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Literature summary extracted from

  • Onishi, H.; Kobayashi, T.; Kamekura, M.
    Purification and some properties of an extracellular halophilic 5'-nucleotidase from a moderate halophile, Micrococcus varians subsp. Halophilus (1984), FEMS Microbiol. Lett., 24, 303-306.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 extracellular
-
 Kocuria varians
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.5 KCl 2.5 M NaCl or KCl required Kocuria varians
3.1.3.5 NaCl 2.5 M NaCl or KCl required Kocuria varians

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Kocuria varians
-
 subsp. halophilus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5
-
 Kocuria varians

Storage Stability

EC Number Storage Stability Organism
3.1.3.5 5°C, 2.5 M (NH4)2SO4, 3 months, 50% loss of activity Kocuria varians

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O
-
 Kocuria varians adenosine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O 56.2% of the activity with 5'-AMP Kocuria varians cytidine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O 53.6% of the activity with 5'-AMP Kocuria varians guanosine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O 33.3% of the activity with 5'-AMP Kocuria varians uridine + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.5 35
-
-
 Kocuria varians

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.5 8
-
-
 Kocuria varians