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Literature summary extracted from
- Molecular cloning and biochemical characterization of two cinnamyl alcohol dehydrogenases from a liverwort Plagiochasma appendiculatum (2013), Plant Physiol. Biochem., 70, 133-141.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.195 | gene PaCAD1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Plagiochasma appendiculatum |
| 1.1.1.195 | gene PaCAD2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Plagiochasma appendiculatum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.195 | 0.03115 | - |
coniferaldehyde | isoform CAD2, at pH 6.0 and 25°C | Plagiochasma appendiculatum |  |
| 1.1.1.195 | 0.0382 | - |
4-coumarylaldehyde | isoform CAD2, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.06095 | - |
sinapaldehyde | isoform CAD2, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.06592 | - |
caffeylaldehyde | isoform CAD2, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.08554 | - |
coniferaldehyde | isoform CAD1, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.09157 | - |
sinapaldehyde | isoform CAD1, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.09318 | - |
5-hydroxyconiferylaldehyde | isoform CAD1, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.09463 | - |
caffeylaldehyde | isoform CAD1, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.1122 | - |
4-coumarylaldehyde | isoform CAD1, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.152 | - |
5-hydroxyconiferylaldehyde | isoform CAD2, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.195 | 35700 | - |
- |
Plagiochasma appendiculatum |
| 1.1.1.195 | 36900 | - |
- |
Plagiochasma appendiculatum |
| 1.1.1.195 | 55000 | 60000 | gel filtration | Plagiochasma appendiculatum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.195 | Plagiochasma appendiculatum | - |
- |
- |
| 1.1.1.195 | Plagiochasma appendiculatum | T1SEU9 | - |
- |
| 1.1.1.195 | Plagiochasma appendiculatum | T1SEV0 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.195 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chrmatography | Plagiochasma appendiculatum |
| 1.1.1.195 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Plagiochasma appendiculatum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.195 | 4-coumarylaldehyde + NADPH + H+ | high catalytic efficiency | Plagiochasma appendiculatum | 4-coumaryl alcohol + NADP+ | - |
? | |
| 1.1.1.195 | 5-hydroxyconiferyl aldehyde + NADP+ | very low activity | Plagiochasma appendiculatum | 5-hydroxyconiferyl alcohol + NADPH + H+ | - |
? | |
| 1.1.1.195 | 5-hydroxyconiferylaldehyde + NADP+ | very low activity | Plagiochasma appendiculatum | 5-hydroxyconiferyl alcohol + NADPH + H+ | - |
? | |
| 1.1.1.195 | 5-hydroxyconiferylaldehyde + NADPH + H+ | lowest catalytic efficiency | Plagiochasma appendiculatum | 5-hydroxyconiferyl alcohol + NADP+ | - |
? | |
| 1.1.1.195 | caffeylaldehyde + NADP+ | low activity | Plagiochasma appendiculatum | caffeyl alcohol + NADPH + H+ | - |
? | |
| 1.1.1.195 | caffeylaldehyde + NADPH + H+ | - |
Plagiochasma appendiculatum | caffeyl alcohol + NADP+ | - |
? | |
| 1.1.1.195 | coniferaldehyde + NADPH + H+ | favourite substrate | Plagiochasma appendiculatum | coniferyl alcohol + NADP+ | - |
? | |
| 1.1.1.195 | coniferyl aldehyde + NADPH + H+ | best substrate | Plagiochasma appendiculatum | coniferyl alcohol + NADP+ | - |
? | |
| 1.1.1.195 | p-coumaryl aldehyde + NADPH + H+ | - |
Plagiochasma appendiculatum | p-coumaryl alcohol + NADP+ | - |
? | |
| 1.1.1.195 | p-coumarylaldehyde + NADPH + H+ | - |
Plagiochasma appendiculatum | p-coumaryl alcohol + NADP+ | - |
? | |
| 1.1.1.195 | sinapaldehyde + NADPH + H+ | - |
Plagiochasma appendiculatum | sinapyl alcohol + NADP+ | - |
? | |
| 1.1.1.195 | sinapyl aldehyde + NADPH + H+ | low activity | Plagiochasma appendiculatum | sinapyl alcohol + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.195 | ? | x * 35700, about, CAD1, sequence calculation | Plagiochasma appendiculatum |
| 1.1.1.195 | ? | x * 36900, about, CAD2, sequence calculation | Plagiochasma appendiculatum |
| 1.1.1.195 | homodimer | 2 * 35700, isoform CAD1, calculated from amino acid sequence | Plagiochasma appendiculatum |
| 1.1.1.195 | homodimer | 2 * 36900, isoform CAD2, calculated from amino acid sequence | Plagiochasma appendiculatum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.195 | CAD1 | - |
