Information on EC 1.4.3.10 - putrescine oxidase:
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EC NUMBERCOMMENTARY
1.4.3.10-

RECOMMENDED NAMEGeneOntology No.
putrescine oxidaseGO:0050232

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
putrescine + O2 + H2O = 4-aminobutanal + NH3 + H2O2
show the reaction diagram		----
putrescine + O2 + H2O = 4-aminobutanal + NH3 + H2O2
show the reaction diagram		mechanismKocuria rosea-395447
putrescine + O2 + H2O = 4-aminobutanal + NH3 + H2O2
show the reaction diagram		-Kocuria rosea-654187

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
oxidation----
oxidative deamination----
redox reaction----
reduction----

PATHWAYKEGG LinkMetaCyc Link
putrescine degradation IV-PWY-2

SYSTEMATIC NAMEIUBMB Comments
putrescine:oxygen oxidoreductase (deaminating)A flavoprotein (FAD). 4-Aminobutanal condenses non-enzymically to 1-pyrroline.

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
oxidase, putrescine----
PO----
PuOKocuria rosea--654187
PuORhodococcus erythropolis--684634, 687763

CAS REGISTRY NUMBERCOMMENTARY
9076-87-3-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Bos taurus-395442, 395449--Manually annotated by BRENDA team
Candida albicans-395444--Manually annotated by BRENDA team
Candida parapsilosis-395444--Manually annotated by BRENDA team
Candida tropicalis-395444--Manually annotated by BRENDA team
Emericella nidulans-395441--Manually annotated by BRENDA team
Kocuria rosea-395436, 395437, 395438, 395443, 395446, 395447, 395448, 395450, 654187--Manually annotated by BRENDA team
Meyerozyma guilliermondii-395444--Manually annotated by BRENDA team
Micrococcus luteus-395440--Manually annotated by BRENDA team
Pichia kudriavzevii-395444--Manually annotated by BRENDA team
Pisum sativum-395445--Manually annotated by BRENDA team
Pseudomonas putida-288077--Manually annotated by BRENDA team
Rhodococcus erythropolisfragment; strain NCIMB 11540684634B0F9F6UniProtManually annotated by BRENDA team
Rhodococcus erythropolisstrain NCIMB 11540687763--Manually annotated by BRENDA team
Rhodococcus erythropolis NCIMB 11540fragment; strain NCIMB 11540684634B0F9F6UniProtManually annotated by BRENDA team
Rhodococcus erythropolis NCIMB 11540strain NCIMB 11540687763--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
1,3-diaminopropane + O2 + H2O3-aminopropanal + NH3 + H2O2
show the reaction diagram		Bos taurus--395442--?
1,6-diaminohexane + O2 + H2O?
show the reaction diagram		Bos taurus--395442--?
1,6-diaminohexane + O2 + H2O?
show the reaction diagram		Kocuria rosea-1% of the activity with putrescine395447--?
1,6-diaminohexane + O2 + H2O6-aminohexanal + NH3 + H2O2
show the reaction diagram		Rhodococcus erythropolisB0F9F61.4% activity compared to putrescine684634--?
1,7-diaminoheptane + O2 + H2O?
show the reaction diagram		Bos taurus--395442--?
2-hydroxyputrescine + O2 + H2O?
show the reaction diagram		Rhodococcus erythropolisB0F9F628% activity compared to putrescine684634--?
4-amino-1-butanol + O2 + H2O4-hydroxybutanal + NH3 + H2O2
show the reaction diagram		Rhodococcus erythropolisB0F9F60.6% activity compared to putrescine684634--?
benzylamine + O2 + H2Obenzaldehyde + NH3 + H2O2
show the reaction diagram		Bos taurus--395442--?
cadaverine + O2 + H2O5-aminopentanal + NH3 + H2O2
show the reaction diagram		Bos taurus--395442--?
cadaverine + O2 + H2O5-aminopentanal + NH3 + H2O2
show the reaction diagram		Kocuria rosea--395437--?
cadaverine + O2 + H2O5-aminopentanal + NH3 + H2O2
show the reaction diagram		Micrococcus luteus--395440--?
cadaverine + O2 + H2O5-aminopentanal + NH3 + H2O2
show the reaction diagram		Kocuria rosea-7% of the activity with putrescine395447--?
cadaverine + O2 + H2O5-aminopentanal + NH3 + H2O2
show the reaction diagram		Pseudomonas putida-at 11% of the activity with putrescine288077--?
cadaverine + O2 + H2O5-aminopentanal + NH3 + H2O2
show the reaction diagram		Meyerozyma guilliermondii-47% of the activity with putrescine395444--?
cadaverine + O2 + H2O5-aminopentanal + NH3 + H2O2
show the reaction diagram		Rhodococcus erythropolisB0F9F614% activity compared to putrescine684634--?
ethylamine + O2 + H2O?
show the reaction diagram		Bos taurus--395442--?
histamine + O2 + H2O?
show the reaction diagram		Bos taurus--395442--?
histamine + O2 + H2O?
show the reaction diagram		Meyerozyma guilliermondii-no activity395444---
