Information on EC 2.7.7.28 - nucleoside-triphosphate-aldose-1-phosphate nucleotidyltransferase:
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The lowest common taxonomy group for this enzyme is: Eutheria

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EC NUMBERCOMMENTARY
2.7.7.28-

RECOMMENDED NAMEGeneOntology No.
nucleoside-triphosphate-aldose-1-phosphate nucleotidyltransferaseGO:0047339

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
nucleoside triphosphate + alpha-D-aldose 1-phosphate = diphosphate + NDP-hexose
show the reaction diagram		----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
nucleotidyl group transfer----

PATHWAYKEGG LinkMetaCyc Link
No entries in this field

SYSTEMATIC NAMEIUBMB Comments
NTP:alpha-D-aldose-1-phosphate nucleotidyltransferaseIn decreasing order of activity, guanosine, inosine and adenosine diphosphate hexoses are substrates in the reverse reaction, with either glucose or mannose as the sugar.

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
EC 2.7.7.29--formerly-
GDP hexose pyrophosphorylase----
GDPhexose pyrophosphorylase----
GTP:alpha-D-hexose-1-phosphate guanylyltransferase----
guanosine diphosphohexose pyrophosphorylase----
guanylyltransferase, hexose 1-phosphate----
hexose 1-phosphate guanylyltransferase----
hexose 1-phosphate nucleotidyltransferase----
hexose nucleotidylating enzyme----
hexose-1-phosphate guanylyltransferase----
NDP hexose pyrophosphorylase----
NTP:hexose-1-phosphate nucleotidyltransferase----
nucleoside diphosphohexose pyrophosphorylase----
nucleoside-triphosphate-hexose-1-phosphate nucleotidyltransferase----
nucleotidyltransferase, hexose 1-phosphate----

CAS REGISTRY NUMBERCOMMENTARY
37278-26-5-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Bos taurus-645219, 645220--Manually annotated by BRENDA team
Rattus norvegicus-645219--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
ADPmannose + diphosphateATP + D-mannose 1-phosphate
show the reaction diagram		Bos taurus-the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose645220---
GDPglucose + diphosphateGTP + D-glucose 1-phosphate
show the reaction diagram		Bos taurus-best substrate645219-645219r
GDPglucose + diphosphateGTP + D-glucose 1-phosphate
show the reaction diagram		Bos taurus-the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose645220-645220r
GDPmannose + diphosphateGTP + D-mannose 1-phosphate
show the reaction diagram		Bos taurus--645219-645219r
GDPmannose + diphosphateGTP + D-mannose 1-phosphate
show the reaction diagram		Bos taurus-the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose645220---
GDPmannose + diphosphateGTP + D-mannose 1-phosphate
show the reaction diagram		Rattus norvegicus-reaction at about half the rate of GDPglucose645219---
IDPglucose + diphosphateITP + D-glucose 1-phosphate
show the reaction diagram		Bos taurus-the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose645220---
IDPglucose + diphosphateITP + D-glucose 1-phosphate
show the reaction diagram		Bos taurus-reaction at 21% the rate of GDPglucose645219-645219r
IDPmannose + diphosphateITP + D-mannose 1-phosphate
show the reaction diagram		Bos taurus--645219-645219r
IDPmannose + diphosphateITP + D-mannose 1-phosphate
show the reaction diagram		Bos taurus-the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose645220--r
IDPmannose + diphosphateITP + D-mannose 1-phosphate
show the reaction diagram		Rattus norvegicus-reaction at 7% the rate of GDPglucose645219---
UDPglucose + diphosphateUTP + D-glucose 1-phosphate
show the reaction diagram		Bos taurus-the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose645220---
UDPmannose + diphosphateUTP + D-mannose 1-phosphate
show the reaction diagram		Bos taurus-the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose645220---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
ADPmannose + diphosphateATP + D-mannose 1-phosphate
show the reaction diagram		Bos taurus-the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose645220--
GDPglucose + diphosphateGTP + D-glucose 1-phosphate
show the reaction diagram		Bos taurus-best substrate645219-645219
GDPglucose + diphosphateGTP + D-glucose 1-phosphate
show the reaction diagram		Bos taurus-the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose645220-645220
GDPmannose + diphosphateGTP + D-mannose 1-phosphate
show the reaction diagram		Bos taurus--645219-645219
GDPmannose + diphosphateGTP + D-mannose 1-phosphate
show the reaction diagram		Bos taurus-the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose645220--
GDPmannose + diphosphateGTP + D-mannose 1-phosphate
show the reaction diagram		Rattus norvegicus-reaction at about half the rate of GDPglucose645219--
IDPglucose + diphosphateITP + D-glucose 1-phosphate
show the reaction diagram		Bos taurus-the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose645220--
IDPglucose + diphosphateITP + D-glucose 1-phosphate
show the reaction diagram		Bos taurus-reaction at 21% the rate of GDPglucose645219-645219
IDPmannose + diphosphateITP + D-mannose 1-phosphate
show the reaction diagram		Bos taurus--645219-645219
IDPmannose + diphosphateITP + D-mannose 1-phosphate
show the reaction diagram		Bos taurus-the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose645220--
IDPmannose + diphosphateITP + D-mannose 1-phosphate
show the reaction diagram		Rattus norvegicus-reaction at 7% the rate of GDPglucose645219--
UDPglucose + diphosphateUTP + D-glucose 1-phosphate
show the reaction diagram		Bos taurus-the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose645220--
UDPmannose + diphosphateUTP + D-mannose 1-phosphate
show the reaction diagram		Bos taurus-the enzyme catalyzes the pyrophosphorolysis of the following nucleoside diphosphate hexoses in decreasing order of activity: GDPglucose, GDPmannose, IDPmannose, IDPglucose, ADPmannose, UDPglucose and UDPmannose645220--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
Co2+Bos taurus-metal requirement can be met by Co2+645219
Mg2+Bos taurus-requirement, maximal activity at about 5 mM, maximal activity at about twice the diphosphate-concentration645219
Mg2+Bos taurus-required645220
Mn2+Bos taurus-metal requirement can be partially met by Mn2+645220

