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Literature summary extracted from

  • Singh, A.; Singh, R.
    Properties of 5'-nucleotidase from nodules of pigeonpea (Cajanus cajan) (1986), Phytochemistry, 25, 2267-2270.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 adenosine strong inhibition, enzymes N-I and N-II Cajanus cajan
3.1.3.5 Fe3+ enzyme N-I and N-II Cajanus cajan
3.1.3.5 Inosine strong inhibition, enzymes N-I and N-II Cajanus cajan

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 0.12
-
 5'-AMP enzyme N-II Cajanus cajan
3.1.3.5 0.18
-
 5'-AMP enzyme N-I Cajanus cajan
3.1.3.5 0.18
-
 5'-IMP enzyme N-I Cajanus cajan
3.1.3.5 0.2
-
 5'-IMP enzyme N-II Cajanus cajan
3.1.3.5 0.4
-
 5'-GMP enzyme N-I, enzyme N-II Cajanus cajan
3.1.3.5 0.4
-
 5'-CMP enzyme N-I, enzyme N-II Cajanus cajan
3.1.3.5 0.77
-
 5'-XMP enzyme N-I Cajanus cajan
3.1.3.5 0.99
-
 5'-XMP enzyme N-II Cajanus cajan

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 52000
-
 enzyme N-I, gel filtration Cajanus cajan
3.1.3.5 119000
-
 enzyme N-II, gel filtration Cajanus cajan

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Cajanus cajan
-
 pigeonpea, enzymes N-I and N-II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5 enzyme N-I and N-II Cajanus cajan

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.5 nodule
-
 Cajanus cajan
-

Storage Stability

EC Number Storage Stability Organism
3.1.3.5 4°C, 2 weeks stable Cajanus cajan

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O
-
 Cajanus cajan adenosine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O
-
 Cajanus cajan cytidine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O
-
 Cajanus cajan guanosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O
-
 Cajanus cajan inosine + phosphate
-
 ?
3.1.3.5 5'-XMP + H2O
-
 Cajanus cajan xanthosine + phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.5 5.2 5.7 enzymes N-I and N-II Cajanus cajan