EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.195 | gene ctcad1, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative realtime PCR enzyme expression analysis | Carthamus tinctorius |
1.1.1.195 | gene ctcad2, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative realtime PCR enzyme expression analysis | Carthamus tinctorius |
1.1.1.195 | quantitative realtime PCR enzyme expression analysis during seed development, overview. CAD2 shows the highest expression level of all three CAD isozymes in Carthamus tinctorius | Carthamus tinctorius |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.195 | coniferyl aldehyde | competitve, a stronger inhibitor than sinapyl aldehyde; competitve, a stronger inhibitor than sinapyl aldehyde; competitve, a stronger inhibitor than sinapyl aldehyde | Carthamus tinctorius | |
1.1.1.195 | sinapyl aldehyde | competitve, a weaker inhibitor than coniferyl aldehyde; competitve, a weaker inhibitor than coniferyl aldehyde; competitve, a weaker inhibitor than coniferyl aldehyde | Carthamus tinctorius |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.195 | 0.0058 | - |
sinapyl aldehyde | pH 6.5-7.5, 26°C, recombinant enzyme | Carthamus tinctorius | |
1.1.1.195 | 0.0068 | - |
coniferyl aldehyde | pH 6.5-7.5, 26°C, recombinant enzyme | Carthamus tinctorius | |
1.1.1.195 | 0.024 | - |
coniferyl aldehyde | pH 6.5-7.5, 30°C, recombinant enzyme | Carthamus tinctorius | |
1.1.1.195 | 0.026 | - |
sinapyl aldehyde | pH 6.5-7.5, 30°C, recombinant enzyme | Carthamus tinctorius | |
1.1.1.195 | 0.032 | - |
coniferyl aldehyde | pH 6.5-7.5, 30°C, recombinant enzyme | Carthamus tinctorius | |
1.1.1.195 | 0.055 | - |
sinapyl aldehyde | pH 6.5-7.5, 30°C, recombinant enzyme | Carthamus tinctorius |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.195 | Zn2+ | required, Zn1 binding sequence is located at amino acid residues 68-82, and Zn2 binding sequence is located at amino acid residues 88-113 | Carthamus tinctorius | |
1.1.1.195 | Zn2+ | required, Zn1 binding sequence is located at amino acid residues 69-83, and Zn2 binding sequence is located at amino acid residues 89-114 | Carthamus tinctorius | |
1.1.1.195 | Zn2+ | required, Zn1 binding sequence is located at amino acid residues 71-85, and Zn2 binding sequence is located at amino acid residues 91-116 | Carthamus tinctorius |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.195 | cinnamaldehyde + NADPH + H+ | Carthamus tinctorius | - |
cinnamyl alcohol + NADP+ | - |
r | |
1.1.1.195 | coniferyl aldehyde + NADPH + H+ | Carthamus tinctorius | - |
coniferyl alcohol + NADP+ | - |
r | |
1.1.1.195 | sinapyl aldehyde + NADPH + H+ | Carthamus tinctorius | - |
sinapyl alcohol + NADP+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.195 | Carthamus tinctorius | U3TDQ6 | CAD3 | - |
1.1.1.195 | Carthamus tinctorius | U3TH11 | CAD2 | - |
1.1.1.195 | Carthamus tinctorius | U3TI46 | CAD1 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.195 | seed | quantitative realtime PCR enzyme expression analysis during seed development, overview. CAD2 shows the highest expression level of all three CAD isozymes in Carthamus tinctorius | Carthamus tinctorius | - |
1.1.1.195 | stem | - |
Carthamus tinctorius | - |
1.1.1.195 | stem | low expression of CAD3 in stem | Carthamus tinctorius | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.195 | cinnamaldehyde + NADPH + H+ | - |
Carthamus tinctorius | cinnamyl alcohol + NADP+ | - |
r | |
1.1.1.195 | coniferyl aldehyde + NADPH + H+ | - |
Carthamus tinctorius | coniferyl alcohol + NADP+ | - |
r | |
1.1.1.195 | sinapyl aldehyde + NADPH + H+ | - |
Carthamus tinctorius | sinapyl alcohol + NADP+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.195 | CAD | - |
Carthamus tinctorius |
1.1.1.195 | cinnamyl alcohol dehydrogenase | - |
Carthamus tinctorius |
1.1.1.195 | CtCAD1 | - |
Carthamus tinctorius |
1.1.1.195 | CtCAD2 | - |
Carthamus tinctorius |
1.1.1.195 | CtCAD3 | - |
Carthamus tinctorius |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.195 | 26 | - |
assay at | Carthamus tinctorius |
1.1.1.195 | 30 | - |
assay at | Carthamus tinctorius |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.195 | 0.00098 | - |
coniferyl aldehyde | pH 6.5-7.5, 30°C, recombinant enzyme | Carthamus tinctorius | |
1.1.1.195 | 0.00135 | - |
sinapyl aldehyde | pH 6.5-7.5, 30°C, recombinant enzyme | Carthamus tinctorius | |
1.1.1.195 | 0.0053 | - |
sinapyl aldehyde | pH 6.5-7.5, 26°C, recombinant enzyme | Carthamus tinctorius | |
1.1.1.195 | 0.0121 | - |
coniferyl aldehyde | pH 6.5-7.5, 26°C, recombinant enzyme | Carthamus tinctorius | |
1.1.1.195 | 0.032 | - |
coniferyl aldehyde | pH 6.5-7.5, 30°C, recombinant enzyme | Carthamus tinctorius | |
1.1.1.195 | 0.041 | - |
sinapyl aldehyde | pH 6.5-7.5, 30°C, recombinant enzyme | Carthamus tinctorius |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.195 | 6.5 | 7.5 | assay at | Carthamus tinctorius |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.195 | NADP+ | - |
Carthamus tinctorius | |
1.1.1.195 | NADPH | the NADPH binding sequence is located at amino acid residues 188-193 | Carthamus tinctorius | |
1.1.1.195 | NADPH | the NADPH binding sequence is located at amino acid residues 189-194 | Carthamus tinctorius | |
1.1.1.195 | NADPH | the NADPH binding sequence is located at amino acid residues 191-196 | Carthamus tinctorius |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.195 | additional information | enzyme structure modelling and molecular docking, substrate binding pocket structure, overview | Carthamus tinctorius |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.195 | 0.0245 | - |
sinapyl aldehyde | pH 6.5-7.5, 30°C, recombinant enzyme | Carthamus tinctorius | |
1.1.1.195 | 0.0306 | - |
coniferyl aldehyde | pH 6.5-7.5, 30°C, recombinant enzyme | Carthamus tinctorius | |
1.1.1.195 | 1.32 | - |
coniferyl aldehyde | pH 6.5-7.5, 30°C, recombinant enzyme | Carthamus tinctorius | |
1.1.1.195 | 1.58 | - |
sinapyl aldehyde | pH 6.5-7.5, 30°C, recombinant enzyme | Carthamus tinctorius | |
1.1.1.195 | 5.39 | - |
sinapyl aldehyde | pH 6.5-7.5, 26°C, recombinant enzyme | Carthamus tinctorius | |
1.1.1.195 | 10.62 | - |
coniferyl aldehyde | pH 6.5-7.5, 26°C, recombinant enzyme | Carthamus tinctorius |