N-acetylputrescine + O2 + H2ON4-acetylaminobutanal + NH3 + H2O2
show the reaction diagram		Bos taurus--395442--?
N1-acetylspermidine + O2 + H2O?
show the reaction diagram		Bos taurus--395442--?
N8-acetylspermidine + O2 + H2O?
show the reaction diagram		Bos taurus--395442--?
putrescine + O2 + H2O4-aminobutanal + NH3 + H2O2
show the reaction diagram		Bos taurus--395442, 395449--?
putrescine + O2 + H2O4-aminobutanal + NH3 + H2O2
show the reaction diagram		Emericella nidulans--395441--?
putrescine + O2 + H2O4-aminobutanal + NH3 + H2O2
show the reaction diagram		Pisum sativum--395445--?
putrescine + O2 + H2O4-aminobutanal + NH3 + H2O2
show the reaction diagram		Kocuria rosea--395436, 395437, 395438, 395443, 395446, 395447, 395448, 395450--?
putrescine + O2 + H2O4-aminobutanal + NH3 + H2O2
show the reaction diagram		Pseudomonas putida--288077--?
putrescine + O2 + H2O4-aminobutanal + NH3 + H2O2
show the reaction diagram		Pichia kudriavzevii--395444--?
putrescine + O2 + H2O4-aminobutanal + NH3 + H2O2
show the reaction diagram		Micrococcus luteus--395440--?
putrescine + O2 + H2O4-aminobutanal + NH3 + H2O2
show the reaction diagram		Meyerozyma guilliermondii, Candida albicans, Candida tropicalis--395444--?
putrescine + O2 + H2O4-aminobutanal + NH3 + H2O2
show the reaction diagram		Rhodococcus erythropolis--687763--?
putrescine + O2 + H2O4-aminobutanal + NH3 + H2O2
show the reaction diagram		Candida parapsilosis--395444--?
putrescine + O2 + H2O4-aminobutanal + NH3 + H2O2
show the reaction diagram		Rhodococcus erythropolisB0F9F6100% activity684634--?
putrescine + O2 + H2O4-aminobutanal + NH3 + H2O2
show the reaction diagram		Kocuria rosea-primary electron acceptor is FAD, reversible reaction is FADH2 + O2 = FAD + H2O2654187--?
spermidine + O2 + H2O4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
show the reaction diagram		Kocuria rosea--395448--?
spermidine + O2 + H2O4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
show the reaction diagram		Kocuria rosea--395437no liberation of ammonia395437?
spermidine + O2 + H2O4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
show the reaction diagram		Kocuria rosea--395450no liberation of ammonia395450?
spermidine + O2 + H2O4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
show the reaction diagram		Bos taurus-no activity395449---
spermidine + O2 + H2O4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
show the reaction diagram		Bos taurus-no activity at spermidine concentrations of 0.0175 mM, activity at 1 mM395442--?
spermidine + O2 + H2O4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
show the reaction diagram		Meyerozyma guilliermondii-51% of the activity with putrescine395444--?
spermidine + O2 + H2O4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
show the reaction diagram		Kocuria rosea-10% of the activity with putrescine395447--?
spermidine + O2 + H2O4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
show the reaction diagram		Pseudomonas putida-at 9% of the activity with putrescine288077--?
spermidine + O2 + H2O4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
show the reaction diagram		Rhodococcus erythropolisB0F9F65.3% activity compared to putrescine684634--?
spermine + O2 + H2ON,N'-bis(3-aminopropyl)-4-aminobutanal + NH3 + H2O2
show the reaction diagram		Bos taurus--395442--?
spermine + O2 + H2ON,N'-bis(3-aminopropyl)-4-aminobutanal + NH3 + H2O2
show the reaction diagram		Meyerozyma guilliermondii-1.7% of the activity with putrescine395444--?
spermine + O2 + H2ON,N'-bis(3-aminopropyl)-4-aminobutanal + NH3 + H2O2
show the reaction diagram		Rhodococcus erythropolisB0F9F60.7% activity compared to putrescine684634--?
L-ornithine + O2 + H2O?
show the reaction diagram		Rhodococcus erythropolisB0F9F60.08% activity compared to putrescine684634--?
additional information?-Kocuria rosea-the enzyme modified with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide shows activity towards monoamines such as n-butylamine, n-hexylamine and n-octylamine, which are not substrates of the native enzyme395448---
additional information?-Kocuria rosea-the enzyme is a weak 2-mercaptoethanol oxidase395438---
additional information?-Rhodococcus erythropolisB0F9F6no activity with aromatic amines and amino alcohols684634---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
No entries in this field

