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Literature summary extracted from

  • Campbell, T.A.; Zlotnick, G.W.; Neubert, T.A.; Sacci, J.B.; Gottlieb, M.
    Purification and characterization of 3'-nucleotidase/nuclease from promastigotes of Leishmania donovani (1991), Mol. Biochem. Parasitol., 47, 109-117.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.3.6 medicine leishmanial 3'-nucleotidase could become a target for chemotherapeutic attack because an enzyme with similar properties has not been detected in mammalian tissues Leishmania donovani

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.6 EDTA
-
 Leishmania donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.6 0.16
-
 3'-AMP
-
 Leishmania donovani

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.6 plasma membrane surface membrane associated Leishmania donovani 5886
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.6 Leishmania donovani
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.6 side-chain modification glycoprotein Leishmania donovani

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.6
-
 Leishmania donovani

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.6 1827
-
-
 Leishmania donovani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.6 3'-AMP + H2O
-
 Leishmania donovani adenosine + phosphate
-
 ?
3.1.3.6 3'-GMP + H2O
-
 Leishmania donovani guanosine + phosphate
-
 ?
3.1.3.6 3'-UMP + H2O
-
 Leishmania donovani uridine + phosphate
-
 ?
3.1.3.6 adenosine 3',5'-diphosphate + H2O
-
 Leishmania donovani ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.6 3'-N'ase
-
 Leishmania donovani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.6 8.5
-
 the presence of dithiothreitol shifts the optimum to pH 6.0 Leishmania donovani