex DNA containing single-stranded termini + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134038-134038?
additional information?-Escherichia coli-involved in methyl-directed mismatch repair653673---
additional information?-Escherichia coli-RecBCD subunit RecD is required for initiation of homologous recombination in Escherichia coli and essential for phage lambda recombination, in contrast to exonuclease RecJ, overview665060---
additional information?-Haemophilus influenzae-ExoVII is refractory to mutation, inactivation appears to be lethal. ExoVII appears to be essential for growth of Haemophilus influenzae, possibly, given its ability to process DNA flaps, indicating a requirement for the activities of this protein in replicative DNA synthesis700329---
additional information?-Escherichia coli K-12-mutants lacking ExoVII are viable during all phases of the bacterial life cycle, but cannot compete with wild-type cells during long-term stationary phase incubation. Mutants lacking ExoVII are defective in DNA catabolism698618---
additional information?-Thermotoga maritima-active site motif with two conserved aspartate residues in the large (XseA/TM1768) subunit. Residues Asp235 and Asp240 are essential for the nuclease activity, no significant activity on dsDNA. Active site motif with two conserved aspartate residues in the large (XseA/TM1768) subunit. Residues Asp235 and Asp240 are essential for the nuclease activity700496---
additional information?-Escherichia coli-exonuclease VII can digest ssDNA from 3' ends715119---
additional information?-Escherichia coli K-12 ZK126-mutants lacking ExoVII are viable during all phases of the bacterial life cycle, but cannot compete with wild-type cells during long-term stationary phase incubation. Mutants lacking ExoVII are defective in DNA catabolism698618---
additional information?-Haemophilus influenzae RM118-ExoVII is refractory to mutation, inactivation appears to be lethal. ExoVII appears to be essential for growth of Haemophilus influenzae, possibly, given its ability to process DNA flaps, indicating a requirement for the activities of this protein in replicative DNA synthesis700329---
additional information?-Escherichia coli K-12, MG1655-exonuclease VII can digest ssDNA from 3' ends715119---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134035oligonucleotides134035?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134036oligonucleotides134036?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134037oligonucleotides134037?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli--134038oligonucleotides134038?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA--134035oligonucleotides134035?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA--134036oligonucleotides134036?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA--134037oligonucleotides134037?
single-stranded DNA + H2Ohydrolyzed single-stranded DNA
show the reaction diagram
Escherichia coli xseA--134038oligonucleotides134038?
additional information?-Escherichia coli-involved in methyl-directed mismatch repair653673---
additional information?-Escherichia coli-RecBCD subunit RecD is required for initiation of homologous recombination in Escherichia coli and essential for phage lambda recombination, in contrast to exonuclease RecJ, overview665060---
additional information?-Haemophilus influenzae-ExoVII is refractory to mutation, inactivation appears to be lethal. ExoVII appears to be essential for growth of Haemophilus influenzae, possibly, given its ability to process DNA flaps, indicating a requirement for the activities of this protein in replicative DNA synthesis700329---
additional information?-Escherichia coli K-12-mutants lacking ExoVII are viable during all phases of the bacterial life cycle, but cannot compete with wild-type cells during long-term stationary phase incubation. Mutants lacking ExoVII are defective in DNA catabolism698618---
additional information?-Escherichia coli-exonuclease VII can digest ssDNA from 3' ends715119---
additional information?-Escherichia coli K-12 ZK126-mutants lacking ExoVII are viable during all phases of the bacterial life cycle, but cannot compete with wild-type cells during long-term stationary phase incubation. Mutants lacking ExoVII are defective in DNA catabolism698618---
additional information?-Haemophilus influenzae RM118-ExoVII is refractory to mutation, inactivation appears to be lethal. ExoVII appears to be essential for growth of Haemophilus influenzae, possibly, given its ability to process DNA flaps, indicating a requirement for the activities of this protein in replicative DNA synthesis700329---
additional information?-Escherichia coli K-12, MG1655-exonuclease VII can digest ssDNA from 3' ends715119---

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
KClThermotoga maritima-ExoVII activity is maximal at low salt concentrations (lower than 250 mM)700496
Mg2+Escherichia coli-25% activation134037
Mg2+Thermotoga maritima-robust nuclease activity at 80C is magnesium dependent700496
additional informationThermotoga maritima-manganese, nickel, cobalt and zinc have no effect700496

