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Literature summary extracted from

  • Brown, P.H.; Ho, T.D.
    Biochemical properties and hormonal regulation of barley nuclease (1987), Eur. J. Biochem., 168, 357-364.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.30.2 dithiothreitol
-
 Hordeum vulgare
3.1.30.2 EDTA
-
 Hordeum vulgare
3.1.30.2 SDS
-
 Hordeum vulgare

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.30.2 Mg2+ no reactivation after EDTA treatment Hordeum vulgare
3.1.30.2 additional information no reactivation after EDTA treatment by Cd2+, Cu2+ Hordeum vulgare
3.1.30.2 Zn2+ reactivates after EDTA treatment Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.30.2 33000
-
 1 * 33000, decarboxylated, SDS-PAGE Hordeum vulgare
3.1.30.2 35000
-
 immunoprecipitation Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.6 Hordeum vulgare
-
 barley, also acts as a nuclease, hydrolyzing RNA and DNA
-
3.1.30.2 Hordeum vulgare
-
 barley
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.30.2 glycoprotein about 8% carbohydrate, asparagin-linked high-mannose oligosaccharide Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.6 aleurone layer
-
 Hordeum vulgare
-
3.1.30.2 seed
-
 Hordeum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.6 3'-AMP + H2O
-
 Hordeum vulgare adenosine + phosphate
-
 ?
3.1.3.6 3'-GMP + H2O 45% of the activity with 3'-AMP Hordeum vulgare guanosine + phosphate
-
 ?
3.1.30.2 DNA + H2O
-
 Hordeum vulgare 5'-phosphonucleotides + 5'-phosphomononucleotides
-
 ?
3.1.30.2 additional information inactive towards 3'-phosphoester linkage of nucleoside cyclic 2',3'- and 3',5'-monophosphates Hordeum vulgare ?
-
 ?
3.1.30.2 additional information enzyme also shows 3'-nucleotidase activity Hordeum vulgare ?
-
 ?
3.1.30.2 additional information 3'-nucleotidase activity is greater for purine than for pyrimidine ribonucleotides Hordeum vulgare ?
-
 ?
3.1.30.2 additional information little activity towards ribonucleoside 2'- and 5'-monophosphates and deoxyribonucleoside 3'- and 5'-monophosphates Hordeum vulgare ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.30.2 monomer 1 * 33000, decarboxylated, SDS-PAGE Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
3.1.30.2 barley nuclease
-
 Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.6 55
-
-
 Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.6 6 6.5
-
 Hordeum vulgare