Information on EC 1.14.11.16 -

Information on EC 1.14.11.16 - peptide-aspartate beta-dioxygenase:

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EC NUMBER	COMMENTARY
1.14.11.16	-

RECOMMENDED NAME	GeneOntology No.
peptide-aspartate beta-dioxygenase	GO:0004597

REACTION	REACTION DIAGRAM	COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
peptide-L-aspartate + 2-oxoglutarate + O2 = peptide-3-hydroxy-L-aspartate + succinate + CO2		-	-	-	-

REACTION TYPE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
decarboxylation	Homo sapiens	-	-	699589
hydroxylation	-	-	-	-
hydroxylation	Homo sapiens	-	-	676817, 699589
oxidation	-	-	-	-
redox reaction	-	-	-	-
reduction	-	-	-	-

PATHWAY	KEGG Link	MetaCyc Link
No entries in this field

SYSTEMATIC NAME	IUBMB Comments
peptide-L-aspartate,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)	Requires Fe2+. Some vitamin K-dependent coagulation factors, as well as synthetic peptides based on the structure of the first epidermal growth factor domain of human coagulation factor IX or X, can act as acceptors.

SYNONYMS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
AAH	Homo sapiens	-	-	673915, 673951, 675183
AAH	Rattus norvegicus	-	-	695432
AAH	Mus musculus	-	-	698158
aspartate beta-hydroxylase	-	-	-	-
aspartyl (asparaginyl) beta-hydroxylase	Homo sapiens	-	-	698201
aspartyl (asparaginyl)-beta-hydroxylase	Homo sapiens	-	-	698195
aspartyl beta-hydroxylase	Homo sapiens	-	-	675861
aspartyl-(asparaginyl) beta-hydroxylase	Homo sapiens	-	-	673951
aspartyl-(asparaginyl)-b-hydroxylase	Homo sapiens	-	-	675183
aspartyl-(asparaginyl)-beta-hydroxylase	Mus musculus	-	-	698158
aspartyl-(asparagyl)-beta-hydroxylase	Homo sapiens	-	-	673915
aspartyl-asparaginyl-beta-hydroxylase	Rattus norvegicus	-	-	695432
aspartylpeptide beta-dioxygenase	-	-	-	-
EGF beta-hydroxylase	Homo sapiens	-	-	657901
EGFH	Homo sapiens	-	-	657901
factor inhibiting HIF	Homo sapiens	-	-	687592
factor inhibiting hypoxia-inducible factor	Homo sapiens	-	-	676817
factor inhibiting hypoxia-inducible transcription factor (HIF)	Homo sapiens	-	-	657901, 659316, 659445
factor-inhibiting hypoxia-inducible factor	Homo sapiens	-	-	699589
FIH	Homo sapiens	-	-	657901, 659316, 659445, 676817, 699589
HAAH	Homo sapiens	-	-	698201
HIF asparaginyl hydroxylase	Homo sapiens	-	-	687592
human aspartyl (asparaginyl) beta-hydroxylase	Homo sapiens	-	-	657901
hypoxia-inducible factor asparaginyl hydroxylase	Homo sapiens	-	-	676817

CAS REGISTRY NUMBER	COMMENTARY
122544-66-5	-

ORGANISM	COMMENTARY	LITERATURE	SEQUENCE CODE	SEQUENCE DB	SOURCE
Bos taurus	-	439253, 439255, 439256, 439258, 439259	-	-
Homo sapiens	-	439252, 439254, 657901, 659316, 673915, 673951, 675183, 675861, 676817, 684228, 687022, 687592, 698195, 698201, 699589	-	-
Homo sapiens	-	659445	Q9NWT6	Uniprot
Homo sapiens	human osteosarcoma	439257	-	-
Mus musculus	-	439260, 687592, 698158	-	-
Mus musculus	three different size transcripts 2.8, 4.5 and 6.6 kb, the bigger ones lead to active proteins after expression	439251	-	-
Rattus norvegicus	-	439260, 695432	-	-

GENERAL INFORMATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
malfunction	Rattus norvegicus	-	inhibition by fetal alcohol spectrum disorder, decrease leads to impairment in neuronal migration	695432
physiological function	Rattus norvegicus	-	mediates neuronal motility	695432
physiological function	Mus musculus	-	stimulates cell migration	698158

