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Literature summary extracted from

  • Hinderer, W.; Seitz, H.U.
    In vitro inhibition of carrot chalcone synthase by 3'-nucleotidase: the role of the 3-phosphate group of malonyl-coenzyme A in flavonoid biosynthesis (1986), Arch. Biochem. Biophys., 246, 217-224.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.74 3'-Nucleotidase i.e. EC 3.1.3.6 hydrolyzes the phosphate group in the 3'-position of adenosine, a part of the CoA thioester substrates Daucus carota
2.3.1.74 malonyl-3'-dephospho-CoA 50% inhibition at 0.003 mM Daucus carota

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.74 4-coumaroyl-CoA + 3 malonyl-CoA Daucus carota
-
naringenin chalcone + 4 CoA + 3 CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.74 Daucus carota
-
carrot, ssp. sativa
-
3.1.3.6 Daucus carota
-
carrot
-
3.1.3.6 Lolium multiflorum
-
rye grass
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.6
-
Daucus carota

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.74 cell suspension culture
-
Daucus carota
-
3.1.3.6 cell culture
-
Daucus carota
-

Storage Stability

EC Number Storage Stability Organism
3.1.3.6 -70°C Daucus carota

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.74 4-coumaroyl-CoA + 3 malonyl-CoA
-
Daucus carota naringenin chalcone + 4 CoA + 3 CO2
-
?
3.1.3.6 3'-AMP + H2O
-
Daucus carota adenosine + phosphate
-
?
3.1.3.6 3'-AMP + H2O
-
Lolium multiflorum adenosine + phosphate
-
?
3.1.3.6 3'-GMP + H2O 29% of the activity with 3'-AMP Daucus carota guanosine + phosphate
-
?
3.1.3.6 acetyl-CoA + H2O 68% of the activity with 3'-AMP Daucus carota ?
-
?
3.1.3.6 adenosine 3',5'-diphosphate + H2O 83% of the activity with 3'-AMP Daucus carota ?
-
?
3.1.3.6 CoA-SH + H2O 53% of the activity with 3'-AMP Daucus carota ?
-
?
3.1.3.6 malonyl-CoA + H2O 53% of the activity with 3'-AMP Daucus carota ?
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.6 7
-
-
Daucus carota