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
FADKocuria rosea-contains 1 mol of FAD per mol of enzyme395437 2D-image
FADKocuria rosea-contains 1 FAD per 82000 Da395438 2D-image
FADKocuria rosea-contains FAD395443, 395448, 395450 2D-image
FADKocuria rosea-bound to the enzyme654187 2D-image
FADRhodococcus erythropolisB0F9F6contains non-covalently bound FAD684634 2D-image
FADRhodococcus erythropolis-the enzyme is isolated as a mixture of dimers containing two molecules of FAD, two molecules ADP, or one FAD and one ADP molecule, the low FAD:protein ratio is the result of tight binding of ADP thereby competing with FAD binding687763 2D-image

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
additional informationMeyerozyma guilliermondii-no activation by divalent cations395444

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
(2-aminoethyl)-trimethylammoniumRhodococcus erythropolisB0F9F6competitive inhibitor684634 2D-image
1,1,4,4-tetramethyl-1,4-diaminobutaneKocuria rosea--395447 2D-image
1,10-DiaminodecaneKocuria rosea--395447 2D-image
1,12-diaminododecaneKocuria rosea--395447 2D-image
1,3-DiaminopropaneKocuria rosea--395447 2D-image
1,3-DiaminopropaneRhodococcus erythropolisB0F9F6competitive inhibitor684634 2D-image
1,6-diaminohexaneKocuria rosea--395447 2D-image
1,7-DiaminoheptaneKocuria rosea--395447 2D-image
1,8-diaminooctaneKocuria rosea--395447 2D-image
1-aminoethanolKocuria rosea--395447 2D-image
1-ethyl-3-(3-dimethylaminopropyl)carbodiimideKocuria rosea-the activity of the modified enzyme towards putrescine is 5.6% of that of the native enzyme. The modified enzyme shows activity towards monoamines such as n-butylamine, n-hexylamine and n-octylamine, which are not substrates of the native enzyme395448 2D-image
2-AminoethanolKocuria rosea--395447 2D-image
3-Amino-1-propanolKocuria rosea--395447 2D-image
4-amino-1-butanoic acidKocuria rosea--395447 2D-image
5-amino-1-pentanoic acidKocuria rosea--395447 2D-image
5-amino-1-pentanolKocuria rosea--395447 2D-image
6-amino-1-hexanoic acidKocuria rosea--395447-
6-amino-1-hexanolKocuria rosea--395447 2D-image
8-amino-1-octanoic acidKocuria rosea--395447 2D-image
agmatineKocuria rosea--395447 2D-image
allylamineKocuria rosea--395447 2D-image
AminoethanolRhodococcus erythropolisB0F9F6competitive inhibitor684634 2D-image
AminoguanidineBos taurus--395442 2D-image
Aminoguanidine hydrogen carbonateBos taurus--395442 2D-image
ammoniumKocuria rosea--395447 2D-image
benzylamineKocuria rosea--395447 2D-image
ButylamineKocuria rosea--395447 2D-image
cadaverineKocuria rosea-noncompetitive against putrescine395438 2D-image
Cd2+Kocuria rosea-inactivation due to dissociation of FAD from the enzyme molecule and denaturation of the apoenzyme395448 2D-image
Co2+Kocuria rosea-inactivation due to dissociation of FAD from the enzyme molecule and denaturation of the apoenzyme395448 2D-image
Cu2+Kocuria rosea-inactivation due to dissociation of FAD from the enzyme molecule and denaturation of the apoenzyme395448 2D-image
cyanideKocuria rosea--395437 2D-image
dodecylamineKocuria rosea--395447 2D-image
ethylamineKocuria rosea--395447 2D-image
ethylenediamineKocuria rosea--395447 2D-image
ethylenediamineRhodococcus erythropolisB0F9F6competitive inhibitor684634 2D-image
heptylamineKocuria rosea--395447 2D-image
HexylamineKocuria rosea--395447 2D-image
Hg2+Kocuria rosea-inactivation due to dissociation of FAD from the enzyme molecule and denaturation of the apoenzyme395448 2D-image
hydroxylamineKocuria rosea--395437 2D-image
IproniazidKocuria rosea--395437 2D-image
MethylamineKocuria rosea--395447 2D-image