SUBSTRATE	PRODUCT	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY/ Substrate	LITERATURE/ Substrate	COMMENTARY/ Product	LITERATURE/ Product	Reversibility r=reversible ir=irreversible ?=not specified
ankyrin repeat domain of endogenous Notch receptor L-asparagine + 2-oxoglutarate + O2	ankyrin repeat domain of endogenous Notch receptor 3-hydroxy-L-asparagine + succinate + CO2		Homo sapiens	-	hydroxylation of highly conserved asparaginyl residues within the ankyrin repeat	687592	-	-	?
ankyrin repeat domain of endogenous Notch receptor L-asparagine + 2-oxoglutarate + O2	ankyrin repeat domain of endogenous Notch receptor 3-hydroxy-L-asparagine + succinate + CO2		Mus musculus	-	hydroxylation of hyghly conserved asparaginyl residues within the ankyrin repeat	687592	-	-	?
ankyrin repeat domain protein	?		Homo sapiens	-	a maximum of three ankyrin repeats enables ankyrin repeat domain proteins as a substrate	699589	-	-	?
HIF (L-Asn803) + 2-oxoglutarate + O2	HIF (3-hydroxy-L-Asn803) + succinate + CO2		Homo sapiens	-	accepts HIF-1alpha and HIF-2-alpha as substrate, HIF mutant V802A exhibits 4fold lower substrate activity than native protein, no activity with N803A mutant	659316	-	-	?
peptide L-asparagine + 2-oxoglutarate + O2	peptide 3-hydroxy-L-asparagine + succinate + CO2		Homo sapiens	-	-	439252, 657901, 659316	-	-	?
peptide L-asparagine + 2-oxoglutarate + O2	peptide 3-hydroxy-L-asparagine + succinate + CO2		Homo sapiens	Q9NWT6	-	659445	-	-	?
peptide L-asparagine + 2-oxoglutarate + O2	peptide 3-hydroxy-L-asparagine + succinate + CO2		Homo sapiens	-	-	673915, 675183, 675861, 676817	-	-	?
peptide L-asparagine + 2-oxoglutarate + O2	peptide 3-hydroxy-L-asparagine + succinate + CO2		Bos taurus	-	-	439253	-	-	?
peptide L-asparagine + 2-oxoglutarate + O2	peptide 3-hydroxy-L-asparagine + succinate + CO2		Homo sapiens	-	first epidermal growth factor-like domain of bovine protein S with an asparagine replacing the aspartic acid at position 18	439254	-	-	?
peptide L-asparagine + 2-oxoglutarate + O2	peptide 3-hydroxy-L-asparagine + succinate + CO2		Bos taurus	-	first epidermal growth factor-like domain of human protein S with an asparagine replacing the aspartic acid at position 18	439256, 439258	-	-	?
peptide L-asparagine + 2-oxoglutarate + O2	peptide 3-hydroxy-L-asparagine + succinate + CO2		Homo sapiens	-	enzyme catalyzes the erythro-beta-hydroxylation of asparaginyl and aspartyl residues to form the 2S, 3R-product, possible role in the Notch signalling pathway, hydroxylation of Asn803 in hypoxia-inducible transcription factor, converted protein is incapable in interacting with the transcripitonal coactivator p300	657901	-	-	?
peptide L-asparagine + 2-oxoglutarate + O2	peptide 3-hydroxy-L-asparagine + succinate + CO2		Homo sapiens	-	hydroxylation of Asn803 in hypoxia-inducible transcription factor, converted protein is incapable in interacting with the transcripitonal coactivator p300	659316	-	-	?
peptide L-asparagine + 2-oxoglutarate + O2	peptide 3-hydroxy-L-asparagine + succinate + CO2		Homo sapiens	Q9NWT6	hydroxylation of Asn803 in hypoxia-inducible transcription factor, converted protein is incapable in interacting with the transcripitonal coactivator p300	659445	-	-	?
peptide L-aspartate + 2-oxoglutarate + O2	peptide 3-hydroxy-L-aspartate + succinate + CO2		Mus musculus	-	-	439251	-	439251	?
peptide L-aspartate + 2-oxoglutarate + O2	peptide 3-hydroxy-L-aspartate + succinate + CO2		Bos taurus	-	-	439253	-	439253	?
peptide L-aspartate + 2-oxoglutarate + O2	peptide 3-hydroxy-L-aspartate + succinate + CO2		Homo sapiens	-	first epidermal growth factor-like domain of bovine protein S as substrate	439252	-	439252	?
peptide L-aspartate + 2-oxoglutarate + O2	peptide 3-hydroxy-L-aspartate + succinate + CO2		Bos taurus	-	first epidermal growth factor-like domain of human protein S as substrate	439258	-	439258	?
peptide L-aspartate + 2-oxoglutarate + O2	peptide 3-hydroxy-L-aspartate + succinate + CO2		Bos taurus	-	first epidermal growth factor-like domain of human protein S as substrate	439259	-	439259	?
peptide L-aspartate + 2-oxoglutarate + O2	peptide 3-hydroxy-L-aspartate + succinate + CO2		Mus musculus, Rattus norvegicus	-	specific erythro-hydroxylation	439260	-	439260	?
peptide L-aspartate + 2-oxoglutarate + O2	peptide 3-hydroxy-L-aspartate + succinate + CO2		Bos taurus	-	specific erythro-hydroxylation	439259	-	439259	?
peptide L-aspartate + 2-oxoglutarate + O2	peptide 3-hydroxy-L-aspartate + succinate + CO2		Mus musculus, Rattus norvegicus	-	first epidermal growth factor-like domain of human factor IX as substrate	439260	-	439260	?
peptide L-aspartate + 2-oxoglutarate + O2	peptide 3-hydroxy-L-aspartate + succinate + CO2		Bos taurus	-	second epidermal growth factor-like domain of bovine protein S	439255	-	439255	?
peptide L-aspartate + 2-oxoglutarate + O2	peptide 3-hydroxy-L-aspartate + succinate + CO2		Homo sapiens	-	hydroxylates epidermal growth factor-like domains in transformation-associated proteins	439252	-	439252	?
HIF-1alpha peptide Asp788-Leu822 (L-Asn803) + 2-oxoglutarate + O2	HIF-1alpha peptide Asp788-Leu822 (3-hydroxy-L-Asn803) + succinate + CO2		Homo sapiens	Q9NWT6	much lower activity with peptides lacking residues 819-822, 807-822, and 815-822	659445	-	-	?
additional information	?	-	Mus musculus	-	overexpression may be associated with malignant transformation	439251	-	-	-
additional information	?	-	Homo sapiens	-	overexpression may be associated with malignant transformation	439252	-	-	-

