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Literature summary extracted from

  • Lee, R.S.F.; Ford, H.C.
    Purification and characterization of 5'-nucleotidase/FAD pyrophosphatase from human placenta (1997), Methods Enzymol., 280, 424-436.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 EDTA
-
 Homo sapiens
3.6.1.18 EDTA strong inhibition Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 microvillus
-
 Homo sapiens 5902
-
3.6.1.18 microvillus membrane bound Homo sapiens 5902
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.18 Co2+ partially reverses EDTA inhibition, activating Homo sapiens
3.6.1.18 Mn2+ restores activity after EDTA inhibition, activating Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.18 44000
-
 more than one enzyme suggested Homo sapiens
3.6.1.18 70000
-
 more than one enzyme suggested Homo sapiens
3.6.1.18 74000
-
 more than one enzyme suggested Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.18 FAD + H2O Homo sapiens
-
 FMN + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Homo sapiens
-
-
-
3.6.1.18 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.5 side-chain modification glycoprotein Homo sapiens
3.6.1.18 glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5
-
 Homo sapiens
3.6.1.18
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.5 placenta
-
 Homo sapiens
-
3.6.1.18 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.5 94
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O
-
 Homo sapiens adenosine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O
-
 Homo sapiens cytidine + phosphate
-
 ?
3.1.3.5 5'-dCMP + H2O i.e. 2'-deoxycytosine 5'-phosphate Homo sapiens deoxycytidine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O
-
 Homo sapiens guanosine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O
-
 Homo sapiens uridine + phosphate
-
 ?
3.6.1.18 FAD + H2O
-
 Homo sapiens FMN + AMP
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.5 56
-
 30 min, 44% loss of activity Homo sapiens
3.6.1.18 56
-
 44% loss of activity within 30 min Homo sapiens