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Literature summary extracted from

  • Yamada, M.; Adachi, K.; Ogawa, N.; Kishino, S.; Ogawa, J.; Kataoka, M.; Shimizu, S.; Isobe, K.
    A new aldehyde oxidase catalyzing the conversion of glycolaldehyde to glycolate from Burkholderia sp. AIU 129 (2015), J. Biosci. Bioeng., 119, 410-415.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.3.1 8-hydroxyquinoline 1 mM, 20% residual activity Burkholderia sp. AIU 129
1.2.3.1 alpha,alpha'-dipyridyl 1 mM, 49% residual activity Burkholderia sp. AIU 129
1.2.3.1 Cu2+ 1 mM, 4% residual activity Burkholderia sp. AIU 129
1.2.3.1 Fe2+ 1 mM, no residual activity Burkholderia sp. AIU 129
1.2.3.1 Mo5+ 1 mM, 6% residual activity Burkholderia sp. AIU 129
1.2.3.1 phenylhydrazine 1 mM, 6% residual activity Burkholderia sp. AIU 129
1.2.3.1 Zn2+ 1 mM, 67% residual activity Burkholderia sp. AIU 129

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.3.1 0.06
-
benzaldehyde pH 7.0, 30°C Burkholderia sp. AIU 129
1.2.3.1 0.1
-
butylaldehyde pH 7.0, 30°C Burkholderia sp. AIU 129
1.2.3.1 0.34
-
acetaldehyde pH 7.0, 30°C Burkholderia sp. AIU 129
1.2.3.1 0.74
-
propionaldehyde pH 7.0, 30°C Burkholderia sp. AIU 129
1.2.3.1 1
-
glycolaldehyde pH 7.0, 30°C Burkholderia sp. AIU 129
1.2.3.1 12.3
-
Glyoxal pH 7.0, 30°C Burkholderia sp. AIU 129
1.2.3.1 35.9
-
formaldehyde pH 7.0, 30°C Burkholderia sp. AIU 129

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.3.1 14000
-
-
Burkholderia sp. AIU 129
1.2.3.1 36000
-
-
Burkholderia sp. AIU 129
1.2.3.1 76000
-
-
Burkholderia sp. AIU 129
1.2.3.1 130000
-
gel filtration Burkholderia sp. AIU 129

Organism

EC Number Organism UniProt Comment Textmining
1.2.3.1 Burkholderia sp. AIU 129
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.3.1 acetaldehyde + O2 + H2O
-
Burkholderia sp. AIU 129 acetate + H2O2
-
?
1.2.3.1 benzaldehyde + H2O + O2
-
Burkholderia sp. AIU 129 benzoate + H2O2
-
?
1.2.3.1 butylaldehyde + H2O + O2
-
Burkholderia sp. AIU 129 butanoate + H2O2
-
?
1.2.3.1 formaldehyde + H2O + O2
-
Burkholderia sp. AIU 129 formate + H2O2
-
?
1.2.3.1 glycolaldehyde + H2O + O2
-
Burkholderia sp. AIU 129 hydroxyacetic acid + H2O2
-
?
1.2.3.1 glyoxal + H2O + O2
-
Burkholderia sp. AIU 129 glyoxylate + H2O2
-
?
1.2.3.1 additional information no substrates: glycolate and alcohols such as ethanol, 1-propanol, 2-methoxyethanol, ethylene glycol and glycerol Burkholderia sp. AIU 129 ?
-
?
1.2.3.1 propionaldehyde + H2O + O2
-
Burkholderia sp. AIU 129 propionate + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.2.3.1 heterotrimer 1 * 76000, alpha-subunit, plus 1 * 36000, beta-subunit, plus 1 * 14000, gamma-subunit Burkholderia sp. AIU 129

Synonyms

EC Number Synonyms Comment Organism
1.2.3.1 ALOD
-
Burkholderia sp. AIU 129

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.3.1 50
-
-
Burkholderia sp. AIU 129

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.3.1 70
-
30 min, morethan 50% residual activity Burkholderia sp. AIU 129

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.3.1 6
-
-
Burkholderia sp. AIU 129

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.3.1 7 8 more than 70% of maximum activity Burkholderia sp. AIU 129