EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.3.1 | - |
Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.3.1 | F1014I | amino acid exchange in the active site | Mus musculus |
1.2.3.1 | F1014L | amino acid exchange in the active site, 10fold increase in molybdenim content | Mus musculus |
1.2.3.1 | F1014V | amino acid exchange in the active site | Mus musculus |
1.2.3.1 | F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L | all residues in the first coordination sphere around the substrate are exchanged to their counterparts in bovine xanthine oxidoreductase. Mutant shows activity towards benzaldehyde, phthalazine and hypoxanthine | Mus musculus |
1.2.3.1 | F776K/A807E/D878L/L881S/Y885R/P1015T/Y1019L | all residues in the first coordination sphere around the substrate except K889 are exchanged to their counterparts in bovine xanthine oxidoreductase, mutant is devoid of activity towards most substrates tested, while allopurinnol is oxidized at a low rate | Mus musculus |
1.2.3.1 | additional information | exchange of several residues in the active site to the ones found in other Aox homologues in mouse or to residues present in bovine xanthine oxidoreductase. Conversion of Aox3 to an xanthine oxidoreductase is achieved exchanging eight residues in the active site. Exchange of the iron-sulfur clusters FeSI, FeSII and both FeSI/FeSII by the corresponding domains of isoform Aox1 results in a decrease in catalytic activity with all substrates tested and both electron acceptor dichlorophenol indophenol and O2 | Mus musculus |
1.2.3.1 | P1015A | amino acid exchange in the active site | Mus musculus |
1.2.3.1 | P1015G | amino acid exchange in the active site | Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.3.1 | 0.0014 | - |
Phthalazine | wild-type, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0022 | - |
benzaldehyde | mutant P1015A, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0025 | - |
benzaldehyde | wild-type, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0026 | - |
Phthalazine | mutant P1015A, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0033 | - |
benzaldehyde | mutant P1015G, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.009 | - |
Phthalazine | mutant F1014I, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0103 | - |
Phthalazine | mutant P1015G, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0125 | - |
Phthalazine | mutant F1014V, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0215 | - |
benzaldehyde | mutant F1014V, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0227 | - |
benzaldehyde | mutant F1014I, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.032 | - |
phenanthridine | wild-type, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.047 | - |
phenanthridine | mutant P1015G, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.073 | - |
phenanthridine | mutant F1014I, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.087 | - |
N1-methyl-nicotineamide | mutant P1015A, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.089 | - |
N1-methyl-nicotineamide | mutant P1015G, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.099 | - |
phenanthridine | mutant P1015A, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.128 | - |
N1-methyl-nicotineamide | wild-type, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.133 | - |
allopurinol | mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.385 | - |
phenanthridine | mutant F1014V, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.426 | - |
allopurinol | mutant P1015G, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.482 | - |
N1-methyl-nicotineamide | mutant F1014V, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.527 | - |
allopurinol | mutant P1015A, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.733 | - |
Phthalazine | mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 1.595 | - |
allopurinol | wild-type, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 2.21 | - |
benzaldehyde | mutant F776K/A807E/D878L/L881S/Y885R/P1015T/Y1019L, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 2.93 | - |
allopurinol | mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 3.86 | - |
allopurinol | mutant F1014V, pH 8.0,37°C | Mus musculus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.3.1 | Molybdenum | - |
Mus musculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.3.1 | Mus musculus | G3X982 | isoform Aox3 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.3.1 | allopurinol + H2O + 2,6-dichlorophenolindophenol | - |
Mus musculus | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.2.3.1 | benzaldehyde + H2O + 2,6-dichlorophenolindophenol | - |
Mus musculus | benzoate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.2.3.1 | N1-methyl-nicotineamide + H2O + 2,6-dichlorophenolindophenol | - |
Mus musculus | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.2.3.1 | phenanthridine + H2O + O2 | - |
Mus musculus | 6-phenanthridone + H2O2 | - |
? | |
1.2.3.1 | phthalazine + H2O + 2,6-dichlorophenol indophenol | - |
Mus musculus | ? + reduced 2,6-dichlorophenolindophenol | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.3.1 | AOX3 | - |
Mus musculus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.3.1 | 0.18 | - |
