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Literature summary extracted from

  • Datta, S.; Annapure, U.; Timson, D.
    Characterization of Cd36-03230p, a putative vanillin dehydrogenase from Candida dubliniensis (2016), RSC Adv., 6, 99774-99780 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.67 gene CD36-03230, recombinant expression in Escherichia coli Candida dubliniensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.67 additional information
-
 additional information the enzyme shows positive cooperativity and substrate inhibition with some substrates. With benzaldehyde activity is observed, but the kinetic data cannot be fitted to Michaelis-Menten, cooperative or substrate inhibition kinetic models Candida dubliniensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.67 210000
-
 crosslinking and gel filtration Candida dubliniensis

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.67 Candida dubliniensis B9W7C4
-
-
1.2.1.67 Candida dubliniensis CD36 B9W7C4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.67 4-hydroxybenzaldehyde + NAD+ + H2O
-
 Candida dubliniensis 4-hydroxybenzoate + NADH + 2 H+
-
 ?
1.2.1.67 4-hydroxybenzaldehyde + NAD+ + H2O
-
 Candida dubliniensis CD36 4-hydroxybenzoate + NADH + 2 H+
-
 ?
1.2.1.67 benzaldehyde + NAD+ + H2O
-
 Candida dubliniensis benzoate + NADH + 2 H+
-
 ?
1.2.1.67 benzaldehyde + NAD+ + H2O
-
 Candida dubliniensis CD36 benzoate + NADH + 2 H+
-
 ?
1.2.1.67 cyclohexanecarboxyaldehyde + NAD+ + H2O
-
 Candida dubliniensis benzoate + NADH + 2 H+
-
 ?
1.2.1.67 cyclohexanecarboxyaldehyde + NAD+ + H2O
-
 Candida dubliniensis CD36 benzoate + NADH + 2 H+
-
 ?
1.2.1.67 additional information the enzyme has no detectable activity with vanillin suggesting that the annotation is incorrect. Molecular modeling of CD36-03230p demonstrates that it has an isoleucine residue (Ile156) at key position in the active site, instead of e.g. Met, further strengthening this hypothesis Candida dubliniensis ?
-
 ?
1.2.1.67 additional information the enzyme has no detectable activity with vanillin suggesting that the annotation is incorrect. Molecular modeling of CD36-03230p demonstrates that it has an isoleucine residue (Ile156) at key position in the active site, instead of e.g. Met, further strengthening this hypothesis Candida dubliniensis CD36 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.67 tetramer crosslinking and gel filtration Candida dubliniensis

Synonyms

EC Number Synonyms Comment Organism
1.2.1.67 CD36-03230
-
 Candida dubliniensis
1.2.1.67 Cd36-03230p
-
 Candida dubliniensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.67 30
-
 assay at Candida dubliniensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.67 additional information
-
 the redox cofactor NADP+ and substrates increase the thermal stability of the protein, thermal stabilities (Tm) of Cd36_03230p in the presence of various potential substrates, overview Candida dubliniensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.67 7.3
-
 assay at Candida dubliniensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.67 additional information structure-based multiple sequence alignment shows conserved residues at both NAD(P)+ binding and catalytic domains Candida dubliniensis
1.2.1.67 NAD+
-
 Candida dubliniensis
1.2.1.67 NADP+
-
 Candida dubliniensis

General Information

EC Number General Information Comment Organism
1.2.1.67 additional information enzyme molecular modeling, dimeric model construction using the structure of ALDH domains of Geobacter sulfurreducens PutA, PDB ID 4NMB, for the enzyme dimer and sheep liver class 1 aldehyde dehydrogenase structure, 1BXS, as the template fo rthe tetramer. molecularmodelling of Cd36_03230p predicts that it has a similar fold to other aldehyde dehydrogenases Candida dubliniensis