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Literature summary extracted from

  • Imagawa, H.; Toryu, H.; Ozawa, T.; Takino, Y.
    Purification and characterization of nucleases from tea leaves (1982), Agric. Biol. Chem., 46, 1261-1269.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.6 Co2+
-
 Camellia sinensis
3.1.3.6 Cu2+
-
 Camellia sinensis
3.1.3.6 Fe3+
-
 Camellia sinensis
3.1.3.6 Mg2+
-
 Camellia sinensis
3.1.3.6 Mn2+
-
 Camellia sinensis
3.1.30.2 Co2+
-
 Camellia sinensis
3.1.30.2 Cu2+
-
 Camellia sinensis
3.1.30.2 EDTA
-
 Camellia sinensis
3.1.30.2 Fe3+
-
 Camellia sinensis
3.1.30.2 Mg2+
-
 Camellia sinensis
3.1.30.2 Mn2+ inhibits hydrolysis of RNA, denatured DNA and 3'-AMP, not: native DNA Camellia sinensis
3.1.30.2 p-chloromercuribenzoate
-
 Camellia sinensis
3.1.30.2 Zn2+
-
 Camellia sinensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.30.2 Zn2+ inhibition Camellia sinensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.30.2 33000
-
 form B, gel filtration Camellia sinensis
3.1.30.2 35000
-
 form A, gel filtration Camellia sinensis

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.6 Camellia sinensis
-
 nuclease A and nuclease B, both with 3'-nucleotidase activity
-
3.1.30.2 Camellia sinensis
-
 tea, 2 forms: A, B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.6 nuclease A and nuclease B, both with 3'-nucleotidase activity Camellia sinensis
3.1.30.2 2 forms: A, B Camellia sinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.6 leaf
-
 Camellia sinensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.30.2 additional information
-
-
 Camellia sinensis

Storage Stability

EC Number Storage Stability Organism
3.1.3.6 4°C, 0.04 M sodium acetate buffer, pH 5.5, 4 months stable Camellia sinensis
3.1.30.2 4°C, pH 5.5, 0.04 M sodium acetate buffer, 4 months Camellia sinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.6 3'-AMP + H2O
-
 Camellia sinensis adenosine + phosphate
-
 ?
3.1.3.6 3'-CMP + H2O
-
 Camellia sinensis cytosine + phosphate
-
 ?
3.1.3.6 3'-dAMP + H2O
-
 Camellia sinensis 3'-deoxyadenosine + phosphate
-
 ?
3.1.3.6 3'-GMP + H2O
-
 Camellia sinensis guanosine + phosphate
-
 ?
3.1.3.6 3'-UMP + H2O
-
 Camellia sinensis uridine + phosphate
-
 ?
3.1.30.2 3'-nucleotides + H2O e.g. 3'-AMP Camellia sinensis ?
-
 ?
3.1.30.2 DNA + H2O heat denatured Camellia sinensis 5'-phosphonucleotides + 5'-phosphomononucleotides
-
 ?
3.1.30.2 DNA + H2O native double-stranded Camellia sinensis 5'-phosphonucleotides + 5'-phosphomononucleotides
-
 ?
3.1.30.2 additional information enzyme also shows 3'-nucleotidase activity Camellia sinensis ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.6 60 70 nuclease B with 3'-nucleotidase activity Camellia sinensis
3.1.3.6 70
-
 nuclease A with 3'-nucleotidase activity Camellia sinensis
3.1.30.2 60 70 nuclease B: RNA, denatured DNA, 3'-AMP Camellia sinensis
3.1.30.2 70
-
 nuclease A, RNA, denatured DNA, 3'-AMP Camellia sinensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.30.2 50 80 50°C: 50% nuclease B, 10% nuclease A of activity maximum, 80°C: 30% nuclease B, 60% nuclease A of activity maximum Camellia sinensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.6 60
-
 stable below Camellia sinensis
3.1.3.6 80
-
 15 min, 80% inactivation Camellia sinensis
3.1.30.2 60
-
 below Camellia sinensis
3.1.30.2 80
-
 15 min, 80% loss of activity Camellia sinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.6 6
-
 substrates 3'-GMP, 3'-CMP Camellia sinensis
3.1.3.6 6.5
-
 substrates 3'-UMP, 3'-AMP Camellia sinensis
3.1.30.2 5.5
-
 native DNA Camellia sinensis
3.1.30.2 6
-
 RNA, denatured DNA Camellia sinensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.30.2 additional information
-
-
 Camellia sinensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.6 6 8 nuclease A and nuclease B, both with 3'-nucleotidase activity Camellia sinensis
3.1.30.2 6 8 4°C Camellia sinensis