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Literature summary extracted from
- Identification and characterization of phenol hydroxylase from phenol-degrading Candida tropicalis strain JH8 (2014), Can. J. Microbiol., 60, 585-591 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.13.7 | expressed in Escherichia coli | Candida tropicalis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.7 | 0.21 | - |
phenol | at pH 7.6 and 30°C | Candida tropicalis |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.7 | 320000 | - |
gel filtration | Candida tropicalis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.7 | phenol + NADPH + H+ + O2 | Candida tropicalis | - |
catechol + NADP+ + H2O | - |
? | |
| 1.14.13.7 | phenol + NADPH + H+ + O2 | Candida tropicalis JH8 | - |
catechol + NADP+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.7 | Candida tropicalis | - |
- |
- |
| 1.14.13.7 | Candida tropicalis JH8 | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.7 | phenol + NADPH + H+ + O2 | - |
Candida tropicalis | catechol + NADP+ + H2O | - |
? | |
| 1.14.13.7 | phenol + NADPH + H+ + O2 | - |
Candida tropicalis JH8 | catechol + NADP+ + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.7 | homotetramer | 4 * 80000, SDS-PAGE | Candida tropicalis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.7 | phenol hydroxylase | - |
Candida tropicalis |
| 1.14.13.7 | phhY | - |
Candida tropicalis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.7 | 30 | - |
- |
Candida tropicalis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.7 | 60 | - |
activity is rapidly lost at temperatures above 60°C | Candida tropicalis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.7 | 7.6 | - |
- |
Candida tropicalis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.7 | NADPH | - |
Candida tropicalis | |
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Release 2023.1 (January 2023)
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