NATURAL SUBSTRATES	NATURAL PRODUCTS	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY SUBSTRATE	LITERATURE (Substrate)	COMMENTARY PRODUCT	LITERATURE (Product)
peptide L-asparagine + 2-oxoglutarate + O2	peptide 3-hydroxy-L-asparagine + succinate + CO2		Homo sapiens	-	enzyme catalyzes the erythro-beta-hydroxylation of asparaginyl and aspartyl residues to form the 2S, 3R-product, possible role in the Notch signalling pathway, hydroxylation of Asn803 in hypoxia-inducible transcription factor, converted protein is incapable in interacting with the transcripitonal coactivator p300	657901	-	-
peptide L-asparagine + 2-oxoglutarate + O2	peptide 3-hydroxy-L-asparagine + succinate + CO2		Homo sapiens	-	hydroxylation of Asn803 in hypoxia-inducible transcription factor, converted protein is incapable in interacting with the transcripitonal coactivator p300	659316	-	-
peptide L-asparagine + 2-oxoglutarate + O2	peptide 3-hydroxy-L-asparagine + succinate + CO2		Homo sapiens	Q9NWT6	hydroxylation of Asn803 in hypoxia-inducible transcription factor, converted protein is incapable in interacting with the transcripitonal coactivator p300	659445	-	-
peptide L-aspartate + 2-oxoglutarate + O2	peptide 3-hydroxy-L-aspartate + succinate + CO2		Mus musculus	-	-	439251	-	439251
peptide L-aspartate + 2-oxoglutarate + O2	peptide 3-hydroxy-L-aspartate + succinate + CO2		Homo sapiens	-	hydroxylates epidermal growth factor-like domains in transformation-associated proteins	439252	-	439252
additional information	?	-	Mus musculus	-	overexpression may be associated with malignant transformation	439251	-	-
additional information	?	-	Homo sapiens	-	overexpression may be associated with malignant transformation	439252	-	-

COFACTOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

METALS and IONS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
Fe2+	Bos taurus	-	-	439255, 439256, 439258, 439259
Fe2+	Mus musculus	-	0.05 mM increases activity 6-fold	439260
Fe2+	Rattus norvegicus	-	-	439260
Fe2+	Homo sapiens	-	required	657901, 659316, 659445

INHIBITORS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
2,2'-dipyridyl	Mus musculus	-	at 1 mM 90% inhibition	439260	2D-image
ankyrin repeat domain	Homo sapiens, Mus musculus	-	may function as a natural inhibitor and provide an oxygen-dependent interface that modulates hypoxia-induced factor signaling	687592	-
dimethyloxalylglycine	Homo sapiens	-	-	676817	2D-image
ethanol	Rattus norvegicus	-	-	695432	2D-image
Insulin-like growth factor-1	Homo sapiens	-	stimulates peptide-aspartate beta-dioxygenase protein expression and directional motility. Ethanol reduces the stimulation without inhibition of the mRNA expression	684228	-
iodoacetamide	Bos taurus	-	at 1 mM less than 5% activity, 2-oxoglutarate and EDTA protects	439256	2D-image
additional information	Homo sapiens	Q9NWT6	several 2-oxoglutarate analogs inhibits enzyme activity	659445	-
additional information	Rattus norvegicus	-	phosphorylation by GSK-3beta oder degradation by caspase	695432	-

ACTIVATING COMPOUND	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
ascorbate	Homo sapiens	Q9NWT6	required	659445	2D-image
additional information	Rattus norvegicus	-	regulated by insulin and insulin-like growth factor	695432	-

KM VALUE [mM]	KM VALUE [mM] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
0.005	-	2-oxoglutarate	Bos taurus	-	-	439259	2D-image
0.021	-	2-oxoglutarate	Bos taurus	-	56 kDa protein	439258	2D-image
0.022	-	2-oxoglutarate	Bos taurus	-	52 kDa protein	439258	2D-image
0.025	-	2-oxoglutarate	Homo sapiens	Q9NWT6	pH 7.8, 30�C	659445	2D-image
0.098	-	2-oxoglutarate	Bos taurus	-	at 1 mM Fe2+, H675D mutant	439255	2D-image
0.102	-	2-oxoglutarate	Bos taurus	-	at 1 mM Fe2+, wild-type	439255	2D-image
0.125	-	2-oxoglutarate	Bos taurus	-	at 1 mM Fe2+, H675E mutant	439255	2D-image
0.003	-	Fe2+	Bos taurus	-	-	439259	2D-image
0.0083	-	Fe2+	Bos taurus	-	56 kDa protein	439258	2D-image
0.0096	-	Fe2+	Bos taurus	-	52 kDa protein	439258	2D-image
0.013	-	Fe2+	Bos taurus	-	at 0.6 mM 2-oxoglutarate, wild-type	439255	2D-image
0.052	-	Fe2+	Bos taurus	-	at 0.6 mM 2-oxoglutarate, H675E mutant	439255	2D-image
0.093	-	Fe2+	Bos taurus	-	at 0.6 mM 2-oxoglutarate, H675D mutant	439255	2D-image
0.019	-	first epidermal growth factor-like domain	Bos taurus	-	substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, native enzyme	439256	-
0.024	-	first epidermal growth factor-like domain	Bos taurus	-	substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, recombinant enzyme	439256	-
0.034	-	first epidermal growth factor-like domain	Bos taurus	-	substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, 52 kDa protein	439258	-
0.035	-	first epidermal growth factor-like domain	Bos taurus	-	substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, 56 kDa protein	439258	-
0.067	-	first epidermal growth factor-like domain	Bos taurus	-	substrate is of human protein S, 52 kDa protein	439258	-
0.075	-	first epidermal growth factor-like domain	Bos taurus	-	substrate is of human protein S, 56 kDa protein	439258	-
0.1	-	HIF-1alpha peptide Asp788-Leu822 (L-asparagine803)	Homo sapiens	Q9NWT6	pH 7.8, 30�C	659445	-
0.09	-	O2	Homo sapiens	Q9NWT6	pH 7.8, 30�C	659445	2D-image