N,N,N',N'-tetramethyl-1,4-diaminobutaneKocuria rosea--395447 2D-image
n-butylamineRhodococcus erythropolisB0F9F6competitive inhibitor684634 2D-image
Ni2+Kocuria rosea-inactivation due to dissociation of FAD from the enzyme molecule and denaturation of the apoenzyme395448 2D-image
PCMBKocuria rosea--395437, 395450 2D-image
PentylamineKocuria rosea--395447 2D-image
PhenylethylamineKocuria rosea--395447 2D-image
PropylamineKocuria rosea--395447 2D-image
putrescinePseudomonas putida-substrate inhibition at 10 mM or above288077 2D-image
spermidineKocuria rosea-competitive inhibition of putrescine oxidation395446 2D-image
spermineKocuria rosea-competitive inhibition of putrescine oxidation395446 2D-image
tyramineKocuria rosea--395447 2D-image
Zn2+Kocuria rosea-inactivation due to dissociation of FAD from the enzyme molecule and denaturation of the apoenzyme395448 2D-image
methylglyoxal bis(guanylhydrazine)dihydrochloride monohydrateBos taurus--395442 2D-image
additional informationRhodococcus erythropolisB0F9F6short diamines and monoamines strongly inhibit activity684634-

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.17-1,6-diaminohexaneRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C684634 2D-image
0.065-2-hydroxyputrescineRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C684634 2D-image
0.018-cadaverineRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C684634 2D-image
1.1-cadaverineMeyerozyma guilliermondii--395444 2D-image
0.33-L-ornithineRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C684634 2D-image
0.124-O2Kocuria rosea--395438 2D-image
0.0026-putrescineBos taurus--395442 2D-image
0.0035-putrescineRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C684634 2D-image
0.0082-putrescineRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C684634 2D-image
0.02-putrescineMeyerozyma guilliermondii--395444 2D-image
0.03-putrescinePseudomonas putida--288077 2D-image
0.038-putrescineKocuria rosea--395438 2D-image
0.125-putrescineKocuria rosea--395446 2D-image
0.23-putrescineKocuria rosea--395437 2D-image
3.3-putrescineEmericella nidulans--395441 2D-image
110-putrescineRhodococcus erythropolisB0F9F6mutant enzyme E324A, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C; mutant enzyme E324L, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C684634 2D-image
0.025-spermidineBos taurus--395442 2D-image
0.1-spermidineRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C684634 2D-image
0.2-spermidineMeyerozyma guilliermondii--395444 2D-image
0.23-spermidineKocuria rosea--395437 2D-image
0.47-spermineRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C684634 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.37-1,6-diaminohexaneRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C684634 2D-image
7.5-2-hydroxyputrescineRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C684634 2D-image
3.9-cadaverineRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C684634 2D-image
0.0198-L-ornithineRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C684634 2D-image
3.2-putrescineRhodococcus erythropolisB0F9F6mutant enzyme E324L, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C684634 2D-image
6.1-putrescineRhodococcus erythropolisB0F9F6mutant enzyme E324A, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C684634 2D-image
20.7-putrescineRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C684634 2D-image
26.4-putrescineRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C684634 2D-image
1.4-spermidineRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C684634 2D-image
0.18-spermineRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C684634 2D-image

