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Literature summary extracted from

  • Orford, M.R.; Saggerson, E.D.
    A low-Km 5'-nucleotidase from rat brain cytosolic fraction: purification, kinetic properties, and description of regulation by a novel factor that increases sensitivity to inhibition by ATP and ADP (1996), J. Neurochem., 67, 795-804.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 ADP
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 0.015
-
 5'-AMP
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 cytoplasm comparison of the membrane-bound and cytosolic form, discussion of the origin of the cytosolic form Rattus norvegicus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 54300
-
 4 * 54300, brain cytoplasm, SDS-PAGE Rattus norvegicus
3.1.3.5 230000
-
 brain cytoplasm, gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5 brain cytosol Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.5 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.5 15.6
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O
-
 Rattus norvegicus adenosine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O 65% of the activity with 5'-AMP, brain cytosolic enzyme Rattus norvegicus cytidine + phosphate
-
 ?
3.1.3.5 5'-dAMP + H2O 7% of the activity with 5'-AMP, brain cytosolic enzyme Rattus norvegicus deoxyadenosine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O 19% of the activity with 5'-AMP Rattus norvegicus guanosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O 36% of the activity with 5'-AMP, brain cytosolic enzyme Rattus norvegicus inosine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O 111% of the activity with 5'-AMP, brain cytosolic enzyme Rattus norvegicus uridine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.5 tetramer 4 * 54300, brain cytoplasm, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.5 5'Nase
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.5 7 9 brain, cytosolic enzyme Rattus norvegicus