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
EDTAEscherichia coli-can funtion in absence of divalent cations134035 2D-image
EDTAEscherichia coli-can funtion in absence of divalent cations; slight inhibition134037 2D-image
N-ethylmaleimideEscherichia coli-47% inhibition in absence of mercaptoethanol134037 2D-image
phosphateThermotoga maritima-inhibits nuclease activity at 80C; strongly inhibits, almost complete inhibition by 50 mM700496 2D-image
sulfateThermotoga maritima-strongly inhibits, almost complete inhibition by 50 mM700496 2D-image
EDTAThermotoga maritima-; completely inhibits700496 2D-image
additional informationThermotoga maritima-is not active in standard Escherichia coli ExoVII buffer. Acetate has no effect700496-

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
phosphateEscherichia coli-5-10fold higher activity compared to Tris-HCL buffer134037 2D-image
phosphateEscherichia coli-stimulates700496 2D-image
sulfateEscherichia coli-stimulates700496 2D-image
2-mercaptoethanolEscherichia coli-required for 100% activity134037 2D-image
additional informationEscherichia coli-becomes activated upon DNA damage by single-stranded DNA regions, in order to confer better opportunities for cell survival700327-
additional informationEscherichia coli-acetate has no effect700496-

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
221-Thermotoga maritima--700496
267-Escherichia coli--700496

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
7.87.9Escherichia coli--134037
7.9-Thermotoga maritima--700496

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
37-Escherichia coli-assay134037
80-Thermotoga maritima-XseA/B homologs TM1768 and TM1769 show robust and maximal nuclease activity700496

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
No entries in this field

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
cytoplasmEscherichia coli K-12--5737698618Manually annotated by BRENDA team
cytoplasmEscherichia coli K-12 ZK126--5737-Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
1vp7, downloadSCOP (1vp7)CATH (1vp7)Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
51820-Escherichia coli-large subunit, amino acid sequence134046
88000-Escherichia coli-gel fltration134037

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
pentamerEscherichia coli-heterodimer, 1 * 54000, 4 * 105000, large subunit: xseA gene, small subunit: xseB gene, SDS-PAGE134042, 134044
pentamerEscherichia coli xseA, Escherichia coli xseB-heterodimer, 1 * 54000, 4 * 105000, large subunit: xseA gene, small subunit: xseB gene, SDS-PAGE-
additional informationThermotoga maritima-stoichiometry of small to large subunits regulates ExoVII activity700496

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
7.37.8Escherichia coli-50% loss of activity compared to pH 7.8134037
7.88.6Escherichia coli-50% loss of activity compared to pH 7.8134037

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
37-Escherichia coli-half-life: 90 min134037
45-Escherichia coli-half-life: 45 min134037
60-Escherichia coli-half-life: 7 min134037
80-Thermotoga maritima-XseA/B homologs TM1768 and TM1769 show robust nuclease activity700496

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
freezing and thawing causes loss of activityEscherichia coli-134036

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-20C, 20 mM Tris-HCl, pH 8, 10 mM mercaptoethanol, 0.1 mM EDTA, 0.2 M NaCl, 50% glycerol, 18 months, 10% loss of activityEscherichia coli-134036

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
1700foldEscherichia coli-134036, 134037
7500fold to 87% homogeneityEscherichia coli-134042
wild-type and mutant ExoVII (XseA/B homologs TM1768 and TM1769) purified by gel filtration and on nickel-NTA column; wild-type and mutant ExoVII (XseA/B homologs TM1768 and TM1769) purified on nickel-NTA columnThermotoga maritima-700496

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
large subunitEscherichia coli-134045
strain deficient in enzymic activity plus deficiency in RecJ, ExoI and ExoX exonuclease activities as well as a strain deficient in RecJ, ExoVII and ExoI exonucleases grow poorly in presence of 2-aminopurine. Exposure to 2-aminopurine results in filament formation. The quadruple mutant is cold-sensitive with a severe growth defect at 30CEscherichia coli-653673
strain deficient in enzymic activity plus deficiency in RecJ, ExoI and ExoX exonuclease activities displays a 7fold increase in mutation rate, the availabilty of any one of thr nucleases is enough to support full mismatch correctionEscherichia coli-652155
wild-type and mutant ExoVII (XseA/B homologs TM1768 and TM1769) coexpressed in Escherichia coli BL21(DE3) cellsThermotoga maritima-700496