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
5.8-(2-aminoethyl)-trimethylammoniumRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C684634 2D-image
0.19-1,1,4,4-tetramethyl-1,4-diaminobutaneKocuria rosea--395447 2D-image
0.003-1,10-DiaminodecaneKocuria rosea--395447 2D-image
0.002-1,12-diaminododecaneKocuria rosea--395447 2D-image
0.064-1,3-DiaminopropaneRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C684634 2D-image
0.12-1,3-DiaminopropaneKocuria rosea--395447 2D-image
0.023-1,6-diaminohexaneKocuria rosea--395447 2D-image
0.0082-1,7-DiaminoheptaneKocuria rosea--395447 2D-image
0.0032-1,8-diaminooctaneKocuria rosea--395447 2D-image
3-2-AminoethanolKocuria rosea--395447 2D-image
0.5-3-Amino-1-propanolKocuria rosea--395447 2D-image
0.67-3-Amino-1-propanolKocuria rosea--395447 2D-image
440-4-amino-1-butanoic acidKocuria rosea--395447 2D-image
59-5-amino-1-pentanoic acidKocuria rosea--395447 2D-image
0.088-5-amino-1-pentanolKocuria rosea--395447 2D-image
300-6-amino-1-hexanoic acidKocuria rosea--395447-
0.31-6-amino-1-hexanolKocuria rosea--395447 2D-image
160-8-amino-1-octanoic acidKocuria rosea--395447 2D-image
0.036-agmatineKocuria rosea--395447 2D-image
0.013-allylamineKocuria rosea--395447 2D-image
0.0018-AminoethanolRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C684634 2D-image
2.2e-05-AminoguanidineBos taurus-with putrescine as substrate395442 2D-image
20-ammoniumKocuria rosea--395447 2D-image
0.047-benzylamineKocuria rosea--395447 2D-image
0.46-ButylamineKocuria rosea--395447 2D-image
0.28-cadaverineKocuria rosea--395438 2D-image
0.0082-dodecylamineKocuria rosea--395447 2D-image
2.3-ethylamineKocuria rosea--395447 2D-image
0.66-ethylenediamineRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C684634 2D-image
0.95-ethylenediamineKocuria rosea--395447 2D-image
0.014-heptylamineKocuria rosea--395447 2D-image
0.039-HexylamineKocuria rosea--395447 2D-image
3.4-MethylamineKocuria rosea--395447 2D-image
2.4e-05-methylglyoxal bis(guanylhydrazine)dihydrochloride monohydrateBos taurus--395442 2D-image
0.14-N,N,N',N'-tetramethyl-1,4-diaminobutaneKocuria rosea--395447 2D-image
0.12-n-butylamineRhodococcus erythropolisB0F9F6wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C684634 2D-image
0.24-PentylamineKocuria rosea--395447 2D-image
0.087-PhenylethylamineKocuria rosea--395447 2D-image
0.62-PropylamineKocuria rosea--395447 2D-image
0.181-spermidineKocuria rosea--395446 2D-image
0.0581-spermineKocuria rosea--395446 2D-image
0.039-tyramineKocuria rosea--395447 2D-image

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
9.1-Kocuria rosea--395450
35-Kocuria rosea--395436
59.16-Kocuria rosea--395438
additional information-Pseudomonas putida--288077

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
811.7Pseudomonas putida--288077
8-Kocuria rosea-putrescine395437
8-Meyerozyma guilliermondii--395444
8-Bos taurus--395449
8-Rhodococcus erythropolisB0F9F6around pH 8.0684634
8.5-Kocuria rosea-spermidine395437, 395450

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
6.89Meyerozyma guilliermondii-pH 6.8: about 50% of maximal activity, pH 9.0: about 60% of maximal activity395444

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
37-Meyerozyma guilliermondii--395444

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
2050Meyerozyma guilliermondii-20°C: about 45% of maximal activity, 50°C: about 50% of maximal activity395444

