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Literature summary extracted from

  • Dornand, J.; Bonnafous, J.C.; Mani, J.C.
    Purification and properties of 5'-nucleotidase from lymphocyte plasma membranes (1978), Eur. J. Biochem., 87, 459-465.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 ATP
-
 Sus scrofa
3.1.3.5 concanavalin A
-
 Sus scrofa
3.1.3.5 EDTA
-
 Sus scrofa
3.1.3.5 EGTA
-
 Sus scrofa
3.1.3.5 Hg2+
-
 Sus scrofa
3.1.3.5 Ni2+
-
 Sus scrofa
3.1.3.5 Zn2+
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 0.055
-
 5'-AMP
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 plasma membrane the active site is localized on the outer membrane surface Sus scrofa 5886
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 130000
-
 gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.5 side-chain modification glycoprotein Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.5 lymphocyte mesenteric lymph nodes Sus scrofa
-

Storage Stability

EC Number Storage Stability Organism
3.1.3.5 4°C, 10 mM Tris-HCl, pH 7.5, 0.6% deoxycholate, 1 week, stable Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O
-
 Sus scrofa adenosine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O
-
 Sus scrofa cytidine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O
-
 Sus scrofa guanosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O
-
 Sus scrofa inosine + phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.5 7.5
-
-
 Sus scrofa