TURNOVER NUMBER [1/s]	TURNOVER NUMBER MAXIMUM[1/s]	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
0.0167	-	first epidermal growth factor-like domain	Bos taurus	-	substrate is of human protein S, 56 kDa protein	439258	-
0.0217	-	first epidermal growth factor-like domain	Bos taurus	-	substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, 56 kDa protein	439258	-
0.025	-	first epidermal growth factor-like domain	Bos taurus	-	substrate is of human protein S, 52 kDa protein	439258	-
0.0317	-	first epidermal growth factor-like domain	Bos taurus	-	substrate is of human protein S with an asparagine replacing the aspartic acid at position 18, 52 kDa protein	439258	-

kcat/KM VALUE [1/mMs^-1]	kcat/KM VALUE [1/mMs^-1] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

Ki VALUE [mM]	Ki VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

IC50 VALUE [mM]	IC50 VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg]	SPECIFIC ACTIVITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
0.59	-	Bos taurus	-	-	439258
14	-	Bos taurus	-	-	439256

pH OPTIMUM	pH MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
6.8	-	Bos taurus	-	-	439259
7.6	-	Homo sapiens	-	assay at	699589

pH RANGE	pH RANGE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
6.5	7.9	Bos taurus	-	about half-maximal activity at pH 6.5 and 7.9	439259

TEMPERATURE OPTIMUM	TEMPERATURE OPTIMUM MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
additional information	-	Homo sapiens	-	assay at room temperature	699589

TEMPERATURE RANGE	TEMPERATURE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

pI VALUE	pI VALUE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

SOURCE TISSUE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	SOURCE
adipose	Mus musculus	-	-	439251
adrenal gland	Mus musculus	-	-	439251
adrenal gland	Homo sapiens	-	-	439254
bile	Homo sapiens	-	proliferating ducts	439252
brain	Mus musculus	-	-	439251
breast cancer cell	Homo sapiens	-	highly expressed	439254
carcinoma cell line	Homo sapiens	-	hepatocellular carcinoma	698201
cerebellum	Rattus norvegicus	-	of the fetus	695432
cholangiocarcinoma cell	Homo sapiens	-	highly expressed in all cholangiocarcinomas	439254
colonic carcinoma cell	Homo sapiens	-	highly expressed	439254
decidua	Homo sapiens	-	-	673951
endometrial cell line	Homo sapiens	-	-	673951
heart	Mus musculus	-	-	439251
hepatoma cell	Homo sapiens	-	highly expressed in 4 of 10 hepatocarcinomas, 10-fold activity increase	439254
hepatoma cell	Homo sapiens	-	-	673915, 675183, 675861
kidney	Mus musculus	-	-	439251
large cell lung cancer cell line	Homo sapiens	-	-	698195
large intestine	Mus musculus	-	-	439251
liver	Mus musculus	-	-	439251, 439260
liver	Homo sapiens	-	-	439254, 675183, 675861
liver	Bos taurus	-	-	439258, 439259
liver	Rattus norvegicus	-	-	439260
lung	Mus musculus	-	-	439251
lung adenocarcinoma cell line	Homo sapiens	-	-	698195
lung squamous cell carcinoma cell line	Homo sapiens	-	-	698195
neuronal cell line	Rattus norvegicus	-	of the fetus	695432
non-small cell lung cancer cell line	Homo sapiens	-	-	698195
ovary	Mus musculus	-	-	439251
pancreas	Mus musculus	-	-	439251
placenta	Homo sapiens	-	-	673951
PNET-2 cell	Homo sapiens	-	cerebellar neuronal cell	684228
pup	Rattus norvegicus	-	-	695432
skeletal muscle	Mus musculus	-	-	439251
stomach	Mus musculus	-	-	439251
testis	Mus musculus	-	-	439251
thymus	Mus musculus	-	-	439251
trophoblast	Homo sapiens	-	-	673951

LOCALIZATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	GeneOntology No.	LITERATURE	SOURCE
cytoplasm	Homo sapiens	-	-	5737	659316
rough endoplasmic reticulum	Bos taurus	-	-	5791	439259
microsome	Rattus norvegicus	-	-	-	439260
additional information	Mus musculus	-	L-cell extract	-	439260

PDB	SCOP	CATH	ORGANISM
1h2n, download	SCOP (1h2n)	CATH (1h2n)	Homo sapiens
1yci, download	SCOP (1yci)	CATH (1yci)	Homo sapiens
2cgn, download	SCOP (2cgn)	CATH (2cgn)	Homo sapiens
2cgo, download	SCOP (2cgo)	CATH (2cgo)	Homo sapiens
2ilm, download	SCOP (2ilm)	CATH (2ilm)	Homo sapiens
2w0x, download	SCOP (2w0x)	CATH (2w0x)	Homo sapiens
2wa3, download	SCOP (2wa3)	CATH (2wa3)	Homo sapiens
2wa4, download	SCOP (2wa4)	CATH (2wa4)	Homo sapiens
2xum, download	SCOP (2xum)	CATH (2xum)	Homo sapiens
2y0i, download	SCOP (2y0i)	CATH (2y0i)	Homo sapiens
2yc0, download	SCOP (2yc0)	CATH (2yc0)	Homo sapiens
2yde, download	SCOP (2yde)	CATH (2yde)	Homo sapiens
3d8c, download	SCOP (3d8c)	CATH (3d8c)	Homo sapiens
3kcx, download	SCOP (3kcx)	CATH (3kcx)	Homo sapiens
3kcy, download	SCOP (3kcy)	CATH (3kcy)	Homo sapiens
3od4, download	SCOP (3od4)	CATH (3od4)	Homo sapiens
3p3n, download	SCOP (3p3n)	CATH (3p3n)	Homo sapiens
3p3p, download	SCOP (3p3p)	CATH (3p3p)	Homo sapiens
3rcq, download	SCOP (3rcq)	CATH (3rcq)	Homo sapiens
4ai8, download	SCOP (4ai8)	CATH (4ai8)	Homo sapiens

