Information on EC 1.14.99.2 - kynurenine 7,8-hydroxylase:

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The expected taxonomic range for this enzyme is: Proteobacteria


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EC NUMBERCOMMENTARY
1.14.99.2-

RECOMMENDED NAMEGeneOntology No.
kynurenine 7,8-hydroxylaseGO:0050016

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
kynurenate + AH2 + O2 = 7,8-dihydro-7,8-dihydroxykynurenate + A
show the reaction diagram
----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
oxidation----
redox reaction----
reduction----

PATHWAYKEGG LinkMetaCyc Link
Tryptophan metabolism00380 -

SYSTEMATIC NAMEIUBMB Comments
kynurenate,hydrogen-donor:oxygen oxidoreductase (hydroxylating)-

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
ec 1.14.1.4--formerly-
hydroxylase, kynurenate----
hydroxylase, kynurenate 7,8-----
kynurenate 7,8-hydroxylase----
kynurenic acid hydroxylase----
kynurenic hydroxylase----

CAS REGISTRY NUMBERCOMMENTARY
9029-63-4-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Pseudomonas fluorescensATCC 11299b390584--Manually annotated by BRENDA team
Pseudomonas sp.overview396602--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
kynurenate + NADH + O27,8-dihydro-7,8-dihydroxykynurenate + NAD+
show the reaction diagram
Pseudomonas fluorescens--390584--?
kynurenate + NADH + O27,8-dihydro-7,8-dihydroxykynurenate + NAD+
show the reaction diagram
Pseudomonas sp.--396602--?
kynurenate + NADPH + O27,8-dihydro-7,8-dihydroxykynurenate + NADP+
show the reaction diagram
Pseudomonas fluorescens--390584--?
kynurenate + NADPH + O27,8-dihydro-7,8-dihydroxykynurenate + NADP+
show the reaction diagram
Pseudomonas sp.--396602--?

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
kynurenate + NADH + O27,8-dihydro-7,8-dihydroxykynurenate + NAD+
show the reaction diagram
Pseudomonas fluorescens--390584--?
kynurenate + NADH + O27,8-dihydro-7,8-dihydroxykynurenate + NAD+
show the reaction diagram
Pseudomonas sp.--396602--?
kynurenate + NADPH + O27,8-dihydro-7,8-dihydroxykynurenate + NADP+
show the reaction diagram
Pseudomonas fluorescens--390584--?
kynurenate + NADPH + O27,8-dihydro-7,8-dihydroxykynurenate + NADP+
show the reaction diagram
Pseudomonas sp.--396602--?

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
NADHPseudomonas fluorescens--390584 2D-image
NADHPseudomonas sp.--396602 2D-image
NADPHPseudomonas fluorescens--390584 2D-image

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
No entries in this field

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
Ag+Pseudomonas fluorescens--390584 2D-image
Cu2+Pseudomonas fluorescens--390584 2D-image
Hg2+Pseudomonas fluorescens--390584 2D-image
iodoacetatePseudomonas fluorescens-inhibition reversed by addition of GSH or cysteine390584 2D-image
KynurenatePseudomonas fluorescens-competitive inhibition at 0.2 mM390584 2D-image
p-chloromercuribenzoatePseudomonas fluorescens-inhibition reversed by addition of GSH or cysteine390584 2D-image

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.008-KynurenatePseudomonas fluorescens--390584 2D-image
0.04-NADHPseudomonas fluorescens--390584 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
7-Pseudomonas fluorescens--390584

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
23-Pseudomonas fluorescens-assay at390584

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
No entries in this field

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
No entries in this field

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
9-Pseudomonas fluorescens-stable in presence 10 mM cysteine390584

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
60-Pseudomonas fluorescens-5 min, complete inactivation390584

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
2-mercaptoethanol stabilizes activityPseudomonas fluorescens-390584
cysteine stabilizes activityPseudomonas fluorescens-390584
GSH stabilizes activityPseudomonas fluorescens-390584
precipitation of supernatant with ammonium sulfate in the absence of cysteine, complete loss of activityPseudomonas fluorescens-390584
thioglycolate stabilizes activityPseudomonas fluorescens-390584

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-10C, ammonium sulfate fractions, loss of activity after a few daysPseudomonas fluorescens-390584
-10C, supernatant after high speed centrifugation of crude extract, several months, no loss in activityPseudomonas fluorescens-390584

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
partialPseudomonas fluorescens-390584
-Pseudomonas sp.-396602

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISM (UNIPROT ACCESSION NO.)LINK TO PUBMEDSOURCE
390584Taniuchi, H.; Hayaishi, O.Studies on the metabolism of kynurenic acidJ. Biol. Chem.238283-2931963Pseudomonas fluorescens PubMed
396602Gibson, D.T.Assay of enzymes of aromatic metabolismMethods Microbiol.6A463-4781971Pseudomonas sp.-

LINKS TO OTHER DATABASES (specific for EC-Number 1.14.99.2)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)