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
commercial preparationKocuria rosea--654187Manually annotated by BRENDA team
ovaryPisum sativum-ovary senescence is characterized by increased level of putrescine oxidase, constant level of putrescine oxidase during fruit development395445Manually annotated by BRENDA team
serumBos taurus-absent from bovine serum after birth; foetal395442Manually annotated by BRENDA team
serumBos taurus-foetal395449Manually annotated by BRENDA team
fruitPisum sativum--395445Manually annotated by BRENDA team
additional informationMicrococcus luteus-high level of enzyme is produced in cells from the late exponential growth phase395440Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
No entries in this field

PDBSCOPCATHORGANISM
3rha, downloadSCOP (3rha)CATH (3rha)Arthrobacter aurescens (strain TC1)
2yg3, downloadSCOP (2yg3)CATH (2yg3)Rhodococcus erythropolis
2yg4, downloadSCOP (2yg4)CATH (2yg4)Rhodococcus erythropolis
2yg5, downloadSCOP (2yg5)CATH (2yg5)Rhodococcus erythropolis
2yg6, downloadSCOP (2yg6)CATH (2yg6)Rhodococcus erythropolis
2yg7, downloadSCOP (2yg7)CATH (2yg7)Rhodococcus erythropolis

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
8340087000Kocuria rosea-calculation from sedimentation data395438
90000-Kocuria rosea-gel filtration395438
98750-Rhodococcus erythropolisB0F9F6theoretical molecular mass684634
100100-Rhodococcus erythropolis-mass spectrometry687763
108000-Rhodococcus erythropolisB0F9F6SDS-PAGE684634

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
?Kocuria rosea-x * 50000, SDS-PAGE395443
dimerKocuria rosea-2 * 46000, SDS-PAGE395438
homodimerRhodococcus erythropolisB0F9F62 * 54000, SDS-PAGE684634
homodimerRhodococcus erythropolis--687763

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
610.5Pseudomonas putida-stable288077
6.4-Rhodococcus erythropolisB0F9F6below pH 6.4, no significant activity can be detected684634

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
3750Rhodococcus erythropolisB0F9F6at 50°C, half of the activity is lost after 2 h, while at 37°C, such a degree of inactivation is reached after 1 day684634
40-Pseudomonas putida-pH 7.5, 20 min, 17% loss of activity288077
40-Kocuria rosea-stable up to395437
50-Pseudomonas putida-pH 7.5, 20 min, 62% loss of activity288077
50-Kocuria rosea-rapid inactivation at395437
60-Pseudomonas putida-pH 7.5, 20 min, 93% loss of activity288077
additional information-Kocuria rosea-the enzyme is resistant to heat denaturation as long as it is maintained in a fully reduced state395438

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
photoreduction of the enzyme in presence of EDTAKocuria rosea-395438

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-22°C, stable for monthsKocuria rosea-395437
5°C, 0.01 M phosphate buffer, pH 6.0-8.0, little loss of activityKocuria rosea-395437
-20°C, in presence of 25% glycerol, 1 year, stablePseudomonas putida-288077

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Bos taurus-395449
-Kocuria rosea-395436, 395437, 395438, 395450
partialMeyerozyma guilliermondii-395444
-Rhodococcus erythropolis-687763
Q-Sepharose column chromatographyRhodococcus erythropolisB0F9F6684634

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
expression in Escherichia coliKocuria rosea-395443
expressed in Escherichia coli Top10 cellsRhodococcus erythropolis-684634, 687763

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
A394CRhodococcus erythropolis-active mutant with covalently bound FAD687763
E324ARhodococcus erythropolisB0F9F6the mutant enzyme shows activity with monoamines, which are not accepted by the wild type enzyme684634
E324LRhodococcus erythropolisB0F9F6the mutant enzyme shows activity with monoamines, which are not accepted by the wild type enzyme684634

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
analysisKocuria rosea-differential determination procedure for putrescine, spermidine and spermine395446
analysisKocuria rosea-enzyme can be used as biosensor for putrescine using dispersed multiwalled carbon nanotubes as ultra-microelectrodes ensuring a good electron transfer from the enzyme, method optimization, overview654187
diagnosticsKocuria rosea-enzyme can be used as biosensor for putrescine using dispersed multiwalled carbon nanotubes as ultra-microelectrodes ensuring a good electron transfer from the enzyme, method optimization, overview654187
analysisPseudomonas putida-specific measurement of putrescine with putrescine oxidase and aminobutyraldehyde dehydrogenase288077