MOLECULAR WEIGHT	MOLECULAR WEIGHT MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
28000	-	Homo sapiens	-	SDS-PAGE	698201
50700	-	Bos taurus	-	sedimentation equilibrium	439256
51030	-	Bos taurus	-	MALDI-TOF	439256
52000	-	Bos taurus	-	SDS-PAGE, protein with lower molecular weight	439258
56000	-	Homo sapiens	-	transfected enzyme, SDS-PAGE	439257
56000	-	Bos taurus	-	SDS-PAGE, protein with higher molecular weight	439258
86000	-	Homo sapiens	-	-	675183
86000	-	Rattus norvegicus	-	Western blot, full-length protein	695432
110000	-	Rattus norvegicus	-	Western blot, post-translational modified protein	695432

SUBUNITS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
dimer	Homo sapiens	-	homodimer, crystal structure analysis	657901
dimer	Homo sapiens	Q9NWT6	homodimer	659445
monomer	Bos taurus	-	1 * 50700, sedimentation equilibrium	439256

POSTTRANSLATIONAL MODIFICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
phosphoprotein	Homo sapiens	-	peptide-aspartate beta-dioxygenase can be phosphorylated by glycogen synthase kinase 3beta, and high levels of glycogen synthase kinase 3beta activity result in decrease in peptide-aspartate beta-dioxygenase protein, whereas inhibition of the kinase and/or global caspases increases peptide-aspartate beta-dioxygenase protein. Phosphorylation is higher in ethanol-treated compared with control cells	684228

Crystallization/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
enzyme in complex with peptides of Notch receptor. Significant conformational changes are required in the ankyrin repeat fold of Notch receptor to enable hydroxylation	Homo sapiens, Mus musculus	-	687592

pH STABILITY	pH STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

TEMPERATURE STABILITY	TEMPERATURE STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

GENERAL STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

ORGANIC SOLVENT	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

OXIDATION STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

STORAGE STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
4�C, 50 mM Tris-HCl pH 7.2, 1 mg/ml bovine serum albumin, at least 8 weeks stable	Bos taurus	-	439258

Purification/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
-	Bos taurus	-	439256
partial	Bos taurus	-	439259
two enzymes, one 52000 Da, the other 56000 Da	Bos taurus	-	439258
gel filtration, above 95% purity	Homo sapiens	-	699589
His6 affinity chromatography	Homo sapiens	-	676817
Ni2+-NTA garose affinity chromatography	Homo sapiens	-	698201
recombinant enzyme	Homo sapiens	-	659316
recombinant enzymes	Homo sapiens	Q9NWT6	659445

Cloned/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
expressed in wild-type Escherichia coli and in Escherichia coli BL21	Bos taurus	-	439256
wild-type and mutant enzymes expressed in Escherichia coli	Bos taurus	-	439253
wild-type and mutants expressed in Escherichia coli BL21	Bos taurus	-	439255
-	Homo sapiens	-	687022
co-immunization in Mus musculus, thereafter cell fusion of immunized mice splenic lymphocytes with myouse myeloma cells; expression in Escherichia coli DH5alpha	Homo sapiens	-	698201
expressed in HEK293T cells and in Escherichia coli	Homo sapiens	-	659316
expressed in Mus musculus liver cells	Homo sapiens	-	675183
expressed in Sf9 insect cells as His-tag and GST-fusion protein	Homo sapiens	Q9NWT6	659445
expression in Escherichia coli BL21	Homo sapiens	-	699589
in vitro transcription and translation in the presence of canine pancreas microsomes	Homo sapiens	-	439257
transfected into NIH-3T3 cells	Homo sapiens	-	439252
expression in human CNS neuronal and hepatocellulcar carcinoma cells	Rattus norvegicus	-	695432

EXPRESSION	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
overexpression in malignant neoplasms	Homo sapiens	-	698195
about 2fold increase in expression in double transgenic mice with expression of hepatitis Bx and insulin receptor substrate-1 under a liver-specific promoter	Mus musculus	-	698158
ethanol-treated cultures	Rattus norvegicus	-	695432
increase after inhibition of GSK-3beta or global caspases	Rattus norvegicus	-	695432

ENGINEERING	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
C637A	Bos taurus	-	38% activity of wild-type	439253
C644A	Bos taurus	-	62% activity of wild-type	439253
C656A	Bos taurus	-	100% activity of wild-type	439253
C681A	Bos taurus	-	60% activity of wild-type	439253
C696A	Bos taurus	-	29% activity of wild-type	439253
G659A	Bos taurus	-	21% activity of wild-type	439255
G669A	Bos taurus	-	90% activity of wild-type	439255
H667L	Bos taurus	-	106% activity of wild-type	439255
H671L	Bos taurus	-	16% activity of wild-type	439255
H675D	Bos taurus	-	20% activity of wild-type	439255
H675E	Bos taurus	-	12% activity of wild-type	439255
H686L	Bos taurus	-	9% activity of wild-type	439255
P678V	Bos taurus	-	90% activity of wild-type	439255
R682A	Bos taurus	-	10% activity of wild-type	439255
R684A	Bos taurus	-	8% activity of wild-type	439255
R684K	Bos taurus	-	87% activity of wild-type	439255

