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Literature summary extracted from

  • Yamamoto, H.; Tomioka, K.; Kawai, H.; Endo, K.
    Purification and properties of 5'-nucleotidase from cod muscle (1986), Agric. Biol. Chem., 50, 1123-1129.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 ADP
-
 Gadus macrocephalus
3.1.3.5 ATP
-
 Gadus macrocephalus
3.1.3.5 Cu2+
-
 Gadus macrocephalus
3.1.3.5 EDTA
-
 Gadus macrocephalus
3.1.3.5 Zn2+
-
 Gadus macrocephalus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 0.0067
-
 5'-AMP
-
 Gadus macrocephalus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.5 Mn2+ activation Gadus macrocephalus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 67000
-
 4 * 67000, SDS-PAGE Gadus macrocephalus
3.1.3.5 270000
-
 gel filtration Gadus macrocephalus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Gadus macrocephalus
-
 cod
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5
-
 Gadus macrocephalus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.5 muscle
-
 Gadus macrocephalus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.5 828
-
-
 Gadus macrocephalus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O 101% of the activity of 5'-IMP Gadus macrocephalus adenosine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O 85% of the activity of 5'-IMP Gadus macrocephalus cytidine + phosphate
-
 ?
3.1.3.5 5'-dAMP + H2O i.e. 2'-deoxyadenosine 5'-phosphate Gadus macrocephalus deoxyadenosine + phosphate
-
 ?
3.1.3.5 5'-dAMP + H2O 53% of the activity of 5'-IMP Gadus macrocephalus deoxyadenosine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O 52% of the activity of 5'-IMP Gadus macrocephalus guanosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O equally reactive as 5'-AMP Gadus macrocephalus inosine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O 120% of the activity of 5'-IMP Gadus macrocephalus uridine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.5 tetramer 4 * 67000, SDS-PAGE Gadus macrocephalus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.5 7.6
-
-
 Gadus macrocephalus