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
D-mannose 1-phosphateBos taurus-strong645219 2D-image
D-mannose 1-phosphateBos taurus--645220 2D-image
GDPmannoseBos taurus-strong645219 2D-image
GDPmannoseBos taurus--645220 2D-image
Zn2+Bos taurus-inhibits when added with Mg2+, Mn2+ or Co2+645220 2D-image

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
2.6-D-glucose 1-phosphateBos taurus-pH 7.8, 25°C645219 2D-image
2.6-D-glucose 1-phosphateBos taurus--645220 2D-image
0.082-diphosphateBos taurus-pH 7.8, 25°C645219 2D-image
0.082-diphosphateBos taurus--645220 2D-image
0.62-GDPglucoseBos taurus-pH 7.8, 25°C645219 2D-image
0.0001-GDPmannoseBos taurus-pH 7.8, 25°C645219 2D-image
0.0001-GDPmannoseBos taurus--645220 2D-image
2.6-GTPBos taurus-pH 7.8, 25°C645219 2D-image
2.6-GTPBos taurus--645220 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.047-D-mannose 1-phosphateBos taurus-pH 7.8, 25°C645219 2D-image
0.047-D-mannose 1-phosphateBos taurus--645220 2D-image
0.00017-GDP-mannoseBos taurus-pH 7.8, 25°C645219 2D-image
0.00017-GDP-mannoseBos taurus--645220 2D-image

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
0.38-Bos taurus--645219, 645220

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
7.58Bos taurus--645219, 645220

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
6.59Bos taurus, Rattus norvegicus-pH 6.5: about 40% of maximal activity, pH 9.0: about half-maximal activity645219

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
brainRattus norvegicus--645219Manually annotated by BRENDA team
kidneyRattus norvegicus--645219Manually annotated by BRENDA team
liverBos taurus--645219, 645220Manually annotated by BRENDA team
liverRattus norvegicus--645219Manually annotated by BRENDA team
mammary glandRattus norvegicus--645219Manually annotated by BRENDA team
muscleRattus norvegicus--645219Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
cytoplasmBos taurus, Rattus norvegicus--5737645219Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
66.5Bos taurus-t1/2: 60 days at 4°C645219

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
50-Bos taurus-15% loss of activity after 5 min, 33% loss of activity after 10 min645219
55-Bos taurus-1 min, 57% loss of activity645219

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
4°C, at pH 6-6.5, t1/2: 60 daysBos taurus-645219

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Bos taurus-645219, 645220

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
645219Verachtert, H.; Rodriguez, P.; Bass, S.T.; Hansen, R.G.Purification and properties of guanosine diphosphate hexose pyrophosphorylase from mammalian tissuesJ. Biol. Chem.2412007-20131966Bos taurus, Rattus norvegicus PubMed
645220Hansen, R.G.; Verachtert, H.; Rodriguez, P.; Bass, S.T.GDP-hexose pyrophosphorylase from liverMethods Enzymol.8269-2711966Bos taurus-

LINKS TO OTHER DATABASES (specific for EC-Number 2.7.7.28)
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NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
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Protein Mutant Database
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