Renatured/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

APPLICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
analysis	Homo sapiens	-	isolation of human single-chain Fv fragments directed against human aspartyl-asparaginyl beta-hydroxylase. Antibodies show significant binding to recombinant enzyme in ELISA, tumor cell lines, and tumor tissues. They target different domains of the enzyme. A goat-anti-human IgG saporin conjugate can be delivered into tumor cells by antibody 6-22 and elicits cytotoxicity toward the tumor cells in vitro	687022
medicine	Homo sapiens	-	insulin-like growth factor-1 stimulates peptide-aspartate beta-dioxygenase protein expression and directional motility. Ethanol reduces the stimulation without inhibition of the mRNA expression, and phosphorylation of the enzyme is higher in ethanol-treated compared with control cells	684228
medicine	Homo sapiens	-	isolation of human single-chain Fv fragments directed against human aspartyl-asparaginyl beta-hydroxylase. Antibodies show significant binding to recombinant enzyme in ELISA, tumor cell lines, and tumor tissues. They target different domains of the enzyme. A goat-anti-human IgG saporin conjugate can be delivered into tumor cells by antibody 6-22 and elicits cytotoxicity toward the tumor cells in vitro	687022
medicine	Homo sapiens	-	biomarker to prognosticate non-small cell lung cancer	698195

DISEASE	TITLE OF PUBLICATION	LINK TO PUBMED
Abortion, Missed	[Expression of aspartyl-(asparaginyl) beta-hydroxylase in villi in patients with missed abortion]	PubMed
Bile Duct Neoplasms	Detection of human aspartyl (asparaginyl) beta-hydroxylase and homeobox B7 mRNA in brush cytology specimens from patients with bile duct cancer.	PubMed
Breast Neoplasms	Cytoplasmic location of factor-inhibiting hypoxia-inducible factor is associated with an enhanced hypoxic response and a shorter survival in invasive breast cancer.	PubMed
Breast Neoplasms	The key hypoxia regulated gene CAIX is upregulated in basal-like breast tumours and is associated with resistance to chemotherapy.	PubMed
CADASIL	Glial Vascular Degeneration in CADASIL.	PubMed
Carcinoma	Aspartyl-(asparaginyl)-beta-hydroxylase regulates hepatocellular carcinoma invasiveness.	PubMed
Carcinoma	DNA methylation analysis of the HIF-1? prolyl hydroxylase domain genes PHD1, PHD2, PHD3 and the factor inhibiting HIF gene FIH in invasive breast carcinomas.	PubMed
Carcinoma	Human aspartyl (asparaginyl) beta-hydroxylase monoclonal antibodies: potential biomarkers for pancreatic carcinoma.	PubMed
Carcinoma	Prognostic value of aspartyl (asparaginyl)-beta-hydroxylase/humbug expression in non-small cell lung carcinoma.	PubMed
Carcinoma	Prognostic value of humbug gene overexpression in stage II colon cancer.	PubMed
Carcinoma, Hepatocellular	Aspartyl-(asparaginyl)-beta-hydroxylase regulates hepatocellular carcinoma invasiveness.	PubMed
Carcinoma, Hepatocellular	Aspartyl-asparagyl beta hydroxylase over-expression in human hepatoma is linked to activation of insulin-like growth factor and notch signaling mechanisms.	PubMed
Carcinoma, Hepatocellular	Expression of aspartyl beta-hydroxylase and its clinicopathological significance in hepatocellular carcinoma.	PubMed
Carcinoma, Hepatocellular	Monoclonal antibodies against human aspartyl (asparaginyl) beta-hydroxylase developed by DNA immunization.	PubMed
Carcinoma, Hepatocellular	Overexpression of aspartyl-(asparaginyl)-beta-hydroxylase in hepatocellular carcinoma is associated with worse surgical outcome.	PubMed
Carcinoma, Hepatocellular	Prognostic value of humbug gene overexpression in stage II colon cancer.	PubMed
Cholangiocarcinoma	Antisense oligodeoxynucleotides directed against aspartyl (asparaginyl) beta-hydroxylase suppress migration of cholangiocarcinoma cells.	PubMed
Cholangiocarcinoma	Clinicopathological correlates of aspartyl (asparaginyl) beta-hydroxylase over-expression in cholangiocarcinoma.	PubMed
Cholangiocarcinoma	Prognostic value of humbug gene overexpression in stage II colon cancer.	PubMed
Kidney Neoplasms	Factor inhibiting HIF (FIH-1) promotes renal cancer cell survival by protecting cells from HIF-1?-mediated apoptosis.	PubMed
Myocardial Infarction	Double knockdown of prolyl hydroxylase and factor-inhibiting hypoxia-inducible factor with nonviral minicircle gene therapy enhances stem cell mobilization and angiogenesis after myocardial infarction.	PubMed
Neoplasm Metastasis	Expression of aspartyl beta-hydroxylase and its clinicopathological significance in hepatocellular carcinoma.	PubMed
Neoplasms	A hypoxic niche regulates glioblastoma stem cells through hypoxia inducible factor 2 alpha.	PubMed
Neoplasms	Antisense oligonucleotides selectively regulate aspartyl beta-hydroxylase and its truncated protein isoform in vitro but distribute poorly into A549 tumors in vivo.	PubMed
Neoplasms	Aspartyl-asparagyl beta hydroxylase over-expression in human hepatoma is linked to activation of insulin-like growth factor and notch signaling mechanisms.	PubMed
Neoplasms	Clinicopathological correlates of aspartyl (asparaginyl) beta-hydroxylase over-expression in cholangiocarcinoma.	PubMed
Neoplasms	Detection of human aspartyl (asparaginyl) beta-hydroxylase and homeobox B7 mRNA in brush cytology specimens from patients with bile duct cancer.	PubMed
Neoplasms	Expression of aspartyl beta-hydroxylase and its clinicopathological significance in hepatocellular carcinoma.	PubMed
Neoplasms	Isolation and characterization of human antibodies targeting human aspartyl (asparaginyl) beta-hydroxylase.	PubMed
Neoplasms	Role of the aspartyl-asparaginyl-beta-hydroxylase gene in neuroblastoma cell motility.	PubMed
Neoplasms	The key hypoxia regulated gene CAIX is upregulated in basal-like breast tumours and is associated with resistance to chemotherapy.	PubMed
Neoplasms	Transcriptional activity and Sp 1/3 transcription factor binding to the P1 promoter sequences of the human AbetaH-J-J locus.	PubMed
Neoplasms	[Expression of human aspartyl beta-hydroxylase and preparation of its monoclonal antibody].	PubMed
Neuroblastoma	Differential growth factor regulation of aspartyl-(asparaginyl)-beta-hydroxylase family genes in SH-Sy5y human neuroblastoma cells.	PubMed
Neuroblastoma	Role of the aspartyl-asparaginyl-beta-hydroxylase gene in neuroblastoma cell motility.	PubMed