Plagiochasma appendiculatum |
| 1.1.1.195 | CAD1 | isoform | Plagiochasma appendiculatum |
| 1.1.1.195 | CAD2 | - |
Plagiochasma appendiculatum |
| 1.1.1.195 | CAD2 | isoform | Plagiochasma appendiculatum |
| 1.1.1.195 | cinnamyl alcohol dehydrogenase | - |
Plagiochasma appendiculatum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.195 | 25 | - |
- |
Plagiochasma appendiculatum |
| 1.1.1.195 | 25 | - |
isoform CAD1 | Plagiochasma appendiculatum |
| 1.1.1.195 | 30 | - |
- |
Plagiochasma appendiculatum |
| 1.1.1.195 | 30 | - |
isoform CAD2 | Plagiochasma appendiculatum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.195 | 0.005 | - |
5-hydroxyconiferylaldehyde | isoform CAD1, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.005 | - |
sinapaldehyde | isoform CAD2, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.006 | - |
sinapaldehyde | isoform CAD1, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.007 | - |
5-hydroxyconiferylaldehyde | isoform CAD2, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.062 | - |
caffeylaldehyde | isoform CAD2, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.072 | - |
caffeylaldehyde | isoform CAD1, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.072 | - |
coniferaldehyde | isoform CAD2, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.085 | - |
coniferaldehyde | isoform CAD1, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.273 | - |
4-coumarylaldehyde | isoform CAD2, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.367 | - |
4-coumarylaldehyde | isoform CAD1, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.195 | 5.5 | - |
aldehyde reduction | Plagiochasma appendiculatum |
| 1.1.1.195 | 5.5 | - |
isoform CAD2 | Plagiochasma appendiculatum |
| 1.1.1.195 | 6 | - |
aldehyde reduction | Plagiochasma appendiculatum |
| 1.1.1.195 | 6 | - |
isoform CAD1 | Plagiochasma appendiculatum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.195 | NADP+ | - |
Plagiochasma appendiculatum | |
| 1.1.1.195 | NADPH | - |
Plagiochasma appendiculatum | |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.1.1.195 | Plagiochasma appendiculatum | the expression of isoforms CAD1 and CAD2 is induced by methyl jasmonic acid treatment | up |
| 1.1.1.195 | Plagiochasma appendiculatum | the expression of PaCAD1 is induced by methyl jasmonic acid (MeJA) treatment by about 2fold | up |
| 1.1.1.195 | Plagiochasma appendiculatum | the expression of PaCAD2 is induced by methyl jasmonic acid (MeJA) treatment by up to 7fold | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.1.1.195 | physiological function | the enzyme plays a role in lignin biosynthesis and the defencedefence of abiotic stresses | Plagiochasma appendiculatum |
| 1.1.1.195 | physiological function | the two PaCADs, PaCAD1 and PaCAD2, play twin roles in lignin biosynthesis and the defence of abiotic stress in Plagiochasma appendiculatum | Plagiochasma appendiculatum |
| 1.1.1.195 | physiological function | the two PaCADs, PaCAD1 and PaCAD2, play twin roles in lignin biosynthesis and the defencedefence of abiotic stress in Plagiochasma appendiculatum | Plagiochasma appendiculatum |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.195 | 0.0448 | - |
5-hydroxyconiferylaldehyde | isoform CAD2, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.054 | - |
5-hydroxyconiferylaldehyde | isoform CAD1, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.063 | - |
sinapaldehyde | isoform CAD1, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.075 | - |
sinapaldehyde | isoform CAD2, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.766 | - |
caffeylaldehyde | isoform CAD1, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.935 | - |
caffeylaldehyde | isoform CAD2, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 0.993 | - |
coniferaldehyde | isoform CAD1, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 2.322 | - |
coniferaldehyde | isoform CAD2, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 3.273 | - |
4-coumarylaldehyde | isoform CAD1, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
| 1.1.1.195 | 7.136 | - |
4-coumarylaldehyde | isoform CAD2, at pH 6.0 and 25°C | Plagiochasma appendiculatum | |
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