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
D235A/D240AThermotoga maritima-is completely inactive, even in the presence of magnesium700496
additional informationEscherichia coli-mutants lacking the xseA gene134047, 134048
additional informationEscherichia coli-construction of recD mutants showing a RecBC enzyme, instead of a RecBCD enzyme, lacking the RecD subunit and exonuclease VII activity, the mutation affects phage lambda red gam recombination, but does not cause an altered phenotype, overview665060
additional informationEscherichia coli-recJ xseA sbcCD xonA2 quadruple mutants are cold sensitive depending on the xon allele. Absence of the two 3' exonucleases ExoI and ExoVII allows highly efficient conjugational recombination in recBCD+ cells, which is independent of SbcCD, recJ xseA double mutant has lower UV-survival than wild-type680517
additional informationEscherichia coli-mutant in all four exonucleases (ExoI, ExoVII, ExoX, and RecJ) exhibits pronounced 20fold elevation of cross-overs between 25 bp of homology relative to the wild-type682560
additional informationEscherichia coli-conbstruction of ExoVII-deficient cells and of cells lacking alll three exonuxleases, exonuclease I, exonuclease VII, or SbcCD, phenotypes, detailed overview715119
additional informationEscherichia coli AB1157-mutant in all four exonucleases (ExoI, ExoVII, ExoX, and RecJ) exhibits pronounced 20fold elevation of cross-overs between 25 bp of homology relative to the wild-type-
additional informationEscherichia coli K-12, MG1655-conbstruction of ExoVII-deficient cells and of cells lacking alll three exonuxleases, exonuclease I, exonuclease VII, or SbcCD, phenotypes, detailed overview-
additional informationEscherichia coli xseA-mutants lacking the xseA gene-

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
analysisEscherichia coli-degradation of adenovirus-associated DNA; excision of DNA segments from cloning vectors; of nucleic acid structure; of spliced RNA; removal of single-stranded DNA134036
analysisEscherichia coli-purification of DNA rings134040
analysisEscherichia coli-xseA-mutants for measurement of single-stranded apurinic-apyrimidinic endonuclease134043
analysisEscherichia coli xseA-degradation of adenovirus-associated DNA; excision of DNA segments from cloning vectors; of nucleic acid structure; of spliced RNA; removal of single-stranded DNA; xseA-mutants for measurement of single-stranded apurinic-apyrimidinic endonuclease-
analysisEscherichia coli-mapping of mRNA isoforms134046
additional informationEscherichia coli-ExoVII, encoded by xseA, has an antirecombination function680517
additional informationEscherichia coli-RecA-independent recombination is depressed by the redundant action of single-strand exonucleases. ExoVII inhibits crossing-over682560
additional informationEscherichia coli AB1157-RecA-independent recombination is depressed by the redundant action of single-strand exonucleases. ExoVII inhibits crossing-over-