REF.	AUTHORS	TITLE	JOURNAL	VOL.	PAGES	YEAR	ORGANISM	LINK TO PUBMED	SOURCE
439251	Dinchuk, J.E.; Henderson, N.L.; Burn, T.C.; Huber, R.; Ho, S.P.; Link, J.; O'Neil, K.T.; Focht, R.J.; Scully, M.S.; Hollis, J.M.; Hollis, G.F.; Friedman, P.A.	Aspartyl beta-hydroxylase (Asph) and an evolutionarily conserved isoform of Asph missing the catalytic domain share exons with junctin	J. Biol. Chem.	275	39543-39554	2000	Mus musculus	PubMed
439252	Ince, N.; De la Monte, S.M.; Wands, J.R.	Overexpression of human aspartyl (asparaginyl) beta-hydroxylase is associated with malignant transformation	Cancer Res.	60	1261-1266	2000	Homo sapiens	PubMed
439253	McGinnis, K.; Ku, G.M.; Fu, J.; Stern, A.M.; Friedman, P.A.	The five cysteine residues located in the active site region of bovine aspartyl (asparaginyl) beta-hydroxylase are not essential for catalysis	Biochim. Biophys. Acta	1387	454-456	1998	Bos taurus	PubMed
439254	Lavaissiere, L.; Jia, S.; Nishiyama, M.; de la Monte, S.; Stern, A.M.; Wands, J.R.; Friedman, P.A.	Overexpression of human aspartyl(asparaginyl)beta-hydroxylase in hepatocellular carcinoma and cholangiocarcinoma	J. Clin. Invest.	98	1313-1323	1996	Homo sapiens	PubMed
439255	McGinnis, K.; Ku, G.M.; VanDusen, W.J.; Fu, J.; Garsky, V.; Stern, A.M.; Friedman, P.A.	Site-directed mutagenesis of residues in a conserved region of bovine aspartyl (asparaginyl) beta-hydroxylase: Evidence that histidine 675 has a role in binding Fe2+	Biochemistry	35	3957-3962	1996	Bos taurus	PubMed
439256	Jia, S.; McGinnis, K.; VanDusen, W.J.; Burke, C.J.; Kuo, A.; Griffin, P.R.; Sardana, M.K.; Elliston, K.O.; Stern, A.M.; Friedman, P.A.	A fully active catalytic domain of bovine aspartyl (asparaginyl) beta-hydroxylase expressed in Escherichia coli: characterization and evidence for the identification of an active-site region in vertebrate alpha-ketoglutarate-dependent dioxygenases	Proc. Natl. Acad. Sci. USA	91	7227-7231	1994	Bos taurus	PubMed
439257	Korioth, F.; Gieffers, C.; Frey, J.	Cloning and characterization of the human gene encoding aspartyl beta-hydroxylase	Gene	150	395-399	1994	Homo sapiens	PubMed
439258	Wang, Q.; VanDusen, W.J.; Petrosky, C.J.; Garsky, V.M.; Stern, A.M.; Friedman, P.A.	Bovine liver aspartyl beta-hydroxylase. Purification and characterization	J. Biol. Chem.	266	14004-14010	1991	Bos taurus	PubMed
439259	Gronke, R.S.; Welsch, D.J.; VanDusen, W.J.; Garsky, V.M.; Sardana, M.K.; Stern, A.M.; Friedman, P.A.	Partial purification and characterization of bovine liver aspartyl beta-hydroxylase	J. Biol. Chem.	265	8558-8565	1990	Bos taurus	PubMed
439260	Gronke, R.S.; VanDusen, W.J.; Garsky, V.M.; Jacobs, J.W.; Sardana, M.K.; Stern, A.M.; Friedman, P.A.	Aspartyl beta-hydroxylase: in vitro hydroxylation of a synthetic peptide based on the structure of the first growth factor-like domain of human factor IX	Proc. Natl. Acad. Sci. USA	86	3609-3613	1989	Mus musculus, Rattus norvegicus	PubMed
657901	Lancaster, D.E.; McDonough, M.A.; Schofield, C.J.	Factor inhibiting hypoxia-inducible factor (FIH) and other asparaginyl hydroxylases	Biochem. Soc. Trans.	32	943-945	2004	Homo sapiens	PubMed
659316	Linke, S.; Stojkoski, C.; Kewley, R.J.; Booker, G.W.; Whitelaw, M.L.; Peet, D.J.	Substrate requirements of the oxygen-sensing asparaginyl hydroxylase factor-inhibiting hypoxia-inducible factor	J. Biol. Chem.	279	14391-14397	2004	Homo sapiens	PubMed
659445	Koivunen, P.; Hirsilae, M.; Guenzler, V.; Kivirikko, K.I.; Myllyharju, J.	Catalytic properties of the asparaginyl hydroxylase (FIH) in the oxygen sensing pathway are distinct from those of its prolyl 4-hydroxylases	J. Biol. Chem.	279	9899-9904	2004	Homo sapiens	PubMed
673915	Cantarini, M.C.; de la Monte, S.M.; Pang, M.; Tong, M.; DErrico, A.; Trevisani, F.; Wands, J.R.	Aspartyl-asparagyl beta hydroxylase over-expression in human hepatoma is linked to activation of insulin-like growth factor and notch signaling mechanisms	Hepatology	44	446-457	2006	Homo sapiens	PubMed
