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Literature summary extracted from

  • Lian, W.; Wu, D.; Konings, W.N.; Mierau, I.; Hersh, L.B.
    Heterologous expression and characterization of recombinant Lactococcus lactis neutral endopeptidase (neprilysin) (1996), Arch. Biochem. Biophys., 333, 121-126.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.11 cloned into the pQE expression vector, resulting in the fusion of a hexahistidine at the N-terminus, expression in Escherichia coli Lactococcus lactis

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.11 E535Q inactive enzyme Lactococcus lactis
3.4.24.11 H587Q inactive enzyme Lactococcus lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.11 diethyl dicarbonate
-
 Lactococcus lactis
3.4.24.11 phosphoramidon
-
 Lactococcus lactis
3.4.24.11 thiorphan weak Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.11 0.0004
-
 angiotensin I
-
 Lactococcus lactis
3.4.24.11 0.0018
-
 Vasoactive intestinal peptide
-
 Lactococcus lactis
3.4.24.11 0.0028
-
 adrenocorticotropic hormone
-
 Lactococcus lactis
3.4.24.11 0.005
-
 bradykinin
-
 Lactococcus lactis
3.4.24.11 0.00654
-
 beta-endorphin
-
 Lactococcus lactis
3.4.24.11 0.0082
-
 dynorphin A-17
-
 Lactococcus lactis
3.4.24.11 0.01
-
 Substance P
-
 Lactococcus lactis
3.4.24.11 0.01
-
 dynorphin A-13
-
 Lactococcus lactis
3.4.24.11 0.025
-
 dynorphin A-6
-
 Lactococcus lactis
3.4.24.11 0.035
-
 dynorphin A-10
-
 Lactococcus lactis
3.4.24.11 0.04
-
 dynorphin A-9
-
 Lactococcus lactis
3.4.24.11 0.1
-
 Met-enkephalin
-
 Lactococcus lactis
3.4.24.11 0.135
-
 alpha-neoendorphin
-
 Lactococcus lactis
3.4.24.11 0.2
-
 Leu-enkephalin
-
 Lactococcus lactis
3.4.24.11 0.298
-
 dynorphin A-8
-
 Lactococcus lactis
3.4.24.11 0.41
-
 Met-enkephalin amide
-
 Lactococcus lactis
3.4.24.11 0.612
-
 Leu-enkephalinamide
-
 Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.11 Lactococcus lactis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.11
-
 Lactococcus lactis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.11 0.203
-
 cleavage of succinyl-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-4-methylcoumarin 7-amide Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.11 adrenocorticotropic hormone + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 alpha-neoendorphin + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 angiotensin I + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 beta-endorphin + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 beta-neoendorphin + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 bradykinin + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 dynorphin + H2O dynorphin A-6, A-8, A-9, A-10, A-13 and A-17 Lactococcus lactis ?
-
 ?
3.4.24.11 dynorphin A-10 + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 dynorphin A-13 + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 dynorphin A-17 + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 dynorphin A-6 + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 dynorphin A-8 + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 dynorphin A-9 + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 enkephalin + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 Leu-enkephalin + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 Leu5-enkephalinamide + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 Met-enkephalin + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 Met-enkephalin amide + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 Substance P + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 succinyl-Arg-Pro-Phe-His-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
-
 Lactococcus lactis ?
-
 ?
3.4.24.11 Vasoactive intestinal peptide + H2O
-
 Lactococcus lactis ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.11 0.5
-
 Met-enkephalin amide
-
 Lactococcus lactis
3.4.24.11 1.7
-
 adrenocorticotropic hormone
-
 Lactococcus lactis
3.4.24.11 1.8
-
 Vasoactive intestinal peptide
-
 Lactococcus lactis
3.4.24.11 4.3
-
 bradykinin
-
 Lactococcus lactis
3.4.24.11 7.8
-
 Met-enkephalin
-
 Lactococcus lactis
3.4.24.11 8.2
-
 Leu-enkephalinamide
-
 Lactococcus lactis
3.4.24.11 14
-
 dynorphin A-6
-
 Lactococcus lactis
3.4.24.11 15
-
 Leu-enkephalin
-
 Lactococcus lactis
3.4.24.11 18.6
-
 dynorphin A-13
-
 Lactococcus lactis
3.4.24.11 19.6
-
 dynorphin A-8
-
 Lactococcus lactis
3.4.24.11 23.8
-
 dynorphin A-10
-
 Lactococcus lactis
3.4.24.11 25.4
-
 dynorphin A-17
-
 Lactococcus lactis
3.4.24.11 26
-
 beta-endorphin
-
 Lactococcus lactis
3.4.24.11 28
-
 Substance P
-
 Lactococcus lactis
3.4.24.11 32.2
-
 dynorphin A-9
-
 Lactococcus lactis
3.4.24.11 44.6
-
 alpha-neoendorphin
-
 Lactococcus lactis