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
288077Shimizu, E.; Tabata, Y.; Hayakawa, R.; Yorifuji, T.Specific measurement of putrescine with putrescine oxidase and aminobutyraldehyde dehydrogenaseAgric. Biol. Chem.522865-28711988Pseudomonas putida-
395436Okada, M.; Kawashima, S.; Imahori, K.Purification of putrescine oxidase from Micrococcus rubens by affinity chromatographyMethods Enzymol.94301-3031983Kocuria rosea PubMed
395437Yamada, H.Putrescine oxidase (Micrococcus rubens)Methods Enzymol.17B726-7301971Kocuria rosea-
395438Desa, R.J.Putrescine oxidase from Micrococcus rubens. Purification and properties of the enzymeJ. Biol. Chem.2475527-55341972Kocuria rosea PubMed
395440Rokka, R.; Futamura, N.; Ishida, A.Role of putrescine oxidase in the generation of hydrogen peroxide by Micrococcus luteusJ. Gen. Appl. Microbiol.31435-4401985Micrococcus luteus-
395441Spathas, D.H.; Clutterbuck, A.J.; Pateman, J.A.Putrescine as a nitrogen source for wild type and mutants of Aspergillus nidulansFEMS Microbiol. Lett.17345-3481983Emericella nidulans-
395442Gahl, W.A.; Pitot, H.C.Polyamine degradation in foetal and adult bovine serumBiochem. J.202603-6111982Bos taurus PubMed
395443Ishizuka, H.; Horinouchi, S.; Beppu, T.Putrescine oxidase of Micrococcus rubens: primary structure and Escherichia coliJ. Gen. Microbiol.139425-432.1993Kocuria rosea PubMed
395444Gunasekaran, M.; Gunasekaran, U.Partial purification and properties of putrescine oxidase from Candida guilliermondiiAppl. Biochem. Biotechnol.76229-2361999Candida albicans, Candida parapsilosis, Candida tropicalis, Meyerozyma guilliermondii, Pichia kudriavzevii PubMed
395445Perez-Amador, M.A.; Carbonell, J.Arginine decarboxylase and putrescine oxidase in ovaries of Pisum sativum L.: changes during ovary senescence and early stages of fruit developmentPlant Physiol.107865-8721995Pisum sativum PubMed
395446Isobe, K.; Tani, Y.; Yamada, H.Differential determination procedure for putrescine, spermidine and spermine with polymaine oxidase from fungi and putrescine oxidaseAgric. Biol. Chem.45727-7331981Kocuria rosea PubMed
395447Swain, W.F.; Desa, R.J.Mechanism of action of putrescine oxidase. Binding characteristics of the active site of putrescine oxidase from Micrococcus rubensBiochim. Biophys. Acta429331-3411976Kocuria rosea PubMed
395448Okada, M.; Kawashima, S.; Imahori, K.Mode of inactivation of putrescine oxidase by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide or metal ionsJ. Biochem.88481-4881980Kocuria rosea PubMed
395449Gahl, W.A.; Vale, A.M.; Pitot, H.C.Separation of putrescine oxidase and spermidine oxidase in foetal bovine serum with the aid of a specific radioactive assay of spermidine oxidaseBiochem. J.187197-2041980Bos taurus PubMed
395450Adachi, O.; Yamada, H.; Ogata, K.Purification and properties of putrescine oxidase of Micrococcus rubensAgric. Biol. Chem.301202-12101966Kocuria rosea-
654187Rochette, J.F.; Sacher, E.; Meunier, M.; Luong, J.H.A mediatorless biosensor for putrescine using multiwalled carbon nanotubesAnal. Biochem.336305-3112005Kocuria rosea PubMed
684634van Hellemond, E.W.; van Dijk, M.; Heuts, D.P.; Janssen, D.B.; Fraaije, M.W.Discovery and characterization of a putrescine oxidase from Rhodococcus erythropolis NCIMB 11540Appl. Microbiol. Biotechnol.78455-4632008Rhodococcus erythropolis PubMed
687763van Hellemond, E.W.; Mazon, H.; Heck, A.J.; van den Heuvel, R.H.; Heuts, D.P.; Janssen, D.B.; Fraaije, M.W.ADP competes with FAD binding in putrescine oxidaseJ. Biol. Chem.28328259-282642008Rhodococcus erythropolis PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 1.4.3.10)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)