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Literature summary extracted from

  • Tomoike, F.; Kuramitsu, S.; Masui, R.
    Unique substrate specificity of purine nucleoside phosphorylases from Thermus thermophilus (2013), Extremophiles, 17, 505-514.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.1 expressed in Escherichia coli Rosetta(DE3) cells Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.1 A196E/D238N the mutations in the trimeric enzyme form change substrate specificity from guanosine to adenosine Thermus thermophilus
2.4.2.1 N204D the mutation in the hexameric enzyme form changes substrate specificity from adenosine to guanosine Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.0071
-
 guanosine wild type hexameric enzyme, at pH 8.0 and 70°C Thermus thermophilus
2.4.2.1 0.083
-
 adenosine wild type trimeric enzyme, at pH 8.0 and 70°C Thermus thermophilus
2.4.2.1 0.097
-
 adenosine mutant hexameric enzyme N204D, at pH 8.0 and 70°C Thermus thermophilus
2.4.2.1 0.407
-
 guanosine mutant trimeric enzyme A196E/D238N, at pH 8.0 and 70°C Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Thermus thermophilus
-
-
-
2.4.2.1 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1 Toyopearl Phenyl column chromatography, Toyopearl SuperQ column chromatography, and Superdex 200 gel filtration Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 adenosine + phosphate exclusive substrate for the hexameric enzyme Thermus thermophilus adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 adenosine + phosphate exclusive substrate for the hexameric enzyme Thermus thermophilus HB8 / ATCC 27634 / DSM 579 adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate exclusive substrate for the trimeric enzyme Thermus thermophilus guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate exclusive substrate for the trimeric enzyme Thermus thermophilus HB8 / ATCC 27634 / DSM 579 guanine + alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.1 trimer or hexamer
-
 Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
2.4.2.1 PNP
-
 Thermus thermophilus
2.4.2.1 purine nucleoside phosphorylase
-
 Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.1 0.22
-
 adenosine mutant hexameric enzyme N204D, at pH 8.0 and 70°C Thermus thermophilus
2.4.2.1 0.25
-
 guanosine mutant trimeric enzyme A196E/D238N, at pH 8.0 and 70°C Thermus thermophilus
2.4.2.1 0.26
-
 guanosine wild type hexameric enzyme, at pH 8.0 and 70°C Thermus thermophilus
2.4.2.1 0.55
-
 adenosine wild type trimeric enzyme, at pH 8.0 and 70°C Thermus thermophilus