673951	Gundogan, F.; Elwood, G.; Greco, D.; Rubin, L.P.; Pinar, H.; Carlson, R.I.; Wands, J.R.; de la Monte, S.M.	Role of aspartyl-(asparaginyl) beta-hydroxylase in placental implantation: Relevance to early pregnancy loss	Hum. Pathol.	38	50-59	2007	Homo sapiens	PubMed
675183	de la Monte, S.M.; Tamaki, S.; Cantarini, M.C.; Ince, N.; Wiedmann, M.; Carter, J.J.; Lahousse, S.A.; Califano, S.; Maeda, T.; Ueno, T.; DErrico, A.; Trevisani, F.; Wands, J.R.	Aspartyl-(asparaginyl)-beta-hydroxylase regulates hepatocellular carcinoma invasiveness	J. Hepatol.	44	971-983	2006	Homo sapiens	PubMed
675861	Xian, Z.H.; Zhang, S.H.; Cong, W.M.; Yan, H.X.; Wang, K.; Wu, M.C.	Expression of aspartyl beta-hydroxylase and its clinicopathological significance in hepatocellular carcinoma	Mod. Pathol.	19	280-286	2006	Homo sapiens	PubMed
676817	Cockman, M.E.; Lancaster, D.E.; Stolze, I.P.; Hewitson, K.S.; McDonough, M.A.; Coleman, M.L.; Coles, C.H.; Yu, X.; Hay, R.T.; Ley, S.C.; Pugh, C.W.; Oldham, N.J.; Masson, N.; Schofield, C.J.; Ratcliffe, P.J.	Posttranslational hydroxylation of ankyrin repeats in IkB proteins by the hypoxia-inducible factor (HIF) asparaginyl hydroxylase, factor inhibiting HIF (FIH)	Proc. Natl. Acad. Sci. USA	103	14767-14772	2006	Homo sapiens	PubMed
684228	Carter, J.J.; Tong, M.; Silbermann, E.; Lahousse, S.A.; Ding, F.F.; Longato, L.; Roper, N.; Wands, J.R.; de la Monte, S.M.	Ethanol impaired neuronal migration is associated with reduced aspartyl-asparaginyl-beta-hydroxylase expression	Acta Neuropathol.	116	303-315	2008	Homo sapiens	PubMed
687022	Yeung, Y.A.; Finney, A.H.; Koyrakh, I.A.; Lebowitz, M.S.; Ghanbari, H.A.; Wands, J.R.; Wittrup, K.D.	Isolation and characterization of human antibodies targeting human aspartyl (asparaginyl) beta-hydroxylase	Hum. Antibodies	16	163-176	2007	Homo sapiens	PubMed
687592	Coleman, M.L.; McDonough, M.A.; Hewitson, K.S.; Coles, C.; Mecinovic, J.; Edelmann, M.; Cook, K.M.; Cockman, M.E.; Lancaster, D.E.; Kessler, B.M.; Oldham, N.J.; Ratcliffe, P.J.; Schofield, C.J.	Asparaginyl hydroxylation of the Notch ankyrin repeat domain by factor inhibiting hypoxia-inducible factor	J. Biol. Chem.	282	24027-24038	2007	Homo sapiens, Mus musculus	PubMed
695432	de la Monte, S.M.; Tong, M.; Carlson, R.I.; Carter, J.J.; Longato, L.; Silbermann, E.; Wands, J.R.	Ethanol inhibition of aspartyl-asparaginyl-beta-hydroxylase in fetal alcohol spectrum disorder: potential link to the impairments in central nervous system neuronal migration	Alcohol	43	225-240	2009	Rattus norvegicus	PubMed
698158	Longato, L.; de la Monte, S.; Kuzushita, N.; Horimoto, M.; Rogers, A.B.; Slagle, B.L.; Wands, J.R.	Overexpression of insulin receptor substrate-1 and hepatitis Bx genes causes premalignant alterations in the liver	Hepatology	49	1935-1943	2009	Mus musculus	PubMed
698195	Luu, M.; Sabo, E.; de la Monte, S.M.; Greaves, W.; Wang, J.; Tavares, R.; Simao, L.; Wands, J.R.; Resnick, M.B.; Wang, L.	Prognostic value of aspartyl (asparaginyl)-beta-hydroxylase/humbug expression in non-small cell lung carcinoma	Hum. Pathol.	40	639-644	2009	Homo sapiens	PubMed
698201	Xue, T.; Xue, X.P.; Huang, Q.S.; Wei, L.; Sun, K.; Xue, T.	Monoclonal antibodies against human aspartyl (asparaginyl) beta-hydroxylase developed by DNA immunization	Hybridoma	28	251-257	2009	Homo sapiens	PubMed
699589	Hardy, A.P.; Prokes, I.; Kelly, L.; Campbell, I.D.; Schofield, C.J.	Asparaginyl beta-hydroxylation of proteins containing ankyrin repeat domains influences their stability and function	J. Mol. Biol.	392	994-1006	2009	Homo sapiens	PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 1.14.11.16)

NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM

IUBMB Enzyme Nomenclature

PROSITE Database of protein families and domains

SYSTERS

Protein Mutant Database

InterPro (database of protein families, domains and functional sites)

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