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISM (UNIPROT ACCESSION NO.)LINK TO PUBMEDSOURCE
134035Chase, J.W.; Richardson, C.C.Exonuclease VII of Escherichia coliBasic Life Sci.5A225-2341975Escherichia coli, Escherichia coli xseA PubMed
134036Chase, J.W.; Vales, L.D.Exonuclease VII of E. coliGene Amplif. Anal.2147-1681981Escherichia coli, Escherichia coli xseA PubMed
134037Chase, J.W.; Richardson, C.C.Exonuclease VII of Escherichia coli. Purification and propertiesJ. Biol. Chem.2494545-45521974Escherichia coli, Escherichia coli xseA PubMed
134038Chase, J.W.; Richardson, C.C.Exonuclease VII of Escherichia coli. Mechanism of actionJ. Biol. Chem.2494553-45611974Escherichia coli, Escherichia coli xseA PubMed
134039Chase, J.W.; Masker, W.E.Deoxyribonucleic acid repair in Escherichia coli mutants deficient in the 5'-3' exonuclease activity of deoxyribonucleic acid polymerase I and exonuclease VIIJ. Bacteriol.130667-6751977Escherichia coli, Escherichia coli xseA PubMed
134040Hutton, J.R.; Thomas, C.A.The assay and isolation of DNA rings using an ATP-dependent endonucleaseBiochemistry141432-14361975Escherichia coli PubMed
134041Chase, J.W.; Masker, W.E.; Murphy, J.B.Pyrimidine dimer excision in Escherichia coli strains deficient in exonucleases V and VII and in the 5' leads to 3' exonuclease of DNA polymerase IJ. Bacteriol.137234-2421979Escherichia coli, Escherichia coli xseA PubMed
134042Vales, L.D.; Rabin, B.A.; Chase, J.W.Subunit structure of Escherichia coli exonuclease VIIJ. Biol. Chem.2578799-88051982Escherichia coli, Escherichia coli xseA PubMed
134043Schultz, R.A.; Friedberg, E.C.; Moses, R.E.; Rupp, W.D.; Sancar, A.; Sharma, S.Use of an Escherichia coli mutant strain permits measurement of single-stranded apurinic-apyrimidinic endonuclease in crude extracts: studies with untransformed cells and cells transformed with plasmids containing the uvrC geneJ. Bacteriol.1541459-14611983Escherichia coli, Escherichia coli xseA PubMed
134044Vales, L.D.; Rabin, B.A.; Chase, J.W.Isolation and preliminary characterization of Escherichia coli mutants deficient in exonuclease VIIJ. Bacteriol.1551116-11221983Escherichia coli, Escherichia coli xseB PubMed
134045Chase, J.W.; Rabin, B.A.; Murphy, J.B.; Stone, K.L.; Williams, K.R.Escherichia coli exonuclease VII. Cloning and sequencing of the gene encoding the large subunit (xseA)J. Biol. Chem.26114929-149351986Escherichia coli PubMed
134046Chassagne, C.; Schwartz, K.Mapping of mRNA isoforms with an oligonucleotide probe: exonuclease VII compared with endonucleasesNucleic Acids Res.2032561992Escherichia coli PubMed
134047Harris, R.S.; Ross, K.J.; Lombardo, M.J.; Rosenberg, S.M.Mismatch repair in Escherichia coli cells lacking single-strand exonucleases ExoI, ExoVII, and RecJJ. Bacteriol.180989-9931998Escherichia coli, Escherichia coli (P04994), Escherichia coli xseA (P04994) PubMed
134048Viswanathan, M.; Lovett, S.T.Single-strand DNA-specific exonucleases in Escherichia coli. Roles in repair and mutation avoidanceGenetics1497-161998Escherichia coli, Escherichia coli xseA PubMed
652155Viswanathan, M.; Burdett, V.; Baitinger, C.; Modrich, P.; Lovett, S.T.Redundant exonuclease involvement in Escherichia coli methyl-directed mismatch repairJ. Biol. Chem.27631053-310582001Escherichia coli PubMed
653673Burdett, V.; Baitinger, C.; Viswanathan, M.; Lovett, S.T.; Modrich, P.In vivo requirement for RecJ, ExoVII, ExoI, and ExoX in methyl-directed mismatch repairProc. Natl. Acad. Sci. USA986765-67702001Escherichia coli PubMed
665060Dermic, D.; Zahradka, D.; Petranovic, M.Exonuclease requirements for recombination of lambda phage in recD mutants of Escherichia coliGenetics1732399-24022006Escherichia coli PubMed
680517Thoms, B.; Borchers, I.; Wackernagel, W.Effects of single-strand DNases ExoI, RecJ, ExoVII, and SbcCD on homologous recombination of recBCD+ strains of Escherichia coli and roles of SbcB15 and XonA2 ExoI mutant enzymesJ. Bacteriol.190179-1922008Escherichia coli PubMed
682560Dutra, B.E.; Sutera, V.A.; Lovett, S.T.RecA-independent recombination is efficient but limited by exonucleasesProc. Natl. Acad. Sci. USA104216-2212007Escherichia coli, Escherichia coli AB1157 PubMed
698618Palchevskiy, V.; Finkel, S.E.A role for single-stranded exonucleases in the use of DNA as a nutrientJ. Bacteriol.1913712-37162009Escherichia coli K-12, Escherichia coli K-12 ZK126 PubMed
700327Serment-Guerrero, J.; Brena-Valle, M.; Espinosa-Aguirre, J.J.In vivo role of Escherichia coli single-strand exonucleases in SOS induction by gamma radiationMutagenesis23317-3232008Escherichia coli, Escherichia coli BW25113 PubMed
700329Kumar, G.A.; Woodhall, M.R.; Hood, D.W.; Moxon, E.R.; Bayliss, C.D.RecJ, ExoI and RecG are required for genome maintenance but not for generation of genetic diversity by repeat-mediated phase variation in Haemophilus influenzaeMutat. Res.64046-532008Haemophilus influenzae, Haemophilus influenzae RM118 PubMed
700496Larrea, A.A.; Pedroso, I.M.; Malhotra, A.; Myers, R.S.Identification of two conserved aspartic acid residues required for DNA digestion by a novel thermophilic Exonuclease VII in Thermotoga maritimaNucleic Acids Res.365992-60032008Escherichia coli, Thermotoga maritima PubMed
707510Noothi, S.K.; Minda, R.; Rao, B.J.MutS and UvrD proteins stimulate exonuclease action: insights into exonuclease-mediated strand repairBiochemistry487787-77932009Escherichia coli PubMed
715119Rudolph, C.J.; Mahdi, A.A.; Upton, A.L.; Lloyd, R.G.RecG protein and single-strand DNA exonucleases avoid cell lethality associated with PriA helicase activity in Escherichia coliGenetics186473-4922010Escherichia coli, Escherichia coli K-12, MG1655 PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 3.1.11.6)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)