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Literature summary extracted from
- Heterologous expression and biochemical characterization of two lipoxygenases in oriental melon, Cucumis melo var. makuwa Makino (2016), PLoS ONE, 11, e0153801 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.13.11.12 | gene CmLOX10, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, recombinant expression of His-tagged full-length enzyme in Escherichia coli strain BL21(DE3) | Cucumis melo var. makuwa |
| 1.13.11.12 | gene CmLOX13, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, recombinant expression of His-tagged truncated enzyme in Escherichia coli strain BL21(DE3) | Cucumis melo var. makuwa |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.12 | 0.1258 | - |
linoleic acid | pH 5.0, 45°C, recombinant Hi-tagged enzyme | Cucumis melo var. makuwa |  |
| 1.13.11.12 | 0.1402 | - |
linolenic acid | pH 5.0, 45°C, recombinant Hi-tagged enzyme | Cucumis melo var. makuwa | |
| 1.13.11.12 | 0.1566 | - |
linolenic acid | pH 5.0, 45°C, recombinant Hi-tagged enzyme | Cucumis melo var. makuwa | |
| 1.13.11.12 | 0.2303 | - |
linoleic acid | pH 5.0, 45°C, recombinant Hi-tagged enzyme | Cucumis melo var. makuwa | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.13.11.12 | chloroplast | - |
Cucumis melo var. makuwa | 9507 | - |
| 1.13.11.12 | plasma membrane | - |
Cucumis melo var. makuwa | 5886 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.12 | linoleic acid + O2 | Cucumis melo var. makuwa | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate | - |
? | |
| 1.13.11.12 | linolenic acid + O2 | Cucumis melo var. makuwa | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.12 | Cucumis melo var. makuwa | A0A125S6K7 | cultivar Yumeiren | - |
| 1.13.11.12 | Cucumis melo var. makuwa | A0A125S6K8 | cultivar Yumeiren | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.12 | recombinant His-tagged full-length enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Cucumis melo var. makuwa |
| 1.13.11.12 | recombinant His-tagged truncated enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Cucumis melo var. makuwa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.12 | linoleic acid + O2 | - |
Cucumis melo var. makuwa | (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate | - |
? | |
| 1.13.11.12 | linolenic acid + O2 | - |
Cucumis melo var. makuwa | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.11.12 | ? | x * 102301, sequence calculation | Cucumis melo var. makuwa |
| 1.13.11.12 | ? | x * 102312, sequence calculation | Cucumis melo var. makuwa |
| 1.13.11.12 | More | enzyme domain structure, overview | Cucumis melo var. makuwa |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.11.12 | CmLOX10 | - |
Cucumis melo var. makuwa |
| 1.13.11.12 | CmLOX13 | - |
Cucumis melo var. makuwa |
| 1.13.11.12 | LOX | - |
Cucumis melo var. makuwa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.12 | 35 | - |
- |
Cucumis melo var. makuwa |
| 1.13.11.12 | 45 | - |
- |
Cucumis melo var. makuwa |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.12 | 20 | 50 | enzyme activity of CmLOX10 increases as temperature rises between 20°C and 45°C and then decreases at 50°C, profile overview | Cucumis melo var. makuwa |
| 1.13.11.12 | 20 | 50 | enzyme activity of CmLOX13 is sharply decreased when the temperature is higher than 35°C, profile overview | Cucumis melo var. makuwa |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.12 | 1841.7 | - |
linolenic acid | pH 5.0, 45°C, recombinant Hi-tagged enzyme | Cucumis melo var. makuwa | |
| 1.13.11.12 | 2438.6 | - |
linoleic acid | pH 5.0, 45°C, recombinant Hi-tagged enzyme | Cucumis melo var. makuwa | |
| 1.13.11.12 | 2442.96 | - |
linolenic acid | pH 5.0, 45°C, recombinant Hi-tagged enzyme | Cucumis melo var. makuwa | |
| 1.13.11.12 | 5492.88 | - |
linoleic acid | pH 5.0, 45°C, recombinant Hi-tagged enzyme | Cucumis melo var. makuwa | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.12 | 5 | - |
- |
Cucumis melo var. makuwa |
| 1.13.11.12 | 5.5 | - |
- |
Cucumis melo var. makuwa |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.13.11.12 | Cucumis melo var. makuwa | sequence calculation | - |
6.1 |
| 1.13.11.12 | Cucumis melo var. makuwa | sequence calculation | - |
6.34 |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.13.11.12 | evolution | genes CmLOX10 and CmLOX13 include all of the typical LOX domains and share 58.11% identity at the amino acid level with each other. The phylogenetic analysis reveals that CmLOX10 and CmLOX13 are members of the type 2 13-LOX subgroup which are known to be involved in biotic and abiotic stress. The two lipoxygenases may play different functions in oriental melon during plant growth and development, CmLOX10 and CmLOX13 enzyme reaction shows that both enzymes produce 13S-hydroperoxides when linoleic acid is used as substrate | Cucumis melo var. makuwa |
| 1.13.11.12 | physiological function | the isozyme CmLOX10 is involved in biotic and abiotic stress | Cucumis melo var. makuwa |
| 1.13.11.12 | physiological function | the isozyme CmLOX13 is involved in biotic and abiotic stress | Cucumis melo var. makuwa |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.12 | 11760 | - |
linolenic acid | pH 5.0, 45°C, recombinant Hi-tagged enzyme | Cucumis melo var. makuwa | |
| 1.13.11.12 | 17420 | - |
linolenic acid | pH 5.0, 45°C, recombinant Hi-tagged enzyme | Cucumis melo var. makuwa | |
| 1.13.11.12 | 19390 | - |
linoleic acid | pH 5.0, 45°C, recombinant Hi-tagged enzyme | Cucumis melo var. makuwa | |
| 1.13.11.12 | 23850 | - |
linoleic acid | pH 5.0, 45°C, recombinant Hi-tagged enzyme | Cucumis melo var. makuwa | |
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