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Literature summary extracted from

  • Eady, R.; Jarman, T.; Large, P.
    Microbial oxidation of amines. Partial purification of a mixed-function secondary-amine oxidase system from Pseudomonas aminovorans that contains an enzymically active cytochrome-P-420-type haemoprotein (1971), Biochem. J., 125, 449-459 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.238 Dimethylamine substrate inhibition at concentrations higher than 1 mM Aminobacter aminovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.238 0.0065
-
 NADH at pH 6.8 and 28°C Aminobacter aminovorans
1.14.13.238 0.0132
-
 NADPH at pH 6.8 and 28°C Aminobacter aminovorans
1.14.13.238 0.041
-
 ethylamine at pH 6.8 and 28°C Aminobacter aminovorans
1.14.13.238 0.058
-
 Dimethylamine at pH 6.8 and 28°C Aminobacter aminovorans
1.14.13.238 0.345
-
 Diethylamine at pH 6.8 and 28°C Aminobacter aminovorans
1.14.13.238 0.63
-
 methyl-n-propylamine at pH 6.8 and 28°C Aminobacter aminovorans
1.14.13.238 0.75
-
 N-Methylethanolamine at pH 6.8 and 28°C Aminobacter aminovorans
1.14.13.238 4.16
-
 ethyl-n-propylamine at pH 6.8 and 28°C Aminobacter aminovorans
1.14.13.238 5.4
-
 n-butylmethylamine at pH 6.8 and 28°C Aminobacter aminovorans

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.238 Aminobacter aminovorans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.238 ammonium sulfate precipitation and DEAE-cellulose column chromatography Aminobacter aminovorans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.238 culture condition:ethylamine-grown cell
-
 Aminobacter aminovorans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.238 diethylamine + NADH + H+ + O2 40% activity compared to dimethylamine Aminobacter aminovorans ethylamine + acetaldehyde + NAD+ + H2O
-
 ?
1.14.13.238 dimethylamine + NADH + H+ + O2 100% activity Aminobacter aminovorans methylamine + formaldehyde + NAD+ + H2O
-
 ?
1.14.13.238 dimethylamine + NADPH + H+ + O2 good substrate Aminobacter aminovorans methylamine + formaldehyde + NADP+ + H2O
-
 ?
1.14.13.238 ethyl-n-propylamine + NADH + H+ + O2 22% activity compared to dimethylamine Aminobacter aminovorans ?
-
 ?
1.14.13.238 ethylamine + NADH + H+ + O2 65% activity compared to dimethylamine Aminobacter aminovorans ?
-
 ?
1.14.13.238 methyl-n-propylamine + NADH + H+ + O2 87% activity compared to dimethylamine Aminobacter aminovorans ?
-
 ?
1.14.13.238 additional information no activity with methylamine, ethylamine, n-propylamine, ethanolamine, aniline, 1,2-diaminopropane, 1,3-diaminopropane, di-isopropylamine, di-n-propylamine, di-isobutylamine, di-n-butylamine, diethanolamine, methyl-n-octylamine, N-methylaniline, benzylmethylamine, putrescine, spermidine, piperidine, sarcosine, N-methylglutamate, noradrenaline, trimethylamine, triethylamine, N,N-dimethylaniline, tetramethylammonium chloride, and tetraethylammonium chloride Aminobacter aminovorans ?
-
 ?
1.14.13.238 n-butylmethylamine + NADH + H+ + O2 54% activity compared to dimethylamine Aminobacter aminovorans ?
-
 ?
1.14.13.238 n-methylethanolamine + NADH + H+ + O2 19% activity compared to dimethylamine Aminobacter aminovorans ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.238 7
-
-
 Aminobacter aminovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.238 NADH
-
 Aminobacter aminovorans
1.14.13.238 NADPH
-
 Aminobacter aminovorans