phenanthridine | mutant F1014I, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.27 | - |
allopurinol | mutant F1014V, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.31 | - |
benzaldehyde | mutant F1014I, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.38 | - |
N1-methyl-nicotineamide | mutant F1014V, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.4 | - |
N1-methyl-nicotineamide | wild-type, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.62 | - |
allopurinol | mutant P1015G, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.77 | - |
allopurinol | mutant P1015A, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.97 | - |
Phthalazine | mutant F1014I, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 1.08 | - |
N1-methyl-nicotineamide | mutant P1015A, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 1.1 | - |
Phthalazine | wild-type, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 1.13 | - |
benzaldehyde | wild-type, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 1.22 | - |
allopurinol | mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 1.22 | - |
allopurinol | wild-type, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 1.27 | - |
benzaldehyde | mutant P1015A, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 1.42 | - |
phenanthridine | mutant F1014V, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 1.42 | - |
phenanthridine | wild-type, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 1.55 | - |
benzaldehyde | mutant P1015G, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 1.58 | - |
allopurinol | mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 1.92 | - |
N1-methyl-nicotineamide | mutant P1015G, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 2.13 | - |
benzaldehyde | mutant F1014V, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 2.98 | - |
Phthalazine | mutant P1015A, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 3.9 | - |
benzaldehyde | mutant F776K/A807E/D878L/L881S/Y885R/P1015T/Y1019L, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 4.63 | - |
Phthalazine | mutant F1014V, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 5.78 | - |
Phthalazine | mutant P1015G, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 8.73 | - |
Phthalazine | mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 8.83 | - |
phenanthridine | mutant P1015G, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 9.68 | - |
phenanthridine | mutant P1015A, pH 8.0,37°C | Mus musculus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.3.1 | [2Fe-2S]-center | contains two [2Fe2S] clusters | Mus musculus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.3.1 | 0.00008 | - |
allopurinol | mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0001 | - |
allopurinol | mutant F1014V, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0007 | - |
allopurinol | wild-type, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0008 | - |
N1-methyl-nicotineamide | mutant F1014V, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0015 | - |
allopurinol | mutant P1015A, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0015 | - |
allopurinol | mutant P1015G, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0018 | - |
benzaldehyde | mutant F776K/A807E/D878L/L881S/Y885R/P1015T/Y1019L, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0027 | - |
phenanthridine | mutant F1014I, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0031 | - |
N1-methyl-nicotineamide | wild-type, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0037 | - |
phenanthridine | mutant F1014V, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0119 | - |
allopurinol | mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0119 | - |
Phthalazine | mutant F776K/A807E/D878L/L881S/Y885R/K889H/P1015T/Y1019L, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0124 | - |
N1-methyl-nicotineamide | mutant P1015A, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.013 | - |
benzaldehyde | mutant F1014I, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.0216 | - |
N1-methyl-nicotineamide | mutant P1015G, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.044 | - |
phenanthridine | wild-type, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.098 | - |
benzaldehyde | mutant F1014V, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.098 | - |
phenanthridine | mutant P1015A, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.11 | - |
Phthalazine | mutant F1014I, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.189 | - |
phenanthridine | mutant P1015G, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.37 | - |
Phthalazine | mutant F1014V, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.45 | - |
benzaldehyde | wild-type, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.47 | - |
benzaldehyde | mutant P1015G, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.56 | - |
Phthalazine | mutant P1015G, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.58 | - |
benzaldehyde | mutant P1015A, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 0.79 | - |
Phthalazine | wild-type, pH 8.0,37°C | Mus musculus | |
1.2.3.1 | 1.148 | - |
Phthalazine | mutant P1015A, pH 8.0,37°C | Mus musculus |